Recently, we found that the Korean traditional medicine could enhance the expression of some cytokine in murine bone marrow cell culture. To understand the mode of actions of the traditional medicine, we examined the pattern of protein phosphorylation in bone marrow cells treated with Korean traditional medicine. When bone marrow cells were treated with Korean traditional medicine, the phosphorylation of p45, p50, p84/p91 kinase was observed, and gene expression of TPO and SCF was related to p45 and p91. It had been reported that TPO, SCF, IL-3 and GM-CSF gene expression transmits signal through the JAK-STAT pathway. Molecular involvement of JAK-STAT in signal induced by Korean traditional medicine was analyzed by anti-JAK and anti-STAT-1 Ab. To analyze the transcriptional factor that promotes the cytokine gene expression by the stirnulation of Korean traditional medicine, GAS and NF-B binding activity was analyzed by EMSA. We found the increase in NF-B at SMT and BHT. Therefore, we conclude that the cytokine gene expression induced by Korean traditional medicine was mediated by the JAK-STAT pathway and by the combination of GAS and NF-kB.
Bone Marrow Cells ; Butylated Hydroxytoluene ; Gene Expression ; Granulocyte-Macrophage Colony-Stimulating Factor ; Hematopoiesis* ; Interleukin-3 ; Medicine, Korean Traditional* ; Medicine, Traditional ; NF-kappa B ; Phosphorylation ; Phosphotransferases ; Signal Transduction

The Korean traditional medicinal (KTM) herbs such as Angelicae gigantis, Rehmannia glutinosa, Paeoniae lactiflora, Cnidii officinale, Salviae miltiorrhizae, and Millettia reticulata, and prescribed formula Samultang, and Bohyultang improve the function of the hematopoietic system and nourishing the body to promote health. However, there are limited scientific background on the mode of action of these medicine. To understand the diverse actions following traditional medicine stimulation, we determined the production of hematopoietic cytokine, thrombopoietin, stem cell factor, and interleukin-3 (IL-3) in KlM treated bone marrow cells. When bone marrow cells were treated with KTM, the expression of hematopoietic cytokine were increased in RT-PCR and ELISA analysis. Furthermore, when the bone marrow cells were separated into adherent celis and suspension cells and were treated with KTM, we found the increase of TPO gene expressions in suspension cells. Meanwhile, other hematopoietic cytokine gene expression in bone marrow cells was higher as whole than when adherent cells and suspension cells was separated. Therefore, we could know the interaction of two cells that increases the cytokine expression. These results suggest that KTM has hematopoietic effects through increasing the production of cytokine.
Angelica ; Bone Marrow Cells ; Enzyme-Linked Immunosorbent Assay ; Gene Expression ; Hematopoiesis* ; Hematopoietic System ; Interleukin-3 ; Medicine, Korean Traditional* ; Medicine, Traditional ; Millettia ; Paeonia ; Rehmannia ; Salvia ; Stem Cell Factor ; Thrombopoietin

No abstract available.
Androgen-Insensitivity Syndrome* ; Male

We report a case of disseminated calcific cysticercosis of brain, accompanied with marked hydrocephalus. Histopathologically, the calcific spots consisted of mummified scolex and bladder of cysticercus cellulosae with a diffuse calcification, but no area of cystic change were noted. Surrounding brain tissue showed a dense fibrous reaction and gliosis with little foreign body reaction.
Brain* ; Cysticercosis ; Cysticercus ; Foreign-Body Reaction ; Gliosis ; Hydrocephalus ; Urinary Bladder

No abstract available.
Meningitis, Aseptic*

No abstract available.
Humans ; Infant, Newborn* ; Osmolar Concentration* ; Specific Gravity*

No abstract available.
Bilirubin* ; Humans ; Infant, Newborn*

BACKGROUND: Dopamine receptors have been regarded as a strong candidate involved in etiology of schizophrenia and a target for various antipsychotic drugs. The purpose of our study was to investigate whether dopamine D1 receptor(DRD1) gene polymorphisms would predict the treatment response to antipsychotics in schizophrenia. METHOD: One hundred thirty-four schizophrenic patients, who met DSM-IV criteria for schizophrenia were entered into a 48-week study. The psychopathology of the patients was assessed at baseline, 12th, 24th, 48th weeks of treatment by PANSS. Responders were defined by a 20% of the reduction in total PAnSS score at end point. The genomic DNA fragment corresponding to nucleotides of dopamine D1 receptor gene was amplified by polymerase chain reaction(PCR). RESULT: Neither allelic frequencies nor genotypes for dopamine D1 receptor differed significantly between responders and non-responders. Also, there was no difference of changes of PANSS scores among three genotype groups of the dopamine D1 receptor. CONCLUSION: Allelic variation in the dopamine D1 gene is not associated with individual differences in antipsychotic response.
Antipsychotic Agents ; Diagnostic and Statistical Manual of Mental Disorders ; DNA ; Dopamine* ; Genotype* ; Humans ; Individuality ; Nucleotides ; Psychopathology ; Receptors, Dopamine ; Receptors, Dopamine D1* ; Schizophrenia

BACKGROUND: The angiotensin-converting enzyme inhibitors have been found to be safe and efficacious in the treatment of essential hypertension. Fosinopril is the first angiotensin-converting enzyme inhibitor from a new class of agents containing phosphorus. This drug is known to be metabolized to almost and equal extent by the hepatic and renal pathways. METHODS: This study was performed to investigate the efficacy and safety of oral fosinopril, a new class of phosporus-containing angiotensin converting enzyme inhibitor, on essential hypertension. A single daily dose of 10mg to 20mg fosinopril was administered in 21 hypertensive patients with diastolic blood pressure in the range of 95mmHg-115mmHg while off all other anti-hypertensive agents for 10 weeks. Blood pressure and heart rate were measured every 4 weeks. The complete blood count, blood chemistry by SMA-12, serum electrolytes and urinalysis were performed at 12th week of therapy. RESULTS: 1) Baseline systolic and diastolic blood pressures after 2 weeks of placebo at sitting position were 158.8+/-15.7 and 99.4+/-6.3mmHg respectively. There was a statistcally significant reduction of blood pressure after 4 week treatment of fosinopril which was maintained up to 12 weeks of follow-up(158.8+/-15.7-99.4+/-6.3mmHg vs 139.3+/-18.2/86.6+/-10.3mmHg, p<0.05). 2) The proportion of responders defined by diastolic blood pressures less than 90mmHg or decline more than 10mmHg at 4, 8 and 12 weeks after treatment with fosinopril were 90.5, 95.2, and 95.2% respectively. 3) THere were no significant changes in blood chemistry, serum electrolytes, hematologic findings and heart rate over the treatment period. 4) Three patients experienced severe non-productive cough that required to discontinue the medication. CONCLUSION: In patients with mild to moderate hypertension, once-daily fosinopril(10mg and 20mg) provided significant anti-hypertensive effects without serious side effects. The 10mg dose was effective in majority of patients and may be considered as a starting dose.
Angiotensin-Converting Enzyme Inhibitors ; Antihypertensive Agents ; Blood Cell Count ; Blood Pressure ; Chemistry ; Cough ; Electrolytes ; Fosinopril* ; Heart Rate ; Humans ; Hypertension ; Peptidyl-Dipeptidase A ; Phosphorus ; Urinalysis

Pulmonary function test is one of the most important tools in the management of patients with respiratory tract diseases and allergic diseases. As with the difficulties in performing the test, it has been seldom used in pediatric field. But with the advent of simple, computerized tools, pediatric pulmonologist and allergist try to manage the patients using PFT. Still the normal predicted values are variable among the reports. So it is important to have normal predicted value in Korean children. From April to July 1992, pulmonary function test was performed in 965(male: 490, female: 475) primary school-aged children except <3 or >97 percentile of Korean-children Physical developemental standards with history of allergic diseases and respiratory tract diseases ay SungNam, KyongKiDo. We evaluated the predicted normal values of the FEV1.0, FVC and PEFR and logarithmic regression equation setting the predicted values by using the microspirometer of Micromedical Ltd. England that could that could be easily applied to children. 1) Predicted values of pulmonary function test items were generally higher in boys than those of girls. 2) Correlation coefficient to the parameters examined was the highest in height 0.78, then age 0.75, weight 0.70 and chest circumference 0.61.
Child* ; England ; Female ; Gyeonggi-do ; Humans ; Peak Expiratory Flow Rate ; Reference Values ; Respiratory Function Tests* ; Respiratory Tract Diseases ; Thorax