PUROPOSE: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common disorder to men and the syndrome is diagnosed only on the basis of symptoms, principally pain or discomfort in the pelvic region. However many patients have a urinary symptoms, especially storage symptoms such as frequency and urgency. The aim of this study was to evaluate the efficacy of anticholinergics in a prospective randomized, single-blind trial to young and middle aged patients to exclude the effect of benign prostatic hyperplasia as possible. MATERIAL AND METHODS: Forty six men with CP/CPPS (age of third to fifth decades) were randomized in a single-blind fashion to receive either group 1; gatifloxacin (200mg, bid) of 15 patients, or group 2; gatifloxacin (200mg, bid) and propiverine (20mg, once daily) of 31 patients for 2 months. The NIH chronic prostatitis symptom index (NIH-CPSI) and International Prostate Symptom Score (IPSS) were used to grade symptoms and the quality of life (QoL) impact at the start, 1 month and 2 months of the study. RESULTS: There was no significant difference between group 1 and group 2 in about age and duration of the disease. In addition, no significant difference was found between group 1 and 2 in the scores of sub-factors of IPSS and NIH-CPSI at the time of baseline. No statistically significant difference in the NIH-CPSI total score and each domain was seen after treatment. Statistically significant difference in the storage symptom of IPSS was seen after treatment. CONCLUSIONS: The efficacy of propiverine (combined to antibiotics) for CP/CPPS were improvements of the storage symptom of IPSS significantly.
Cholinergic Antagonists ; Humans ; Male ; Middle Aged ; Pelvic Pain* ; Pelvis ; Prospective Studies ; Prostate ; Prostatic Hyperplasia ; Prostatitis* ; Quality of Life

PURPOSE: The aim of this study was to investigate the effects of testosterone deprivation on urinary bladder in male rabbits by proteomic analysis. MATERIALS AND METHODS: New Zealand white male rabbits (2.5-3 kg) were divided into 2 group; control group with 5 rabbits and bilateral orchiectomized group, bilateral orchiectomized group was divided into post-operative 4 weeks group (group 1), and 8 weeks group (group 2) with 5 rabbits respectively. Bladder wall was excised partly at 4 or 8 weeks from the beginning of the experiment. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry. We decided to consider 'significant' if protein had 50% decreasing or 200% increasing expression rate. RESULTS: Six proteins were significantly changed in orchiectomy group, compared to control group; serum albumin precursor, GABA transaminase, dimethylarginine dimethylaminohydrolase 2, serum/glucocorticoid regulated kinase and LOC304923 protein were over-expressed in both group 1 and group 2. Annexin A1 was significantly over-expressed in the group 2 only. CONCLUSION: An overexpression of Annexin A1 and GABA transaminase mean a processing of neuronal reactions to injury in orchiectomized rabbit bladder. Also an overexpression of dimethylarginine dimethylaminohydrolase 2 would increase NO synthesis and thereby promote re-endothelialization in the rabbit bladder after orchiectomy. Serum/glucocorticoid regulated kinase would relate to Na+ transport. Albumin precursor and LOC304923 protein are remained to further research. These data suggested that bilateral orchiectomy would make the urinary bladder unstable. However more information is needed in human bladder tissue.
4-Aminobutyrate Transaminase ; Annexin A1 ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Male ; Mass Spectrometry ; Neurons ; New Zealand ; Orchiectomy* ; Phosphotransferases ; Proteomics ; Rabbits ; Serum Albumin ; Testosterone ; Urinary Bladder*

No abstract available.
Nucleopolyhedrovirus* ; Sequence Analysis*

PURPOSE: 5alpha reductase, dutasteride, has widely used to treat enlarged prostate (BPH). By suppressing the conversion of testosterone to dihydrotestosterone it decreases serum prostate-specific antigen (PSA) which is very important screening marker for prostate cancer. We evaluate the early serum PSA changes after dutasteride treatment to Korean BPH patients. MATERIALS AND METHODS: A total 159 men with a clinical diagnosis of BPH and no evidence of prostate cancer were enrolled. They were treated with dutasteride 0.5mg daily for 12 months. Serum PSA was evaluated at 2, 6, and 12 months after the medication. RESULT: Dutasteride statistically significantly reduced serum PSA to 0.70+/-0.52, 0.64+/-0.35, and 0.59+/-0.49 from baseline level at 2, 6, and 12 months after the medication, respectively. However, there was no statistical significance among the three groups in serum PSA changes after dutasteride. There were statistically significant correlations between a high pre-treatment serum PSA level and a large reduction of follow-up PSA levels at 2, 6, and 12 months after dutasteride treatment. CONCLUSIONS: The reduction of serum PSA is variable in patients to patients at 2, 6, and 12 months after dutasteride treatment. The patient with high initial serum PSA revealed a large reduction of serum PSA level after treatment. The traditional concept that follow-up serum PSA level should be doubled for prostate cancer screening may overestimate real serum PSA level within 12 months in Korean men receiving 5alpha reductase inhibitors.
Diagnosis ; Dihydrotestosterone ; Follow-Up Studies ; Humans ; Hypertrophy* ; Male ; Mass Screening ; Oxidoreductases ; Prostate* ; Prostate-Specific Antigen* ; Prostatic Neoplasms ; Testosterone ; Dutasteride

The importance of vegetables as a allergen for the development of hand eczema has been a matter of debate. The aim of this study was to evaluate the clinical manifestations of hand eczema and to investigate the role of vegetables in the hand eczema of Korean housewives. In this study, clinical evaluatons and patch tests with Hollister-Stier standard allergens and 22 vegetable allergens wer performed in 32 patients with housewife's hand eczema. The results are summarized as follows : 1. The prevalence was highest in fourth(59.4%) and third(25.0%) decades of life and the peak age of onset was third(53.1%) and fourth(31.3%) decades. 2. Only 3 of 32 patients(9.4%) had past histories of atopic dermatitis ; however, childrens of 12 patients(37.5%) had atopic dermatitis. 3. The common skin signs were fissuring(90.6%), scaling(78.1%), erythema(75.0%), hyperkeratosis(71.9%), lichenification(62.5%), and vesicle(43.8%) in descending order. 4. The common aggravating factors were soap or detergent(93.8%), water(90.6%) and rubber glove(25.0%). 5. The most common involved areas of hand eczema were fingers including fingertips and fingertips only. 6. The results of patch test using vegetable allergens with 10 normal controls were all negative. Of the 32 patients with hand eczema, 13(37.5%) showed positive patch test with standard allergens and 5(15.6%) showed positive with vegetable allergens. 7. Among 30 standard allergens, cinnamic alcohol(9.4%). potassium dichromate(9.4%), nickel sulfate(6.3%), and wool wax alcohol(6.3%) were the common allergens which showed positive reactions. Of the 22 vegetable allergens, garlic, Chinese cabbage juice, Chinese cabbage extract, cucumber juice, spinach extract, potato juice showed weak positive reactions in only one patient(3.1%) respectively. From the above results contact sensitivity to vegetables is not significant in Korean housewives with hand eczema.
Age of Onset ; Allergens ; Asian Continental Ancestry Group ; Brassica ; Child ; Dermatitis, Atopic ; Dermatitis, Contact ; Eczema* ; Fingers ; Garlic ; Hand ; Humans ; Nickel ; Patch Tests* ; Potassium ; Prevalence ; Rubber ; Skin ; Soaps ; Solanum tuberosum ; Spinacia oleracea ; Vegetables* ; Wool

Acute cerebral infarctions are rare in children, however, they can occur as a complication of a Mycoplasma pneumoniae (MP) infection due to direct invasion, vasculitis, or a hypercoagulable state. We report on the case of a 5-year-old boy who had an extensive stroke in multiple cerebrovascular territories 10 days after the diagnosis of MP infection. Based on the suspicion that the cerebral infarction was associated with a macrolide-resistant MP infection, the patient was treated with levofloxacin, methyl-prednisolone, intravenous immunoglobulin, and enoxaparin. Despite this medical management, cerebral vascular narrowing progressed and a decompressive craniectomy became necessary for the patient's survival. According to laboratory tests, brain magnetic resonance imaging, and clinical manifestations, the cerebral infarction in this case appeared to be due to the combined effects of hypercoagulability and cytokineinduced vascular inflammation.
Brain ; Cerebral Infarction* ; Child ; Child, Preschool ; Decompressive Craniectomy ; Diagnosis ; Enoxaparin ; Humans ; Immunoglobulins ; Inflammation ; Levofloxacin ; Magnetic Resonance Imaging ; Male ; Mycoplasma pneumoniae* ; Mycoplasma* ; Pneumonia, Mycoplasma* ; Stroke ; Thrombophilia ; Thrombosis ; Vasculitis

Purpose: The study aimed to examine whether prophylactic surfactant replacement therapy (SRT) with less invasive surfactant administration (LISA) by tracheal catheterization in a group of spontaneously breathing preterm infants would improve clinical outcomes compared to prophylactic SRT with the INtubation-SURfactantExtubation (INSURE) method. Methods: We compared 20 spontaneously breathing preterm infants, 25 to 29 weeks of gestation or with a birth weight of less than 1,250 g, treated with prophylactic SRT using a gastric tube (LISA group), to the 20 spontaneously breathing preterm infants matched by gestational age and birth weight, managed with prophylactic SRT via the INSURE method (INSURE group, historical control). Results: The LISA group had lower rates of mechanical ventilation (MV) 72 hours after birth (P=0.019) and at any time (P=0.025), lower frequency of bradycardia during SRT (P=0.031), and lower median duration of MV than the INSURE group (P=0.038). In multivariate analysis, the LISA method was associated with a significantly lower likelihood of receiving invasive ventilation during hospitalization (odds ratio [OR], 0.029; 95% confidence interval [CI], 0.001 to 0.938; P=0.046) and a decreased frequency of bradycardia during SRT (OR, 0.020; 95% CI, 0.001 to 0.535; P=0.020) as compared to the INSURE method. Conclusion Prophylactic SRT using LISA via tracheal catheterization in preterm infants may significantly reduce exposure to MV during hospitalization and bradycardia during surfactant administration.

PURPOSE: The pathophysiological role of androgen deprivation in male sexual dysfunction remains controversial, and this is especially true at the molecular level. We investigated the effect of androgen deprivation on the changes of proteins in the penile corpus cavernosum of castrated rabbits by the proteomic approach. MATERIALS AND METHODS: New Zealand white male rabbits(2.5-3kg) were divided into 2 groups: the control group with 5 rabbits and the bilateral orchiectomized group. The bilateral orchiectomized group was divided into the post-operative 4 weeks group(group 1), and the 8 weeks group (group 2) with 5 rabbits in groups 1 and 2, respectively. The penile corpus cavernosum was partly excised at 4 or 8 weeks from the beginning of the experiment. Conventional proteomics was performed with high resolution 2-D gel electrophoresis; this was followed by computational image analysis and protein identification with using mass spectrometry. RESULTS: A comparison of the corpus cavernosum of the orchiectomized group with the control group showed that nine proteins had a changed expression. Glycerol-3-phosphate dehydrogenase and F-actin binding protein were under-expressed in groups 1 and 2, and myosin regulatory light chain 2, tropomyosin beta chain and tropomyosin 1 were significantly under- expressed in group 1 and they were insignificantly over-expressed in group 2. In addition there were 4 proteins that were insignificantly under- expressed; skeletal muscle myosin heavy chain MyHC-EO/III, a protein that was similar to suppressor of cytokine signaling 6 isoform, myosin light chain 1 and heat shock 27kDa protein 1. CONCLUSIONS: This data suggests that changes of proteins, and especially tropomyosin 1, mean there is processing of the cellular apoptosis pathway in the orchiectomized rabbits' corpus cavernosum. However more information is needed about human corpus cavernosal tissue.
Male ; Humans ; Rabbits ; Animals

PURPOSE: The pathophysiological role of androgen deprivation in male sexual dysfunction remains controversial, and this is especially true at the molecular level. We investigated the effect of androgen deprivation on the changes of proteins in the penile corpus cavernosum of castrated rabbits by the proteomic approach. MATERIALS AND METHODS: New Zealand white male rabbits(2.5-3kg) were divided into 2 groups: the control group with 5 rabbits and the bilateral orchiectomized group. The bilateral orchiectomized group was divided into the post-operative 4 weeks group(group 1), and the 8 weeks group (group 2) with 5 rabbits in groups 1 and 2, respectively. The penile corpus cavernosum was partly excised at 4 or 8 weeks from the beginning of the experiment. Conventional proteomics was performed with high resolution 2-D gel electrophoresis; this was followed by computational image analysis and protein identification with using mass spectrometry. RESULTS: A comparison of the corpus cavernosum of the orchiectomized group with the control group showed that nine proteins had a changed expression. Glycerol-3-phosphate dehydrogenase and F-actin binding protein were under-expressed in groups 1 and 2, and myosin regulatory light chain 2, tropomyosin beta chain and tropomyosin 1 were significantly under- expressed in group 1 and they were insignificantly over-expressed in group 2. In addition there were 4 proteins that were insignificantly under- expressed; skeletal muscle myosin heavy chain MyHC-EO/III, a protein that was similar to suppressor of cytokine signaling 6 isoform, myosin light chain 1 and heat shock 27kDa protein 1. CONCLUSIONS: This data suggests that changes of proteins, and especially tropomyosin 1, mean there is processing of the cellular apoptosis pathway in the orchiectomized rabbits' corpus cavernosum. However more information is needed about human corpus cavernosal tissue.
Male ; Humans ; Rabbits ; Animals

No abstract available.
Child* ; Hepatitis B, Chronic* ; Hepatitis, Chronic* ; Humans ; Interferon-alpha*