1.Studies on Identification and Drug Resistance of Atypical Mycobacteria Isolated from Patients with Pulmonary Tuberculosis.
Dong Hyun CHUNG ; Sung Kwang KIM ; Joo Deuk KIM
Yeungnam University Journal of Medicine 1984;1(1):49-58
The differential diagnosis of atypical mycobacteriosis caused by atypical mycobacteria (with the exception of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium leprae) which are widly distributed in soil and water, from pulmonary tuberculosis is possible only when atypical mycobacteria are isolated and identified. In this investigation, attempts were made to isolate atypical mycobacteria from persons registered as tuberculosis patients in the Anyang Health Center in Anyang City, Kyungki province, Korea. Biological and biochemical tests were performed for the atypical mycobacteria isolated from these patients, also retrospective analysis of clinical and X-ray findings of the patients with bacteriologically confirmed atypical mycobacteriosis were done. The results can be summarized as follows; 1. 103 strains of mycobacteria were isolated among 334 sputum samples from patients. 2. Among the isolated mycobacteria, 10 strains (9.7%) were found to be an atypical mycobacteria and 93 strains (90.3%) were tubercle bacilli of human type. 3. On the basis of Runyon's grouping of atypical mycobacteria, there were 3 strains (30.0%) of scotochromogen and nonphotochromogen respectively, 4 strains (40.0%) of rapid grower, and no photochromogen. 4. By biochemical tests, 3 strains of scotochromogen were identified as Mycobacterium scroful-aceum (2 strains) and Mycobacterium szulgai (1 strain) 3 strains of nonphotochromogen were Mycobacterium avium-complex (2 strains) and Mycobacterium terriae (1 strain), and 4 strains of rapid grower were Mycobacterium fortuitum (3 strains) and Mycobacterium chelonae. 5. In drug sensitivity tests, all 10 strains isolated atypical mycobacteria showed resistance to various concentration of INH and SM and low concentration (10 mcg, 40 mcg and 50 mcg) of EB, TH, and CS, and were sensitive to only high concentration (20 mcg and 100 mcg) of EB, TH, CS, and RFP. 6. In analysis of clinical findings by the patients with bacteriologically confirmed atypical mycobacteriosis, it was found that clinical symptoms of these patients appeared not to be mild than those of patients with pulmonary tuberculosis. The patients with atypical mycobacteriosis had been treated for pulmonary tuberculosis for a long time and they showed no improvement.
Diagnosis, Differential
;
Drug Resistance*
;
Gyeonggi-do
;
Humans
;
Korea
;
Mycobacterium
;
Mycobacterium bovis
;
Mycobacterium chelonae
;
Mycobacterium fortuitum
;
Mycobacterium tuberculosis
;
Nontuberculous Mycobacteria*
;
Retrospective Studies
;
Soil
;
Sputum
;
Tuberculosis
;
Tuberculosis, Pulmonary*
;
Water
2.Expression of the 38 kDa Protein of Mycobacterium tuberculosis in M . bovis BCG and Use in the Serodiagnosis of Tuberculosis.
Sang Nae CHO ; Hee Jin KIM ; Hye Young LEE ; Seung Chul KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1999;34(6):555-559
The 38 kDa protein of Mycobacterium tuberculosis, which was known previously as antigen 5, has been extensively used in the serodiagnosis of tuberculosis. In an attempt to develop and evaluate a serodiagnostic test using the antigen, we expressed the 38 kDa protein in BCG and its seroreactivity was compared to that expressed in Escherichia coli. The coding region of the 38 kDa protein was amplified by PCR, and the gene was cloned into a Mycobacterium-E. coli shuttle expression vector pYMC-his and pQE30 expression vector and expressed in BCG and E. coli, respectively. Both recombinant 38 kDa proteins showed strong seroreactivity against pooled serum from tuberculosis patients. There was no significant difference in seroreactivity between the two recombinant antigens in sera from the far advanced tuberculosis patients. However, of 25 tuberculosis patients graded as ""minimal"" by chest X-ray, 5 (20.0%) were seropositive by r38 kDa expressed in E. coli, while 8 (32.0%) by that expressed in BCG. Likewise, higher seroreactivity by r38 kDa expressed in BCG was found in sera from the moderately advanced tuberculosis. This study thus indicates that the recombinant 38 kDa expressed in BCG is more effective than that expressed in E. coli in detecting antibodies to the native 38 kDa protein of M. tuberculosis in sera from minimally affected tuberculosis patients.
Antibodies
;
Clinical Coding
;
Clone Cells
;
Escherichia coli
;
Humans
;
Mycobacterium bovis*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymerase Chain Reaction
;
Serologic Tests*
;
Thorax
;
Tuberculosis*
3.The HLA Antigen and Leprosy in Korea.
Se Jong KIM ; In Hong CHOI ; Joo Deuk KIM
Yonsei Medical Journal 1985;26(2):154-158
To investigate the genetic factors in Koreans with leprosy, 157 unrelated leprosy patients have been typed for HLA antigens, and compared with 162 healthy controls. The patient group consisted of 124 with lepromatous leprosy and 33 with tuberculoid leprosy. HLA-A11 was found to be increased in lepromatous leprosy (p=0.0005). HLA-Aw33 was found to be increased in both lepromatous leprosy (p = 0.0002) and tuberculoid leprosy (p = 0.005). HLA-Cw5 was found to be decreased in lepromatous leprosy (p = 0.009). Frequencied of HLA-B antigens did not differ significantly between the leprosy patients and the healthy controls.
HLA Antigens/analysis*
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Human
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Korea
;
Leprosy/genetics
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Leprosy/immunology*
;
Phenotype
4.HLA-linked Genetic Markers in Koreans.
Joo Deuk KIM ; In Hong CHOI ; Se Jong KIM
Yonsei Medical Journal 1983;24(2):127-131
The polymorphic variants of HLA-linked genetic markers GLO, Bf, C2 and C4 were determined in Koreans. The GLO2 allele was found at a similar frequency as compared with other orientals and at higher frequency than in Caucasians. The gene frequencies of BfS and BfF showed different figures from those in other orientals and were similar to Caucasians. The C2C allele was the highest variant as in other populations. The rare variant C2A was not observed in this study. The common variants of C4A alleles are C4A*3 and C4A*4. Among the C4B variants, C4B*1, C4B*2 and C4B* QO are common in that order. Several undefined electrophoretic variants C4A and C4B were observed in this study. These findings suggest that the frequencies of various HLA-linked genetic markers can be used in anthropological studies.
Caucasoid Race
;
Comparative Study
;
Genetic Markers*
;
HLA Antigens/genetics*
;
Human
;
Korea
;
Mongoloid Race*
;
Polymorphism (Genetics)
5.Diversity of Humoral Immune Responses to Recombinant Proteins of Brucella abortus Among Residents in Cheju Province.
Hyung Jin EUH ; Jun Seop YEOM ; Jun Myung KIM ; Joo Deuk KIM ; Sang Nae CHO
Journal of the Korean Society for Microbiology 2000;35(5):377-377
No Abstract Available.
Brucella abortus*
;
Brucella*
;
Immunity, Humoral*
;
Jeju-do*
;
Recombinant Proteins*
6.Immunoblotting analysis of antibodies against borrelia burgdorferi, the lyme disease agent, in sera from the Korean residents.
Sang Nae CHO ; Tae Yoon LEE ; Mi Kyeong LEE ; Dook Soon KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1991;26(3):263-272
No abstract available.
Antibodies*
;
Borrelia burgdorferi*
;
Borrelia*
;
Immunoblotting*
;
Lyme Disease*
7.Construction of a Mycobacterium - Escherichia coli Shuttle Vector and Use in the Expression of Foreign Genes in Mycobacteria.
Sang Nae CHO ; Joo Deuk KIM ; Hye Young LEE ; Hee Jin KIM
Journal of the Korean Society for Microbiology 1997;32(3):293-300
The ability to introduce recombinant DNA molecules back into mycobacteria would greatly increase the potential of molecular genetic approaches for the study of mycobacteria as well as for the use in clinical purposes. We have initiated the construction of vectors that facilitates the introduction of recombinant DNA into mycobacteria. The vector was designed to contain replicons for multiplication in mycobacteria and Escherichia coli, a promoter for gene expression, a drug resistant gene for selecting transformants, and a few restriction enzyme sites for convenient cloning. Constructed Mycobacterium-E. coli shuttle vector named p YMC (hsp60) was shown to transform M. smegmatis at high efficiency and maintain plasmid at stable level. The ability of the vector to express cloned foreign gene was also monitored by measuring the expressed level of luciferase gene which was used as a reporter. High level of luciferase activity in M. smegmatis with pYMC (hsp60:luc) was detected confirming successful construction of Mycobacterium-E. coli shuttle vector.
Clone Cells
;
Cloning, Organism
;
DNA, Recombinant
;
Escherichia coli*
;
Escherichia*
;
Gene Expression
;
Genetic Vectors*
;
Luciferases
;
Molecular Biology
;
Mycobacterium*
;
Plasmids
;
Replicon
8.Identification of the CDR3 Gene Sequence on the beta Chain of the T Cell Receptor in T Cell Leukemia Cell Line.
In Hong CHOI ; Se Jong KIM ; Joo Deuk KIM ; Yong Joon CHWAE
Korean Journal of Immunology 1997;19(4):541-548
In order to develop a method for the detection of minimal residual leukemic disease (MRD) in T cell acute lymphocytic leukemia (T cell ALL), T cell leukemia cell line was used to detect clonal TCR p chain mRNA and to synthesize CDR3 specific oligonucleotide probe. For the Jurkat cell line clonal TCR p chain cDNA was amplified by using RT-PCR with oligonucleotide primer, Vp universal primer. As the result of RT-PCR an approximate 300 bp fragment of the TCR chain was obtained, and the partial identification of the TCR p chain gene and the amino acid sequence of the fragment were done by gene cloning and sequencing. The gene sequence of TCR p obtained was identified as Vp8-Jp1.2-Cp2. Diversity gene segment could not be found. Within the p chain, the CDR3 region was identified as 12 amino acids (SFSTCSANYGYT). It is kown that TCR is expressed in about 40% of the all T cell ALL. However it is not kown what percentage of the TCR p chain mRNA expression translates into the actual TCR molecule. It is not certain how many patients with MRD can be detected by this method used in this study, but this technique might be useful to detect MRD in at least 40% of the patients with T-cell ALL.
Amino Acid Sequence
;
Amino Acids
;
Cell Line*
;
Clone Cells
;
Cloning, Organism
;
DNA, Complementary
;
Humans
;
Jurkat Cells
;
Leukemia, T-Cell*
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
Receptors, Antigen, T-Cell*
;
RNA, Messenger
;
T-Lymphocytes
9.Circulating Immune Complexes in Patients with Leprosy.
Joo Young PARK ; Kyung Won KIM ; Se Jong KIM ; Joo Deuk KIM
Yonsei Medical Journal 1984;25(1):18-26
The occurrence of immune complexes in the serum from rats infected with M. leprae-murium and 38 patients with leprosy were studied by the polyethylene glycol precipitation complement consumption (PEG-CC) test and the results were compared in the various forms of the disease. Circulating immune complexes (CIC) were significantly increased in the sera from rats infected with M. lepraemurium compared to normal control rats (P < 0.005). There were no significant differences between the the level of CIC in the sera from lepromatous leprosy patients and that from tuberculoid leprosy patients, but in the sera from patients with erythema nodosum leprosum (ENL) the level of CIC was significantly increased (P < 0.005). And although we couldn't find a corre1ation between the level of CIC and bacterial indices in lepromatous leprosy patients, CIC tends to de-crease after negative conversion of their bacterial indices. These findings suggested that the detection of CIC can be of some practical interest in the early diagnosis of ENL and can be a valuable assessment in following the therapy after negative conversion of their bacterial indices.
Animal
;
Antigen-Antibody Complex/analysis*
;
Disease Models, Animal
;
Human
;
Leprosy/immunology*
;
Male
;
Rats
;
Reference Values
;
Time Factors
10.Recent Trends of Syphilis Incidence in Korea.
Yonsei Medical Journal 1968;9(1):74-80