1.Effect of functional endoscopic sinus surgery on olfactory disturbance caused by bilateral chronic sinusitis with nasal polyposis.
Chul Min PARK ; Sung Tae HONG ; Wan Suk JUN ; Jong Wook YUN ; Jae Seon LIM
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(4):693-697
No abstract available.
Sinusitis*
2.Testicular Volume of Korean Young Adult Men as Measured by Ultrasonography: Relationship with Body Mass Index.
Jong Wan LIM ; Jong Yoon BAHK ; Seung Ki MIN
Korean Journal of Urology 2009;50(6):591-595
PURPOSE: Testicular volume is one of the important indices that represent the reproductive ability of adult males. In previous studies, however, testicular volume was measured by manual palpation or orchidometer, resulting in inaccuracy or subjectivity. Accordingly, the need for accurate data on the size of the testes is increasing, and we conducted testicular volume measurements by using scrotal ultrasonography. We investigated the relationship between testicular volume and body weight, height, and body mass index (BMI). MATERIALS AND METHODS: The testicular volumes of 1,002 young adult Korean men were measured for length, width, and thickness with ultrasonography, and data on BMI were collected conventionally. The correlation between testicular volume and BMI was analyzed with one-way ANOVA and Student's t-test. RESULTS: The mean age of the young men was 23.02+/-2.53 years. The mean testicular volume was 18.09+/-3.79 cc on the left and 18.26+/-3.21 cc on the right. Testicular volume was directly proportional to body weight, height, and body mass index. CONCLUSIONS: The testicular volume of Korean young adult men was about 18.17 cc. Testicular volume has a weak direct correlation with body weight, height, and body mass index.
Adult
;
Body Mass Index
;
Body Weight
;
Humans
;
Male
;
Palpation
;
Testis
;
Young Adult
3.Intracranial hemorrhage in the prematurity and small for gestational age an attempt of clinico-ultrasonographic correlation.
Kwang Sup KIM ; You Seop SHIN ; Jong Wan KIM ; Kwang Nam KIM ; Ki Yang RYOO ; Hyo Keun LIM
Journal of the Korean Pediatric Society 1991;34(1):49-56
No abstract available.
Gestational Age*
;
Intracranial Hemorrhages*
4.Determination of trace elements in serum using inductively coupled plasma atomic emission spectrometry.
Jong Wan KIM ; Jon Kee LEE ; Chan Bin IM ; Gye Chul KWON ; Sun Hoe KOO ; Jong Woo PARK ; Myung Sub GAN ; Heung Bin LIM
Korean Journal of Clinical Pathology 1993;13(2):173-180
No abstract available.
Plasma*
;
Spectrum Analysis*
;
Trace Elements*
5.Evaluation of the Usefulness of Kobias HBsAg and Anti-HBs.
Joowon PARK ; Insoo RHEEM ; Do Young YOON ; Jong Seok LIM ; Jong Wan KIM
The Korean Journal of Laboratory Medicine 2003;23(6):411-414
BACKGROUND: The analysis of serological markers for hepatitis B virus (HBV) is a useful tool for the prevention and diagnosis of HBV infection. In this work, we evaluated a newly improved domestic rapid assay, Kobias HBsAg and anti-HBs Window kits (Kobias, Korea) for the detection of HBsAg and anti-HBs in serum. METHODS: A total of 360 sera screened by enzyme immunoassay (EIA) (Enzygnost, DADE Behring, Germany) were included in this study. Each specimen was tested for HBsAg and anti-HBs by Kobias Window kits and Genedia Rapid device (Green Cross, Korea), conventional one step test kits. The results were compared with those of EIA. RESULTS: The sensitivity and specificity of Kobias HBsAg were 99.2% and 96.7%, and those of Kobias Anti-HBs were 95.8% and 96.7%, respectively. The concordance rates between EIA and Kobias HBsAg and Kobias Anti-HBs were 98.3% and 96.1%, and those between Kobias kits and Genedia kits for HBsAg and anti-HBs were 97.8% and 93.9%, respectively. CONCLUSIONS: Kobias HBsAg/Anti-HBs kits are simple, rapid, and low-cost methods for detecting HBsAg and anti-HBs. With comparable results with EIA, the Kobias HBsAg/Anti-HBs kits could be suitable for screening purposes or in emergency situations.
Diagnosis
;
Emergencies
;
Exercise Test
;
Hepatitis B Surface Antigens*
;
Hepatitis B virus
;
Immunoenzyme Techniques
;
Mass Screening
;
Sensitivity and Specificity
6.Role of STAT3 as a Negative Regulator in Mac2- Binding Protein Expression.
Yuk Pheel PARK ; Jong Tae KIM ; Young YANG ; Jong Seok LIM ; Do Young YOON ; Jong Wan KIM ; Hee Gu LEE
The Korean Journal of Laboratory Medicine 2008;28(3):230-238
BACKGROUND: Mac-2 binding protein (Mac-2BP) is a secreted glycoprotein from the culture fluid of several human cancer cells, especially breast, lung, and gastric cells. Mac-2BP plays a role in immune response and cell adhesion activity in patients with various cancer and infectious diseases. In this study, we attempted to identify the regulators of Mac-2BP expression at the transcriptional level. METHODS: To determine the effect of epidermal growth factor (EGF) to Mac-2BP expression in gastric cancers, we constructed the different lengths of Mac-2BP promoter plasmids and measured the promoter activity and Mac-2BP expression. In addition to investigating the role of signal transducer and activator of transcription3 (STAT3) or human telomerase reverse transcriptase (hTERT) as a regulator of Mac-2BP, we transfected the small interfering RNA (siRNA) specific for STAT3 or hTERT, and Mac-2BP level was observed by a quantitative ELISA. RESULTS: EGF treatment could suppress the Mac-2BP transcription in HEK293 or gastric cancer cell lines (SNU-638 or AGS). In 5'-deleted promoter experiment, pGL3-Mac Pro-2377 transfected cells showed a decreased luciferase activity compared to pGL3-Mac Pro-2277. We also identified that (-2,366/-2,356) on Mac-2BP promoter is a putative STAT3 binding site and suppression of STAT3 with STAT3 specific siRNA increased the Mac-2BP level, suggesting the role of STAT3 as a negative regulator, in contrast to hTERT, which is known as a positive regulator. CONCLUSIONS: EGF signal is critical for the Mac-2BP expression, and more importantly, STAT3 could work as a negative regulator, while hTERT as a positive regulator in Mac-2BP transcription.
Antigens, Neoplasm/genetics/*metabolism
;
Cell Line
;
Cell Line, Tumor
;
Down-Regulation
;
Epidermal Growth Factor/metabolism
;
Humans
;
Membrane Glycoproteins/genetics/*metabolism
;
RNA, Small Interfering
;
STAT3 Transcription Factor/genetics/*metabolism
;
Telomerase/metabolism
;
Transfection
7.A Case of Familial Adenomatous Polyposis (FAP) with A Large Sentinel Polyp.
Dae Ghon KIM ; Byung Hyun RHEE ; Seong Hee LIM ; Wan Hee YOO ; Jae Yong KWAK ; Deuk Su AHN ; Jong Hun KIM ; Dong Geun LEE
Korean Journal of Gastrointestinal Endoscopy 1995;15(4):775-782
Familial adenomatous polyposis(FAP) is a rare hereditary disorder characterized by the development of hundreds to thounds polyps throughout the colon and rectum. Moreover, several extracolonic manifestations are seen. Recently, this disease is recognized as a adenomatous polyposis syndrome which can involve the entire astrointestinal tract. Several reports have demonstrated a high incidence of gastroduodenal polyps in patients with familial adenomatous polyposis. These colon polyps can be eventually developed as colon cancer, if not be treated. So early diagnosis is needed and prophylactic surgery should be erformed. We experienced a case of familial adenomatous polyposis with a large sentinel polyp on the sigmoid colon, presenting hematochezia and mucoid diarrhea. He was early treated before progression to carcinoma by total colectomy, rectal mucosectomy and J pouch ileoanal anastomosis.
Adenomatous Polyposis Coli*
;
Colectomy
;
Colon
;
Colon, Sigmoid
;
Colonic Neoplasms
;
Colonic Pouches
;
Diarrhea
;
Early Diagnosis
;
Gastrointestinal Hemorrhage
;
Humans
;
Incidence
;
Polyps*
;
Rectum
8.Transcriptional Regulation of H2B Histone Gene Expression by Nocodazole in HL-60 Cells.
Kyu LIM ; Ye Gi HONG ; Myung Sun LEE ; Mee Young SON ; Kyung Ah YUN ; Jong Il PARK ; Wan Hee YOON ; Sung Kiel PARK ; Byung Doo HWANG
Journal of the Korean Cancer Association 2000;32(2):407-416
PURPOSE: Nocodazole, a microtubule disrupting reagent, is known to arrest cells in the M phase, To gain insight on the regulatory mechanism of H2B histone gene expression by nocodazole in HL-60 cell, the binding pattern of nuclear proteins to cis element in the human H2B histone gene promoter has been investigated with DNase I footprinting and DNA mobility shift assay. MATERIALS AND METHODS: Northern blot hybridization was performed by the method of Virca et al. A Hinc II-Sac I fragment of pSPH28 was used as probe for Northern blot analysis of H2B histone mRNA. DNase I footprinting and DNA mobility shift assay were performed by the method of Lim et al. End labeled DNA oligomer (upper strand, 5'-CTTCACCTTATTTGCATAA GCGATTC-3') for octamer binding activity was mixed with nuclear extracts in a 20 ul reaction volume containing 60 mM KC1, 12 mM HEPES, pH 7.9, 5 mM MgCl2, 0.2 mM EDTA, 0.2 mM DTT, 12% glycerol, and 2 ug of poly [dI-dC]. RESULTS: The level of H2B histone mRNA rapidly was reduced at 24 hours in nocodazole-treated HL-60 cells and the mRNA was repressed in proportion to the concentration of nocodazole. Nocodazole-dependent repression of H2B histone gene was restored by replacement with nocodazole-free media. In DNase I footprinting analysis, one nuclear factor bound at 42 bp site (octamer motif) in the absence of nocodazole. In the presence of nocodazole, the binding of nuclear factor on octamer motif partially vanished. In DNA mobility shift assay, one DNA-protein complex (Octl) was formed when octamer motif was incubated with nuclear extract of HL-60 cell. After nocodazole treatment, Octl binding activity was reduced by time dependent manner. CONCLUSION: These results suggest that nocodazole-dependent repression of H2B histone gene is correlated with reduction of Octl binding activity in HL-60 cell.
Blotting, Northern
;
Cell Division
;
Deoxyribonuclease I
;
DNA
;
Edetic Acid
;
Electrophoretic Mobility Shift Assay
;
Gene Expression*
;
Glycerol
;
HEPES
;
Histones*
;
HL-60 Cells*
;
Humans
;
Hydrogen-Ion Concentration
;
Magnesium Chloride
;
Microtubules
;
Nocodazole*
;
Nuclear Proteins
;
Repression, Psychology
;
RNA, Messenger
9.Role of ATF on Transcriptional Regulation of DNA Topoisomerase II a Gene in HL - 60 Arrested to G2 / M and M Phase.
Kyu LIM ; Mee Young SON ; Byung Ik CHOI ; Kyung Ah YUN ; Meizi ZHENG ; Tae Wook KANG ; Young Chul LEE ; Jong II PARK ; Wan Hee YOON ; Byung Doo HWANG
Journal of the Korean Cancer Association 1999;31(6):1279-1287
PURPOSE: To gain insight on transcriptional repression of Topo II a in HL-60 cells arrested to G2/M and M phase, the levels of Topo IIa mRNA and the binding activity of ATF have been investigated with Northern blot hybridization and DNA mobility shift assay, respectively. MATERIALS AND METHODS: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-mactivated fetal bovine serum and antibiotics in a humidified 5% CO2 at 37C degree. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. A Xho I-Mlu I fragment of phTOP2 was used as probe for Northern blot analysis of Topo II a mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA oligomer (upper strand, 5-TCTCCGCTATGACGCCGAGTGGTG-3) for ATF binding activity was mixed with nuclear extracts in a 20 pl reaction volume containing 60 mM KC1, 12 mM HEPES, pH 7.9, 5 mM MgCl2, 0.2 mM EDTA, 0.2 mM DTT, 12% glycerol, and 2 ug of poly [dI-dC]. RESULTS: HL-60 cells were arrested at G2/M phase and M phase after taxol or nocodazole treatment. The levels of Topo II a mRNA were reduced at 24 hours after exposure with nocodazole or taxol but the unknotting activities were not changed. DNA mobility shift assay using oligonucleotide containing the ATF binding site showed that ATF binding activity was reduced after pretreatment of nododazole or taxol. CONCLUSIONS: These results suggest that the reduction of ATF binding activity may be important to transcriptional repression of Topo II a gene by nocodazole and taxol in HL- 60 cells.
Anti-Bacterial Agents
;
Binding Sites
;
Blotting, Northern
;
Cell Division*
;
DNA Topoisomerases, Type I*
;
DNA Topoisomerases, Type II*
;
DNA*
;
Edetic Acid
;
Electrophoretic Mobility Shift Assay
;
Genes, vif
;
Glycerol
;
HEPES
;
HL-60 Cells
;
Humans
;
Hydrogen-Ion Concentration
;
Magnesium Chloride
;
Nocodazole
;
Paclitaxel
;
Repression, Psychology
;
RNA
;
RNA, Messenger
10.Acquired Huge Calyceal Diverticulum After Renal Injury.
Moung Jin LEE ; Jae Young CHOI ; Seung Hun CHO ; Jong Wan LIM ; Seung Tae LEE ; Seung Ki MIN
Journal of the Korean Society of Traumatology 2009;22(2):264-268
The calyceal diverticulum is a cystic cavity lined by a transitional epithelium, is encased within the renal substance, and is situated peripheral to a minor calyx, to which it is connected by a narrow channel. Both congenital and acquired factors have been suggested to explain the formation of a calyceal diverticulum. We experienced a case of a huge calyceal diverticulum that was newly developed after a renal injury.
Diverticulum
;
Epithelium
;
Kidney