1.Dioscorea japonica Thunb. Ethanolic Extract Attenuated Oxazolone-Induced Atopic Dermatitis-like Skin Lesions in BALB/c Mice
Jonghwan JEGAL ; No June PARK ; Beom Geun JO ; Su Nam KIM ; Min Hye YANG
Natural Product Sciences 2019;25(3):261-267
The rhizomes of Dioscorea japonica Thunb. are widely consumed as food and also used to treat diabetes and polyuria in Korea. This study was undertaken to study the anti-atopic dermatitis effects of a 95% ethanolic extract (DJE) of D. japonica in an oxazolone-stimulated murine model of atopic dermatitis (AD). The therapeutic effects of DJE on AD-like skin lesions were assessed on both ears. DJE (1%) or dexamethasone (0.5%; the positive control) were applied to skin lesions for three weeks. Serum levels of IgE and IL-4 were assessed by ELISA (enzyme-linked immunosorbent assay). Histopathological examinations were performed by hematoxylin and eosin (H&E) and toluidine blue staining and revealed DJE significantly reduced dermal thickness and inflammatory cell infiltration when applied to oxazolone-treated ear skin. DJE-treated AD mice also showed lower serum levels of IgE and IL-4 than oxazolone-stimulated controls. Our findings demonstrate DJE might be a useful safe, topical agent for the treatment of atopic diseases.
Animals
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Dermatitis
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Dermatitis, Atopic
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Dexamethasone
;
Dioscorea
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Ear
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Enzyme-Linked Immunosorbent Assay
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Eosine Yellowish-(YS)
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Ethanol
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Hematoxylin
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Immunoglobulin E
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Interleukin-4
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Korea
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Mice
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Oxazolone
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Polyuria
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Rhizome
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Skin
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Therapeutic Uses
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Tolonium Chloride