No abstract available.

Developmental surface ultrastructure of Macroorchis spinulosus was studied by scanning electron microscopy. One-day-old juvenile fluke was leaf-shaped and bent ventrally. Body surface was covered densely with peg-like spines and with cobblestone-like cytoplasmic processes. Ciliated sensory papillae were concentrated around oral sucker. Several unciliated sensory papillae occurred equidistantly on oral sucker and acetabulum. The ciliated papillae appeared in two longitudinal lines symmetric bilaterally on dorsal surface. On adult flukes, tegumantal spines became wider in middle of the body surface. The cytoplasmic processes differentiated into more fine velvety form. It is likely that the differentiated fine cytoplasmic processes are an increased absorptive surface to adult M. spinulosus. It is suggested that single pointed tegumental spines on anterior half of the body may be supportive for this fluke to migration.
Animals ; Microscopy, Electron, Scanning ; Rats ; Rats, Sprague-Dawley ; Research Support, Non-U.S. Gov't ; Trematoda/*anatomy & histology/*ultrastructure

While scaphoid injuries are the most common carpal bone injuries, injuries to other carpal bones also occur frequently, accounting for about 40% of all carpal bone injuries. These non-scaphoid injuries are often complex, typically resulting from high-energy trauma and involving damage to two or more carpal bones or surrounding soft tissues. The carpus is a complex joint with eight carpal bones, making an accurate initial diagnosis challenging in many cases. A missed diagnosis or delayed treatment can lead to complications such as malunion, nonunion, avascular necrosis, and carpal instability. These complications can result in arthritis, neurovascular compression, and tendon rupture, causing chronic pain and functional impairment of the wrist joint. Therefore, careful attention to diagnosis and treatment is essential at the time of injury.

While scaphoid injuries are the most common carpal bone injuries, injuries to other carpal bones also occur frequently, accounting for about 40% of all carpal bone injuries. These non-scaphoid injuries are often complex, typically resulting from high-energy trauma and involving damage to two or more carpal bones or surrounding soft tissues. The carpus is a complex joint with eight carpal bones, making an accurate initial diagnosis challenging in many cases. A missed diagnosis or delayed treatment can lead to complications such as malunion, nonunion, avascular necrosis, and carpal instability. These complications can result in arthritis, neurovascular compression, and tendon rupture, causing chronic pain and functional impairment of the wrist joint. Therefore, careful attention to diagnosis and treatment is essential at the time of injury.

While scaphoid injuries are the most common carpal bone injuries, injuries to other carpal bones also occur frequently, accounting for about 40% of all carpal bone injuries. These non-scaphoid injuries are often complex, typically resulting from high-energy trauma and involving damage to two or more carpal bones or surrounding soft tissues. The carpus is a complex joint with eight carpal bones, making an accurate initial diagnosis challenging in many cases. A missed diagnosis or delayed treatment can lead to complications such as malunion, nonunion, avascular necrosis, and carpal instability. These complications can result in arthritis, neurovascular compression, and tendon rupture, causing chronic pain and functional impairment of the wrist joint. Therefore, careful attention to diagnosis and treatment is essential at the time of injury.

PURPOSE: Refractory status epilepticus refers to patients who do not respond properly to adequate first-line drug treatment such as diazepam, phenytoin, phenobaribital, lorazepam and show longer than 60 minute seizure. This study was designed to determine the efficacy and safety of midazolam given as a continuous infusion in the treatment of status epilepticus in children. METHODS: Midazolam was infused to twelve children with seizures, for whom two time repeated doses of 0.3mg/kg of diazepam, 20mg/kg of phenobarbital, and 20mg/kg of phenytoin failed to bring the episode under control. All patients received a bolus of midazolam(0.15mg/kg) followed by a continuous infusion at 1microgram/kg/min. The dose was increased every 15 min until the episode of seizure was brought under control. Time required to control seizures, infusion rate, and side-effects were monitored. RESULTS: The mean age of the patient population was 6.06 yrs(range 2 months to 16 yrs; 6 females and 6 males). In 11 patients, seizures were controlled in a mean time of 60.1 min(range 15-180 min). The mean infusion rate was 8.5microgram/kg/min(range 1-20). The total treatment duration was 17.6 days(range 1-54 days). One patient who was confirmed to have cortical dysplasia failed to respond. Two of the patients showed respiratory depression and bradycardia. CONCLUSION: Midazolam is an effective and safe drug to control refractory seizures in children with status epilepticus.
Bradycardia ; Child* ; Diazepam ; Female ; Humans ; Lorazepam ; Malformations of Cortical Development ; Midazolam* ; Phenobarbital ; Phenytoin ; Respiratory Insufficiency ; Seizures ; Status Epilepticus*

A case of multiple hamartomas of the unilateral chest wall in a four month old infant is presented. There have been a few reports on the CT findings of the chest wall hamartoma in infancy. We describe bone changes of the ribs and mineralization of this rare tumor on the CT scan, and the locations of two separate masses.
Hamartoma ; Humans ; Infant ; Miners ; Ribs ; Thoracic Wall* ; Thorax* ; Tomography, X-Ray Computed

PURPOSE: To evaluate differential points of clustered microcalcification in malignant and benign lesions on histopathology and correlate with mammographic findings. MATERIALS AND METHODS: Authors evaluated differential points of malignant and benign calcifications in histopathologically confirmed 24 cases. In two patients, mapping studies and paraffin block mammographic examinations were done to correlate calcification on mammography with histopathologic morphology. RESULT: On histopathology, malignant calcifications were located in ductal lumen, while benign ones were usually located in ductal epithelium(p<0.05). However, associated three benign lesions of the 10 malignant lesions showed still benign calcifications adjacent to proven cancer. The typical malignant calcifications were the shape of lamination, while benign calcifications showed granular and salty on light microscope. In 2 mapping studies, malignant-looking calcifications within the area of predominant benign calcifications yielded atypical ductal hyperplasia. Mammographic pepper, granular, punctate, salty and S-shaped calcifications were correlated with granular and salty calcification on light microscope. Mammographic linear, branching, comma, tadpole and wormiform calcifications were correlated with histopathologic laminated calcification. CONCLUSION: Authors have recognized about characteristics of malignant Vs. benign clustered microcalcifications on histopathology. Authors concluded that if malignant-looking calcification such as linear, branching, comma, tadpole and wormiform are present in an are a of predominant benign calcifications, specimen radiographic correlation and mapping study will be necessary for better demonstration of the atypical ductal hyperplasia, hidden or associated breast cancer.
Breast Neoplasms ; Humans ; Hyperplasia ; Larva ; Mammography* ; Paraffin

Maintenance of normal rate of tear production and elimination is essential for the proper controlled envionment for the cornea and the conjunctiva. We analyzed tear turnover rates in normal subjects, which mainly depend on tear production. We scanned 65 eyes of 36 normal subjects after instillation of 2% fluorescein solution 1u into the lower conjunctival fornix, using fluorophotometer (Fluorotron Master). After instillation of fluorescein solution, we scanned the eyes every 3 minutes for 30 minutes to measure tear film fluorescence. We calculated the tear turnover rate using tear film fluorescence valuse. Mean tear turnover rates were 12.3+/-6.6 %/min in normal subjects, 12.7+/-6.9 %/min in men, and 11.7+/-6.6 %/min in women. Mean tear turnover rates of men did not differ significantly from those of women (p>0.05). Mean tear turnover rates of younger subjects(25 years old~40 years old) (16.2 +/-7.4 %/min) were found to be significantly higher than ghose of older subjects(41 years old~73 years old) (9.1+/-40 %/min) (p<0.05). The morning tear turnover rates (13.5+/-1.6 %/min) were found to be higher than those for the afternoon (10.2+/-4.9 %/min), but the differences were not significant(p>0.05). We think that this study might be useful to study the diagnosis and treatement of tear secretory system disorders.
Conjunctiva ; Cornea ; Diagnosis ; Female ; Fluorescein ; Fluorescence ; Humans ; Male ; Tears*

Serum immunoglobulins from Israeli carp (I. carp) were purified using affinity chromatography. Fish were immunized with purified mouse IgG, and the specific fish antibodies purified from the immune serum on a mouse IgG-immobilized agarose gel. Rabbit anti-carp Ig (Raclg) antibodies were produced following hyperimmunization with mlgG specific I. Carp Ab. SDS-PAGE analysis under reducing condition showed that I. carp Ig (clg) were composed of two u-like heavy chains with about 82 and 50 kD, respectively and one light chain with about 25 kD. On immunoblotting analysis, however, Raclg failed to react with light chain. When both protein A and protein G purified normal clg were compared with mlgG specific clg, no significant structural differences among them were observed. To investigate if there is any homology between other fish Ig molecules, cross-reactivity of Raclg against Ig molecules from 6 different fish sera and mouse control serum was checked on immunoblotting analysis. As a result, Raclg responded to only carp and tilapia Ig molecules, indicating that both tilapia and carp are very closely associated, especially, in the genetic basis of immunoglobulin structure. In flow cytometry study, Raclg appeared to recognize 45.8% of carp Ig+, 14.5% of catfish Ig+ and <5% of tilapia Ig+ cells. The result suggest the heterogeniety between receptor immunoglobulins on B-like lymphocytes and soluble immunoglobulins in serum. It is crucial to obtain pure fish immunoglobulins to produce reagent antibodies as tools for the study of their specific immune response.
Animals ; Antibodies ; Carps* ; Catfishes ; Chromatography, Affinity ; Electrophoresis, Polyacrylamide Gel ; Flow Cytometry ; Immunoblotting ; Immunoglobulin G ; Immunoglobulins* ; Lymphocytes ; Mice ; Sepharose ; Staphylococcal Protein A ; Tilapia