No abstract available.

No abstract available.

The ovarian specimens obtained from the patients with 27 malignant germ cell tumors were analyzed in order to study DNA and form factor using flow cytometry and image analyzer. The malignant germ cell tumors consisted of six dysgerminomas, six endodermal sinus tumors, seven immature teratomas, three teratomas associated with squamous cell carcinoma, two embryonal carcinomas one mixed germ cell tumor and one malignant struma ovarii. Five normal ovaries used as control group. Various prognostic factors such as DNA ploidy, S-Phase and measurements of from were evaluated by flow cytometry and image analyzer. Mitotic index, histological grade, nuclear grade and tumor necrosis were assessed with microscope. These prognostic factors of DNA ploidy, S-phase, form factor histological were compared with tumor recurrence and clinical stage in this study. The results were as follows: 1. Sixteen(59%) out of 27 were aneuploidy and 11(41%) diploidy. All six dysgerminomas and six endodermal sinus tumors were aneuploidy, while six immature teratomas were diploidy. 2. S-phase fractions of malignant germ cell related significantly with those mitotic index(P=0.0201). S-phase fractions significantly increased in mitotic index gradeIII compared with grade I(P<0.01). There was no significant different between grade Iand II, and between gradeII and III. There was no difference between S-phase fractions and the remaining histological variables. 3. The incidence of aneuploidy was higher in the high s_ phase fractions(P=0.0041). However, there was no difference between S-phase fractions and tumor recurrence. 4. The incidence of aneuploidy significantly increased in clinical stage III and IV compared with stage I and II(P=0.0368). However, the difference between clinical stage histological variables was not significant. 5. The difference between form factor and histological variables, between from and tumor recurrence(P=0.3698), and between from factor and S-phase fractions(r=0.76) could not reach statistical significance. These results suggest that ploidy can give significant value for routine clinical prognostic prediction, whereas hitologic variables and form factor are poorly suitable for the prognostic evaluation.
Aneuploidy ; Carcinoma, Embryonal ; Carcinoma, Squamous Cell ; Diploidy ; DNA* ; Dysgerminoma ; Endodermal Sinus Tumor ; Female ; Flow Cytometry ; Germ Cells* ; Humans ; Incidence ; Mitotic Index ; Necrosis ; Neoplasms, Germ Cell and Embryonal* ; Ovary* ; Ploidies* ; Recurrence ; Struma Ovarii ; Teratoma

The ovarian specimens obtained from the patients with 27 malignant germ cell tumors were analyzed in order to study DNA and form factor using flow cytometry and image analyzer. The malignant germ cell tumors consisted of six dysgerminomas, six endodermal sinus tumors, seven immature teratomas, three teratomas associated with squamous cell carcinoma, two embryonal carcinomas one mixed germ cell tumor and one malignant struma ovarii. Five normal ovaries used as control group. Various prognostic factors such as DNA ploidy, S-Phase and measurements of from were evaluated by flow cytometry and image analyzer. Mitotic index, histological grade, nuclear grade and tumor necrosis were assessed with microscope. These prognostic factors of DNA ploidy, S-phase, form factor histological were compared with tumor recurrence and clinical stage in this study. The results were as follows: 1. Sixteen(59%) out of 27 were aneuploidy and 11(41%) diploidy. All six dysgerminomas and six endodermal sinus tumors were aneuploidy, while six immature teratomas were diploidy. 2. S-phase fractions of malignant germ cell related significantly with those mitotic index(P=0.0201). S-phase fractions significantly increased in mitotic index gradeIII compared with grade I(P<0.01). There was no significant different between grade Iand II, and between gradeII and III. There was no difference between S-phase fractions and the remaining histological variables. 3. The incidence of aneuploidy was higher in the high s_ phase fractions(P=0.0041). However, there was no difference between S-phase fractions and tumor recurrence. 4. The incidence of aneuploidy significantly increased in clinical stage III and IV compared with stage I and II(P=0.0368). However, the difference between clinical stage histological variables was not significant. 5. The difference between form factor and histological variables, between from and tumor recurrence(P=0.3698), and between from factor and S-phase fractions(r=0.76) could not reach statistical significance. These results suggest that ploidy can give significant value for routine clinical prognostic prediction, whereas hitologic variables and form factor are poorly suitable for the prognostic evaluation.
Aneuploidy ; Carcinoma, Embryonal ; Carcinoma, Squamous Cell ; Diploidy ; DNA* ; Dysgerminoma ; Endodermal Sinus Tumor ; Female ; Flow Cytometry ; Germ Cells* ; Humans ; Incidence ; Mitotic Index ; Necrosis ; Neoplasms, Germ Cell and Embryonal* ; Ovary* ; Ploidies* ; Recurrence ; Struma Ovarii ; Teratoma

No abstract available.

No abstract available.

No abstract available.

No abstract available.

No abstract available.

STUDY DESIGN: Thirty-four cases of surgically treated spinal stenosis which were followed up for more than 5 yeras were analysed. OBJECTIVES: To know the relationship between long-term clinical results and various clinical factors. SUMMARY OF LITERATURE REVIEW: Pedicle screw fixation is a common procedure after wide decompression for spinal stenosis. But, there is few report showing its long-term result. MATERIALS AND METHODS: We evaluated the long term results of surgically treated spinal stenosis patients according to the sex, method of fusion, level of fusion, radiologic change of adjacent level, hardware problem and complication. There were 11 males and 23 females. Average age was 53.2 years(range 34-68 years). Radiographically follow up was averaged 72 months(range 60-104 months). Clinically follow up period was averaged 76 months(range 60-113 months). Eight patients were operated with PLIF and 26 patients were with posterolateral fusion. One level surgery was done in 18 patient and more than two level in 16 patients. RESULTS: Twenty-seven patients(79%) showed satisfactory result at 2year and 5 year follow-up. Two patients showed change of clinical result during follow-up. Clinical result was much better in monosegmental surgery and in male sex. Six patients showed narrowing of disc space at non-surgery level and 2 patients had compression fracture. Complications were 1 deep infection, 2 dural tears, 9 donor site pain, 2 metal failures and 1 metal pull-out. CONCLUSIONS: We concluded that there is no significant change of clinical results between two year follow-up and five year follow-up. Clinical complications and metal problems are not related with the unsatisfactory results. Multiple level surgery and female sex are two variables related with the unsatisfactory clinical outcome.
Decompression* ; Female ; Follow-Up Studies* ; Fractures, Compression ; Humans ; Male ; Spinal Stenosis* ; Tissue Donors