BACKGROUND: Various topical antisepties are often used for postoperative care of surgical wounds. But the effect of these agent on wound healing remains an area of debate. OBJECTIVE: The purpose of this study was to evaluate the biological effects of hydrogen peroxide on human keratinocytes in vitro, specifically on keratinocyte viability, migration and proliferation. METHODS: To evaluate the effects of hydrogen peroxide on keratinocyte viability, migration and proliferation, sulforhodamine B test, migration assay, and thymidine incorporation assay were performed, respectively. Diluted solutions of 30% hydrogen peroxide( X 50, X 100, X 500, X 1000) were used as testing material and growth medium alone as a control.
Humans* ; Hydrogen Peroxide* ; Hydrogen* ; Keratinocytes* ; Postoperative Care ; Thymidine ; Wound Healing* ; Wounds and Injuries*

No abstract available.

No abstract available.
Biliary Tract Diseases* ; Biliary Tract* ; Reoperation*

No abstract available.
Hemorrhage*

BACKGROUND: Laparoscopic cholecystectomy has become the standard treatment of benign gallbladder disease. It requires the skill of a trained surgeon, and its safe performance. The advantages of laparoscopy are less postoperative pain, absence of cosmetic damage, shorter recovery times, and decreased length of hospital stays compared with conventional open surgery. METHODS: We reviewed 500 consecutive laparoscopic cholecystectomies retrospectively at the department of general surgery of Maryknoll Hospital from February 1992 to May 1999. RESULTS: There were 175 males and 325 females(M:F=1:1.86) with ages ranged from 21 to 78 years (mean: 50.0 year). Preoperatively, 72 patients underwent endoscopic retrograde cholagiography and 7 patients underwent endoscopic sphincterotomy due to common bile duct stone. Mean operation time was 73.7 minutes. Operative cholangiogram was attempted in 8 patients. Conversion to open cholecystectomy during operation was done in 8 cases(1.6%) and leading causes were severe adhesion, uncontrolled bleeding, suspected malignancy, bile duct injury. Operative Complications occured in 18 patients(3.6%): bile duct injury 1, postoperative bleeding 1, bile leakage 4,subcutaneous emphysema 1, abdominal wall hematoma 3, wound infection 8. There was no operative mortality. The length of hospital stay ranged from 2 to 15 day with average of 4.7 day. CONCLUSION: Laparoscopic cholecystectomy is a safe and effective operation in patients with benign gallbladder diseases.
Abdominal Wall ; Bile ; Bile Ducts ; Cholecystectomy ; Cholecystectomy, Laparoscopic* ; Common Bile Duct ; Emphysema ; Gallbladder Diseases ; Hematoma ; Hemorrhage ; Humans ; Laparoscopy ; Length of Stay ; Male ; Mortality ; Pain, Postoperative ; Retrospective Studies ; Sphincterotomy, Endoscopic ; Wound Infection

No abstract available.
Thioacetamide*

No abstract available.
Lichens* ; Mucinosis, Follicular*

PURPOSE: Kidney transplantation is definite treatment in chronic renal failure (CRF). But CRF patients have contracted bladder due to disuse atrophy and fibrosis of bladder mucosa and muscle. Contracted bladder results in CRF itself and failure of transplantation. And this causes many difficulties in ureteroneocystostomy. So many authors suggest that preoperative bladder augmentations of contracted bladder (cystoplasty) using intestine increase success rate in kidney transplantation. But these methods have been usually studied in pediatric transplantation. Preoperative hydrostatic bladder dilatation is nonoperative treatment usually used in interstitial cystitis and hemorrhagic bladder tumor. METHODS: Since January 1996, we newly attempted pretransplant bladder augmentation using hydrostatic pressure in 22 CRF patients who had contracted bladder diagnosed through preoperative voiding cystourethrogram (VCUG). RESULTS: Pre-augmented average bladder volume was 87.7 mL (60~100 mL) and post-augmented bladder volume was 210.5 mL (100~250 mL). There was no complication associated with pretransplant bladder augmentation itself, and there was no transplanted kidney loss. CONCLUSION: These results suggest that pretransplant bladder augmentation using hydrostatic pressure be useful in kidney transplantation of CRF patient who had contracted bladder.
Cystitis, Interstitial ; Dilatation ; Fibrosis ; Humans ; Hydrostatic Pressure* ; Intestines ; Kidney ; Kidney Failure, Chronic* ; Kidney Transplantation ; Mucous Membrane ; Muscular Disorders, Atrophic ; Urinary Bladder Neoplasms ; Urinary Bladder*

The surgical technique of renal transplantation has been well established, but surgical complications are not uncommon and still be important factors in postoperative mortality and morbidity. From August 1990 to May 1998, we performed 250 consecutive renal transplantations at Maryknoll hospital. The authors analyzed the kinds and incidences of surgical complications in a single center by using information from hospital records. The results were as follows: 1. The overall surgical complication rate was 9.6% (24 episodes in 250 cases). 2. These were composed of 6 vascular, 8 urologic, 4 lymphatic, and 6 wound complications. 3. Vascular complication included renal artery stenosis in 1 case and bleeding and hematoma in 5 cases. 4. Urologic complication included urine leakage in 4 cases, ureter stenosis in 2 cases and bladder stone in 2 cases. 5. Lymphatic complication was all lymphoceles. 6. One case in which renal artery stenosis had occured progressed to graft loss after 4 years later. There was no complication related mortality. 7. 1 year, 3 year and 5 year graft survival rate was 97.2%, 94.8% and 90.0%, respectively. and patient survival rate was 98.0%, 95.6% and 94.7%, respectively.
Constriction, Pathologic ; Graft Survival ; Hematoma ; Hemorrhage ; Hospital Records ; Humans ; Incidence ; Kidney Transplantation ; Lymphocele ; Mortality ; Renal Artery Obstruction ; Survival Rate ; Transplants ; Ureter ; Urinary Bladder Calculi ; Wounds and Injuries

No abstract available.
Animals ; Linoleic Acid* ; Macrophages, Peritoneal* ; Mice*