1.Clinical Aspects of Hydrops Fetalis.
Korean Journal of Obstetrics and Gynecology 2000;43(8):1326-1330
No abstract available.
Edema*
;
Hydrops Fetalis*
2.A Study on the Tumor Angiogenesis and Expression of Cytokine and Growth Factors in the Prostatic Carcinoma.
Korean Journal of Pathology 1996;30(8):671-679
There is considerable experimental evidence to indicate that tumor growth is dependent on angiogenesis. However, we do not understand how the angiogenic activity is initiated by a given tumor. There is a clear distinction between a stage without neovascularization, which correlates with a paucity of metastases, and a stage in which increasing neovascularization correlates with a rising rate of metastasis. The authors therefore asked whether the extent of angiogenesis in human prostatic carcinoma is correlated with the tumor grades or some growth factors. To investigate how tumor angiogenesis correlates with tumor aggressiveness, the authors counted microvessels within the various grades of invasive prostatic carcinomas of 44 patients and the nodular hyperplasias of 10 patients. Highlighting of the vessels by immunohistochemical staining for factor VIII-related antigen and assessment of the tumor aggressiveness by the degree of expression of some growth factors(transforming growth factor-alpha, and beta, epidermal growth factor), tumor necrosis factor-alpha and tumor grading(Gleason's score) were done. As a result, both microvessel counts and the expression of growth factors and tumor necrosis factor correlated with tumor grades. In conclusion, the number of microvessels per 200 X fields in the areas of most intense neovascularization in a prostatic carcinoma may be a predictor of the patient's prognosis. Therefore, assessment of tumor angiogenesis may prove valuable in selecting patients with prostatic carcinoma, especially small needle biopsy, for aggressive therapy.
Humans
;
Tumor Necrosis Factor-alpha
3.Repair of Defect Caused by Surgical Removal of Skin Cancers by Secondary Intention.
Seok Jong LEE ; Young Min JEON
Korean Journal of Dermatology 1999;37(3):325-331
BACKGROUND: There are a few methods, including primary intention, deleyed primary closure and secondary intention, to irpair several types of defects. But commonly-used primary intentional repair of a defect after surgical removal of a skin cancer by graft, flap or simple closure has several limitations such as the need of a skillful technique and various complications. OBJECTIVE: We undertook a secondary intention using occlusive dressing with a polyurethane film after skin cancer surgery instead of a primary repair and evaluated its efficacy, particularly in points of simplicity and safety, and cosmetic results. METHODS: We randomly chose 14 cases of skin cancers in 13 patients who had undergone simple surgical excision or Mohs micrographic surgery and then undertook secondary intention with polyurethane film after informed consent. Postsurgical wound care included cleansing with normal saline or boric acid, then covering the defect with antibiotic ointment, gauze and film a at regular intervals.
Humans
;
Informed Consent
;
Intention*
;
Mohs Surgery
;
Occlusive Dressings
;
Polyurethanes
;
Skin Neoplasms*
;
Skin*
;
Transplants
;
Wounds and Injuries
4.Establishment and Characterization of a Small Cell Lung Cancer Cell Line(JePa-1).
Mi Ja LEE ; Ho Jong JEON ; Jong Hoon CHUNG
Korean Journal of Pathology 1997;31(8):695-710
Lung cancer is the most common malignant tumor worldwide and its incidence continues to rise each year. Recent development of molecular biologic method has led to advances in determining the etiologic factors of lung cancer and the establishment of cell lines has provided a lot of information on the through chemosensitivity, radiation biology studies, cytogenetics, and molecular biologic studies, which permits improved treatment for lung cancer. We established a small cell lung cancer cell line, designated JePa-1, obtained from malignant pericardial effusion of small cell lung cancer patient and characterized its morphologic and molecular biologic features. the JePa-1 cell line grew relatively slowly (doubling time 45hrs) as very loosely adherent floating aggregates growing in small clumps with distinct cell outlines and intertwined cords. Also JePa-1 cell line secreted antidiuretic hormones. Electronmicroscopic examination revealed that JePa-1 cell line and xenografts contained electron dense core granules, characteristic of being of neuroendocrine origin. To investigate the tumorigenic capacity, the JePa-1 cell line was injected into SCID and nude mice. Tumors taken from xenografts were observed in 3 out of 4 of the SCID mice and 2 out of 4 of the nude mice. The histologic characteristics of the xenografts were similar to those of the cell line and the original cytologic finding of the pericardial fluid, suggesting small cell carcinoma. The results of immunohistochemical markers showed reactivity for Rb protein, c-myc, TGF-alpha, TGF-beta , EGFR, keratin, NSE, chromogranin, and EMA. The DNA ploidy and the index of the JePa-1 cells was tetraploid and 2.13, respectively. The positive rate for the Rb, c-myc and K-ras proteins of the JePa-1 cell line were 98.9%, 99.3%, and 99.7% respectively as determined by flow cytometry. Cytogenetic analysis using the G-banding technique showed 65 chromosomes with various numerical and structural abnormalities. On examination of the expression of TGF-alpha, TGF-beta , and EGFR by PCR, only the EGFR was positive Through the establishment of JePa-1 cell line, we report in this paper the characterization of a small cell lung cancer such as morphologic and immunocytochemical features, growth characteristics in culture, hormone production, expression of oncoprotein and several growth factors, tumorigenicity, chromosomal abnormalities, and DNA ploidy and index. The JePa-1 cell line will be valuable in vitro studies for the etiology, treatment and the prognostic factors in small cell lung cancer.
Animals
;
Carcinoma, Small Cell
;
Cell Line
;
Chromosome Aberrations
;
Cytogenetic Analysis
;
Cytogenetics
;
DNA
;
Flow Cytometry
;
Heterografts
;
Humans
;
Immunohistochemistry
;
Incidence
;
Intercellular Signaling Peptides and Proteins
;
Lung Neoplasms
;
Mice
;
Mice, Nude
;
Mice, SCID
;
Pericardial Effusion
;
Ploidies
;
Polymerase Chain Reaction
;
Radiobiology
;
Retinoblastoma Protein
;
Small Cell Lung Carcinoma*
;
Tetraploidy
;
Transforming Growth Factor alpha
;
Transforming Growth Factor beta
;
Vasopressins
5.Significance of Expression of p16, Cyclin D1, Rb, and p53 Protein and Correlation with Clinicopathologic Prognostic Factors in Invasive Ductal Carcinoma of the Breast.
Mi Ja LEE ; Ho Jong JEON ; Kweon Cheon KIM
Korean Journal of Pathology 2000;34(4):288-299
The retinoblastoma (Rb)/cyclin D1/p16 pathway is an important constituent of cell cycle regulation. Perturbations in this pathway due to a variety of genetic aberrations have been reported in many human cancers including breast cancer. We examined the significance of immunoexpression of p16 protein, cyclin D1 protein, Rb protein (pRb), and p53 protein in 128 cases of invasive breast carcinoma. The results were correlated with survival rate and clinicopathological variables, including age, histologic grade, lymph node status, tumor size, estrogen receptor (ER), and progesterone receptor (PR) content. Abnormal expressions of p16 and pRb which were defined as negative staining were seen in 21% and 43% of tumors, respectively. There was a significant inverse relationship between p16 and pRb expression. There was no correlation between p16 staining and any other parameters, including survival rate, cyclin D1, p53, and clinicopathologic variables. Surprisingly, there was a trend for tumors which were positive for pRb to be grade III ductal carcinomas. Cyclin D1 positivity was noted in 46% of cases. The expression of cyclin D1 protein was significantly higher in lower histologic grade, higher ER and PR expression. The expression of p53 protein showed a significant correlation with high tumor grade. In a Cox multivariate analysis, neither p16, pRb, cyclin D1 nor p53 was an independent predictor, but tumor size and lymph node status were independent predictors of patient outcome.
Breast Neoplasms
;
Breast*
;
Carcinoma, Ductal*
;
Cell Cycle
;
Cyclin D1*
;
Cyclins*
;
Estrogens
;
Humans
;
Lymph Nodes
;
Multivariate Analysis
;
Negative Staining
;
Receptors, Progesterone
;
Retinoblastoma
;
Retinoblastoma Protein
;
Survival Rate
6.Significance of Expression of bcl-2, p53 and cyclin D1 and Their Correlation with Clinicopathologic Prognostic Factors and Survival Rate in 128 Cases of Invasive Breast Carcinoma.
Suk Hyun PARK ; Mi Ja LEE ; Ho Jong JEON
Korean Journal of Obstetrics and Gynecology 2000;43(8):1360-1367
No abstract available.
Breast Neoplasms*
;
Breast*
;
Cyclin D1*
;
Cyclins*
;
Survival Rate*
7.Ultrastructural In Situ Hybridization Method for Detection of Oncogenic Viruses.
Keun Hong KEE ; Chul Hee CHOI ; Ho Jong JEON
Korean Journal of Pathology 1996;30(8):687-698
In situ hybridization (ISH) is a standard method for localizing DNA or RNA sequences in tissue or cell preperation. The technique was developed at the electron-microscopic level, and enables the precise subcellular localization. A method was developed for detection of specific viral DNA. We have tested various methods and technique to detect specific viral DNA through ISH at the electron microscopic level. Postembedding method of ultrastructural ISH was developed and successfully applied for the detection of human papillomavirus type 16 in squamous cell carcinoma of the uterine cervix and Epstein-Barr virus in EBV-infected leukemia cell line. The following results are made. The best results were obtained using 0.2% glutaraldehyde and 4% paraformaldehyde fixed tissue or cell block. The labelling was best observed on Unicryl resin and Lowicryl K4M resin sections. Epon sections showed no reactivity. Thin sections of Unicryl resin were more easier than Lowicryl K4M resin. Enzymatic predigestion with proteinase K, pepsin and trypsind gave good results. However, high concentration of these produce poor results due to excessive destruction of the cellular components. Alkali treatment with 0.5N sodium hydroxide produced successful results in denaturation of target DNA. The labelling density of gold particles was independent of incubation time or temperature in hybridization step. The viral DNA labelling was localized mainly within the nucleus, both within and at the edge of electron dense regions, and below the nuclear membrane. And the labelling was seen in the form of a dense, roughly spherical shape. In conclusion, the best results are obtained by the conditions that tissue fixed by 0.2% glutaraldehyde and 4% paraformaldehyde solution, embedded with Unicryl resin, protein denaturation by 0.1ul/ml proteinase K, DNA denaturation by 0.5N sodium hydroxide, and reaction with DNA probe.
Humans
8.A Case of Livebirth by Delayed Interval Delivery in Twin Pregnancy.
Yong Joon JEON ; Jong In KIM ; Jeong Ho RHEE
Korean Journal of Perinatology 2001;12(2):168-172
No abstract available.
Humans
;
Pregnancy, Twin*
9.A case of hemolytic disease of newborn due to anti-E.
Sook Ja PARK ; Eun Ju JONG ; Seong Sook JEON
Korean Journal of Blood Transfusion 1993;4(1):117-121
No abstract available.
Erythroblastosis, Fetal*
;
Infant, Newborn
10.Immunohistochemical studies on neuroendocrine cell changes in disease of uterine cervix.
Sei Jun HAN ; Kyung Sig CHANG ; Ho Jong JEON
Korean Journal of Obstetrics and Gynecology 1992;35(8):1144-1153
No abstract available.
Cervix Uteri*
;
Female
;
Neuroendocrine Cells*