After the synthesis of IL-15 cDNA from the total RNA of mouse spleen, it was inserted into the prokaryotic expression vector, pRseta, and eukaryotic expression vector, pcDNA3.0, respectively. Subsequently, the insertion of gene and open reading frame were confirmed by sequencing of each plasmid, respectively. Using pRseta- IL-15 plasmid, the recombinant IL-15 protein was induced by IPTG under BL21 (DE 3) host cells and recombinant IL-15 was expressed at 14.5 KDa with time. Then, IL- 15 was separated by His-tag affinity chromatography and analyzed by SDS-PAGE to yield soluble IL-15 at 14.5 KDa as monomer and 29.0 KDa as dimer. In order to inspect the function and contribution of IL-15, the in vitro experiment was established using mononuclear cells separated from the mouse spleen. After 48h exposure of PHA to mouse splenocyte and 24h treatment with recombinant IL-15, the effects of cytokine inductions inspected against IL-2, IL-6, IL-10, IL-12, IFN-r, and GM-CSF. The results showed that comparing with the control, IL-6 increased, IL-2, IL-12 and IFN-r increased and similar, and GM-CSF decreased. In addition, the direct injection of pcDNA3.0-IL-15 plasmid into mice gave the similar results to in vitro studies. Namely, IL-6 and IL-12 increased, and IL-2, IFN-r and GM-CSF were similar or decreased. IL-10 was not induced in in vitro and in vivo experiments. These results suggested that the IL-15 induce the splenocyte activation and can be an important factor in proliferation and fuction recovery of weakened T-cell.
Animals ; Chromatography, Affinity ; DNA, Complementary ; Electrophoresis, Polyacrylamide Gel ; Granulocyte-Macrophage Colony-Stimulating Factor ; Interleukin-10 ; Interleukin-12 ; Interleukin-15* ; Interleukin-2 ; Interleukin-6 ; Isopropyl Thiogalactoside ; Mice* ; Open Reading Frames ; Plasmids ; RNA ; Spleen ; T-Lymphocytes

Restoration of functional and anatomic changes of kidney in UPJ stricture is one of most important subjects in urology. A clinical observation was made on the 28 cases (29 kidneys) of pyeloplasties for UPJ stricture from Jan. 1976 to Dec. 1981. Of them 20 cases (21 kidneys) were followed up for more than 6 months postoperatively. The following results were obtained; 1. The patients in this series were 22 males and 6 females, and 11 children and 17 adults. The left side was 3 times more frequently affected than the right. They symptoms on admission were mostly flank pain, palpable mass, and etc. 2. Preoperative status of UPJ was; UPJ stricture alone in 20, UPJ stricture with aberrant vessel in 4, UPJ stricture with stone in 3, and UPJ stricture with vesicoureteral reflux in 1 case. 3. Preoperative grade of hydronephrosis was closely related to each other in the functional and anatomic aspects of kidney. In general, children showed relatively more advanced hydronephrosis than adults and the degree of improvement of hydronephrosis postoperatively was similar to each other. 4. Anderson Hynes' method of pyeloplasty in most cases (20 kidneys) and simple pyeloureterostomy in 9 kidneys were performed, and results between both operations showed no difference. But Anderson Hynes' method was more effective in marked hydronephrosis such as giant hydronephrosis and simple pyeloureterostomy in mild hydronephrosis. 5. In cases in which UTI was present preoperatively, postoperative UTI tended more to persist than in those not complicated with preoperative UTI, and in both sides postoperative UTI mostly persisted for 2 months and disappeared within 6 months. 6. The period of nephrostomy and ureteral stent was not directly related to postoperative results, but nephrostomy without ureteral stent relatively showed good results. 7. Postoperative complication was UTI in 2, re-do pyeloplasty in 1 and secondary nephrectomy in 1 case. 8. Improvement Of hydronephrosis continued for about a year after operation. It is recommended that more careful attention should be paid for the first year and be continued to watch for consecutive 2 years.
Adult ; Child ; Constriction, Pathologic* ; Female ; Flank Pain ; Humans ; Hydronephrosis ; Kidney ; Male ; Nephrectomy ; Postoperative Complications ; Prognosis* ; Stents ; Ureter ; Urology ; Vesico-Ureteral Reflux

Dendritic cell (DC)s are protessional antigen presenting cells and they have been used for antitumor immunotherapy or cell vaccines. However therapy using DC is restricted because the number of DC available from tissue is very low. Flt3 ligand (FL) has been known as a hematopoietic growth factor that increases proliferation of hematopoietic stem cells and progenitor cells, and recently it showed inducibility of dendritic cell (DC)s and signiticant antitumor effects in vivo. Thus FL will be frequently used for DC induction and antitumor immunotherapy in future. Here we constructed FL plasmid and studied its in vivo effect. FL plasmids were made by cloning of partial FL cDNA into pcDNA3 plasmid, and gene expression and protein producibility of FL plasmid were confirmed in Renca cells transfected with FL plasmid. Mice were injected with FL plasmid (100ug/mouse) three times and 20 days later mouse spleens were harvested for staining and RT-PCR. There were lots of blastogenic cells in the spleen of mice treated with FL plasmid. FL plasmid also induced DEC205, IL-12 and GM-CSF gene expression in mouse splenocyte. All these data suggest FL plasmid may be used for induction of DC and antitumor therapy as DNA adjuvant.
Mice ; Animals

Clear cell hidradenoma, generally as an eccrine sweat gland origin, is a fairly uncommon tumor and occurs as a slowly growing, usually solitary nodule. The histological patterns vary from one tumor to another and in different parts of the same tumor. We experienced three cases of clear cell hidradenoma which were diagnosed by the histopathologic examination of the tumor mass removed by surgical excision. Clinical and histopathologic features of each case were reviewed and compared.
Acrospiroma* ; Sweat Glands

This study was designed to investigate the mole- cular mechanism of chemokine induction by lipopoly-saccharide(LPS) of E. coli. Chemokine gene expression was evaluated by the reverse transcriptase-polymerase chain reaction(RT-PCR) assay using RNAs isolated either from kidneys of LPS-injected inice or from the mesangial cells stimulated with LPS, IFN-r or TNF-a. LPS was shown to induce interferon gamma(IFN- 7 ) inducible protein 10 (IP-10) and monokine induced by interferon gamma (MIG) in kidney. IP-10 gene expression was induced by LPS and IFN-r, but MIG gene expression was induced by IFN-r in mesangial cell. Chemokines induced by LPS increased splenocyte migration. Sodium salicylate, wortmanin and piperazine blocked LPS mediated chemokine induction suggesting the activation of nuclear factor-a B pathway. It is concluded from this study that mesangial cells are the target of LPS in the renal failure resulting from the systemic infections. LPS induces chemokines directly and/or indirectly in the mesan- gial cells, and these chemokines may associated with renal inflammation.
Chemokines ; Gene Expression* ; Inflammation ; Interferons ; Kidney ; Mesangial Cells* ; Renal Insufficiency ; RNA ; Sodium Salicylate

We herein present a case of simultaneous occurrence of apocrine adenocarcinoma, syringocystadenoma papilliferum, syringoma, and eccrine hydrocystoma arising in an organoid nevus (nevus sebaceus of Jadassohn) which had been present on the right occipitoparietal scalp of a 60-year-old man since birth.
Adenocarcinoma* ; Humans ; Middle Aged ; Nevus* ; Organoids* ; Parturition ; Scalp ; Skin* ; Syringoma

The purpose of this study is to investigate the effect of job stress and social support on the organizational effectiveness of hospital employees and to examine the role of social support in the experience of job stress among the employees. Previous studies have yielded mixed results regarding the role of social support. Some studies provide supporting evidence for the buffering effect of social support, while others do not. Still others report findings about reverse buffering effects. These inconsistent findings are, in part, accounted for by methodological problems such as poor measurement, small sample size, and the existence of high multicollinearity. To examine more rigorously the role of social support in relation to the negative effects of job stress, this study was carefully designed to overcome methodolgical shortcomings found in the past research. In addition, unlike the previous studies, which were concerned mostly with health-related variables as consequences of job stress, in this study, three work-related variables (job satisfaction, organizational commitment, and intent to stay) which had close relationships with organizational effectiveness were examined as output variables. The sample used in this study consisted of 353 employees from a university hospital in the suburbs of seoul. Data were collected with self-administered questionnaires and analyzed using canonical analysis and hierarchical regression analysis. The results of this study indicate that; (l) job stress has negative main effects on job satisfaction, organizational commitment, and intent to stay; (2) social support has positive main effects on the same three output variables, (3) social support does not moderate the harmful effects of job stress on the three outcome variables, and (4) the three-way interaction effects of (social support * job stress * gender) and of (social support * job stress * education) are not supported The implications of these findings for the management of human resources are discussed.
Humans ; Job Satisfaction ; Surveys and Questionnaires ; Sample Size ; Seoul

No abstract available.
Neurilemmoma* ; Stomach*

No abstract available.
Parotid Gland*

Alkaline Phosphatase ; Animals ; Biological Assay ; Biological Factors ; Bone Matrix ; Bone Morphogenetic Proteins ; Calcium ; Cartilage ; Chromatography ; Collagen ; Collagen Type I ; Electrophoresis, Polyacrylamide Gel ; Foreign Bodies ; Guanidine ; Osteogenesis ; Rats ; Sodium ; Tail ; Transforming Growth Factors