No abstract available.
Korea*

We examined the fibronectin (FN) secretion of cultured trabecular meshwork (TM) cells in a normal human eye by indirect immunofluorescent technique using mouse anti-human FN monoclonal antibody and FITC-conjugated goat anti-mouse IgG. To localize FN on frozen sections of normal TM, which were obtained from 7 enucleated eyes owing to traumatic eyeball rupture, the same indirect immunofluorescent method was used. Immunoelectron microscopy was applied to demonstrate the distribution pattern of FN in the normal TM of 2 human eyes using an avidin-biotin-peroxidase complex method. In the tissue culture of TM, the TM cell walls and extracellular matrices showed an intense staining with antibody to FN. Indirect immunofluorescent staining of FN on frozen sections of TM showed strong positive reactions in the subendothelial region. There was no reaction in the central core of the trabecular beam. Immunoelectron microscopy revealed the reaction products to FN in the areas lining the trabecular endothelial cells.
Adolescent ; Adult ; Antibodies, Monoclonal ; Cells, Cultured ; Fibronectins/biosynthesis/*metabolism ; Fluorescent Antibody Technique ; Humans ; Male ; Microscopy, Immunoelectron ; Trabecular Meshwork/*metabolism/ultrastructure

We examined the fibronectin(FN) secretion by cultured trabecular meshwork(TM) cells from a normal human eye by indirect immunofluorescent technique using mouse anti-human FN monoclonal antibody and FITC-conjugated goat anti-mouse IgG. To localize FN on frozen sections of normal TM which were obtained from 7 enucleated eyes owing to traumatic eyeball rupture, the same indirect immunofluorescent method was used. Immunoelectron microscopy was applied to demonstrate the distribution pattern of FN in the normal TM of 2 human eyes using an avidin-biotin-peroxidase complex method. In tissue culture of TM, TM cell walls and extracellular matrices showed an intense staining with antibody to FN. Indirect immunofluorescent staining of FN on frozen sections of TM showed strong positive reactions in the subendothelial region. There was no reaction in the central core of the trabecular beam. Immunoelectron microscopy revealed the reaction products to FN in the areas lining the trabecular endothelial cells.
Animals ; Cell Wall ; Endothelial Cells ; Extracellular Matrix ; Fibronectins* ; Fluorescent Antibody Technique, Indirect ; Frozen Sections ; Goats ; Humans ; Immunoglobulin G ; Mice ; Microscopy, Immunoelectron ; Rupture ; Trabecular Meshwork*

A study of histopathologic changes, ultrastructure, and expression of the HLA-Dr antigen within the giant papillae of patients with giant papillary conjunctivitis was performed to determine whether cell-mediated immune response is related to this condition. Conjunctival giant papillae from ten patients with giant papillary conjunctivitis were examined by light and electron microscopy and by the indirect immunofluorescent staining method with HLA-Dr antibody. The infiltration of eosinophilic neutrophils and granules was most prominent, with the occasional infiltration of mast cells, as shown by light microscopy. The infiltration of activated fibroblasts and Langerhans cells was also observed. Cells expressing HLA-Dr antigen were also markedly increased, as shown by the immunofluorescent method. These findings suggest that delayed hypersensitivity may, along with the processes of antigen presentation by HLA-Dr-expressing (including Langerhans) cells, contribute to the pathogenesis of giant papillary conjunctivitis.
Adolescent ; Adult ; Child ; Conjunctiva/metabolism/*ultrastructure ; Conjunctivitis, Allergic/*metabolism/*pathology ; Fluorescent Antibody Technique, Indirect ; HLA-DR Antigens/*biosynthesis ; Humans ; Immunity, Cellular ; Langerhans Cells/*ultrastructure ; Microscopy, Electron ; Middle Aged

A twenty year old male patient was exposed on his right temporal portion of head to 22,000 volt alternating electric current on December 12, 1978. He lost consciousness for about three days. He sustained second and third degree burn on exposed site and right lower extremity. Right lower leg was amputated months later at orthopedic deparment. And diminution of vision began to be felt on April 1979. So he visited to our ophthalmic O.P.D. on June 11, 1979. On the first ocular examination, the visual acuity of both eyes was similar -Hand Movement- and the intraocular pressure of right was 14.6 mmHg and that of the left was 17.3 mmHg. And light projection and color perception of both eyes were good. There were multiple round punctate opacities in anterior subcapsular region and total opacification of lens cortex were also noted in both eyes by slit lamp examination. On June 12, 1979, intracapsular lens cryoextraction of right eye was done with Zonolysin(R) application and five days later left eye was operated by the same procedure. Corrected visual acuity of both eyes was 20/20 without significant complication.
Burns ; Cataract* ; Color Perception ; Consciousness ; Head ; Humans ; Intraocular Pressure ; Leg ; Lower Extremity ; Male ; Orthopedics ; Visual Acuity

The authors recovered a white thread-like living nematode in left conjunctival sac of a 33 years old male on May 29, 1981. The chief complaints were foreign body sensation and itching sensation of eye. The worm was a female measuring 12.1 mm in length and 0.171 mm in maximum width. It was identified as Thelazia callipaeda Railliet et Henry, 1910. And we recorded the patient as the 10th reported thelaziasis case in Korea.
parasitology-helminth-nematoda ; Thelazia callipaeda ; thelaziasis ; eye ; conjunctival sac ; case report

PURPOSE: Developments in micro/macrostructures of implants and surgical techniques brought out stable outcomes of implant dentistry. The aim of this study was to evaluate the distributions of implant patients, the types of implanted sites, and the success or survival rates of various implant systems and to analyze the implant placement done at each specificintraoral site and situation. MATERIALS AND METHODS: The data of dental implantations collected between 1992 and 2006 at the Department of Periodontology in 00000 University Hospital were analyzed. RESULTS: 1. Largest part of the patients were at the age of 40s and 50s in bothgender who lost their teeth mostly by periodontaldiseases and caries at the posterior intraoral sites as major ones. Bone densities of type II(mandible) and III(maxilla) were likely to be seen with quantity of type B. Lengths of the implants between 10 and 15 mm and wide platform took the largest part. 2. Survival rates of Implantium(R)(98.8%), Xive(R)(100%) and ITI TE(R)(100%) were high when Frialit-2(R) showed 82%(poor bone density area) or 87.2%(combined with additional therapy). IMZ(R) had lowest cumulative survival(67.5%) and success rate(49.4%) amongst all. 3. Replacement with 2 wide or 3 regular platforms showed no significant differences in survival rate and marginal bone loss atmandibular posterior area. In single restoration of mandibular second molar, 5-year success rate of machined surface Branemark(R)(70.37%) was lower than that of rough surface ITI(R) SLA(100%). 4. Replacement of single tooth in anterior area showed high survival rate of 94.5%. 5. The success rates of Branemark Ti-Unite and ITI SLA at posterior maxilla with poor bone density both showed stable outcomes. 6. 10-year cumulative survival rate of implants with maxillary sinus augmentation by lateral window approach appeared to be 96.60%. Low survival rate(75%) was shown when there were more than two complications combined. Height of grafted bone remained stable above the implant apex. CONCLUSIONS : Rough surfaced implants showed stable outcomes in most of the situation including poor bone density and additional therapy combined.
Bone Density ; Dental Implants* ; Dentistry ; Humans ; Maxilla ; Maxillary Sinus ; Molar ; Retrospective Studies* ; Survival Rate ; Tooth ; Transplants

Bone morphogenetic proteins have been shown to possess significant osteoinSductive potential, but in order to take advantage of this effect for tissue engineering, carrier systems are essential. Successful carrier systems must enable vascular and cellular invasion, allowing BMP to act as a differentiation factor. The carrier should be reproducible, non-immunogenic, moldable, and space-providing, to define the contours of the resulting bone. The purpose of this study was to review available literature, in comparing various carriers of BMP on rat calvarial defect model. The following conclusions were deduced. 1. Bone regeneration of ACS/BMP, beta-TCP/BMP, FFSS/BMP, FFSS/beta-TCP/BMP, MBCP/BMP group were significantly greater than the control groups. 2. Bone density in the ACS/BMP group was greater than that in beta-TCP, FFSS, FFSS/beta-TCP carrier group. 3. Bone regeneration in FFSS/BMP group was less than in ACS/BMP, beta-TCP/BMP, MBCP/BMP group. However, New bone area of FFSS/beta-TCP/BMP carrier group were more greater than that of FFSS/BMP group. ACS, beta-TCP, FFSS, FFSS/beta-TCP, MBCP were used for carrier of BMP. However, an ideal carrier which was reproducible, non-immunogenic, moldable, and space-providing did not exist. Therefore, further investigation are required in developing a new carrier system.
Animals ; Bone Density ; Bone Morphogenetic Proteins* ; Bone Regeneration ; Rats* ; Tissue Engineering

PURPOSE: Various bone graft materials are being used for periodontal tissue regeneration.Th materials are being developed continuously for ideal clinical effects. Therefore, it is necessary to identify the clinical characteristics of each bone graft material through comparing the various bone graft materials statistically and in doing so, proposing a more efficient bone graft material. In this study, the following results were attained through comparing the clinical effects among the bone graft materials, using the statistical method based on the clinical studies published at the department of periodontology of Yonsei hospital. MATERIALS AND METHOD: 6 selected studies of department of Periodontology at Yonsei University Hospital were based on clinical study of bone grafting in intrabony defects. It was compared the clinical parameters among the 6 clinical studies, using the statistical META analysis. RESULT: When comparing the probing depth reduction, there was a relatively great amount of decrease when using the xenograft, Anorganic Bovine Derived Hydroxapatite Bone Matrix/Cell Binding Peptide(ABM/P-15: PepGen P-15(R)) and the autogenous bone and absorbable membrane, d,l-alctide/glycolide copolymer(GC: Biomesh(R)). The allogfrafts showed a relatively low decrease in the probing depth and clinical attachment change. It also showed a slight decrease in the bone probing depth. The allografts showed various results according to different bone graft materials. When comparing the ABM/P-15 and bovine bone powder(BBP(R)), ABM/P-15 showed a relatively high clinical attachment level and the bovine bone powder showed a relatively high clinical attachment level. The probing depth change and gingival recession change showed a lower value than the mean value between the two bone graft materials. The synthetic bone showed a relatively high decrease in clinical attachment level and periodontal probing depth change. There was a relatively larger amount of gingival recession when using Bioactive Glass(BG) but a relatively low bone regeneration effect was seen. CONCLUSION: Good restorative results of the periodontal tissue can be attained by applying the various bone graft materials being used today after identifying the accurate clinical effects.
Allografts ; Alveolar Bone Loss ; Bone Regeneration ; Bone Transplantation ; Gingival Recession ; Heterografts ; Membranes ; Transplants*

The purpose of this study was to evaluate 6 years cumulative survival rate (CSR, %) of mandibular posterior single tooth implants replaced with Branemark TiUnite(R) implant system. The findings from this study were as followed ; 1. The 112 (111 persons) single implants that were placed in the mandibular posterior region were successful except 4 cases and showed 96.42% CSR. 2. The 55 (55 persons) single implants that were placed in the mandibular first molar region were successful except 2 cases and showed 96.36% survival rate. And, among the 57 (56 persons) single implants replacing the mandibular second molar, 2 failed showing 96.49% survival rate. There was no significant statistical difference. 3. Among the total 112 implants, 5.0mm wide diameter implants were placed in 96 cases(85.7%) showing 96.9% survival rate. 4.0mm standard diameter implants were placed in 16 cases showing 93.8% survival rate. There was no significant statistical difference. 4. Long implants above 10.0mm length were placed 103 cases(91.0%) and showed 96.1% survival rate. Short implants within 8.5mm length were placed 9 cases and showed 100% survival rate. There was no significant statistical difference. 5. 37 implants placed in typeI,II bone quality were showed higher survival rate(100%) than that of 52 implants placed in typeIII, IV bone quality(92.3%). But, there was no significant statistical difference. In conclusion, Branemark TiUnite(R) implant showed successful results when replacing manbibular single molar.
Dental Implants, Single-Tooth ; Molar ; Retrospective Studies* ; Survival Rate ; Tooth*