Root conditioning has been introduced to dissolve the smear layer, and to aid in detoxification following root planing. It produces surface demineralization, resulting to exposure of dentin collagen fibrils and opening of dentinal tubules. The purpose of the present study was to examine the possibility of obtaining root conditioning effect by using ethylenediamine tetraacetic acid(EDTA) solution instead of low pH etchants such as citric acid and tetracycline hydrochloride(Tc-HCl). Total 48 root specimens(4x5x2mm) were prepared from the mesial and distal root of 24 periodontitisaffected human mandibular molars after scaling and root planing. The specimens were treated with EDTA(17%, neutral pH, Pulpdent?, Pulpdent Co., USA) for 1 min., 2 min., and 3 min., and tetracycline hydrochloride(100mg/ml) for 5 min. using the rubbing technique and the placement technique without rubbing. The dentinal tubules of the specimens were examined under a scanning electron microscopy(JEOL, JSM- 840A, Japan). On the microphotographs taken at x3,000 magnification, the number of identifiable dentinal tubules, opened dentinal tubules, and fully opened dentinal tubules(over 0.9micrometer in diameter) per the unit area(1,067?m2) were counted. And the rate of opened dentinal tubule and the rate of fully opened dentinal tubule were calculated. The difference of those rates among all groups was statistically analyzed by ANOVA and Dunkan test. The results were as follows; 1. In the specimens applied with EDTA, the rate of opened dentinal tubule was not significantly different between each group regardless of application technique and application time(p>0.1). 2. In the specimens applied with EDTA, the rate of fully opened dentinal tubule had tendency to be increased in proportion to application time regardless of application technique. 3. In the specimens applied with EDTA within 2 min., the rate of fully opened dentinal tubule failed to show significant difference between the two techniques(p>0.1). But, in the specimens applied for 3 min., the rate of fully opened dentinal tubule was significantly higher in the specimens applied by the rubbing technique than those by the placement technique(p<0.001). 4. In the specimens applied with Tc-HCl for 5 min., both the rate of opened dentinal tubule(p<0.01) and the rate of fully opened dentinal tubule (p<0.001) were induced significantly higher by the rubbing technique than the placement technique. 5. The rate of fully opened dentinal tubule was significantly higher in both the specimens rubbed for 3 min. with EDTA and the specimens rubbed for 5 min. with Tc-HCl than other groups(p<0.001). But there was no significant difference between the two(p>0.1). The results demonstrate that 17% EDTA solution can replace low pH etchants for root conditioning, and the rubbing technique for at least 3 min. is recommendable.
Humans

Extracellular matrix component is degraded by enzymes of thematrix metalloproteinases(MMPs). MMPs are produced by both hemopoietic and structural cells. Increased activity of MMP-3 in periodontium is strongly associated with inflammatory periodontal disease. The purpose of the present study was to determine the effect of tetracycline analogues on the activity of MMP-3. Tetracycline-HCl, doxycycline-HCl, and minocycline-HCl were applied to huamn gingival fibroblasts at various concentrations of 10, 25, 50, 100, 200microgram/ml, and 1 hour later IL-1beta of 25ng/ml was added. After incubation for 24 hours the cells were reacted by enzyme-linked immunosorbent assay using proMMP-3 ELISA kit. The optical density was measured by microwell plate reader at 450nm. The relative activity of MMP-3 was calculated as the percentage of the optical density of each experimental group to that of the control. The difference of the optical density and the relative activity of MMP-3 between the experimental groups and the control wasstatistically analyzed by one way ANOVA. The results were as follows: 1. Tetracycline-HCl showed the tendency to inhibit the activity of MMP-3 at the concentration lower than 25 microgram/ml, but increased significantly the activity of MMP-3 at the concentration of 200microgram/ml(p<0.05). 2. Doxycycline-HCl inhibited significantly the activity of MMP-3 at the concentration lower than 100microgram/ml, but increased significantly the activity of MMP-3 at the concentration of 200microgram/ml(p<0.05). 3. Minocycline-HCl inhibited the activity of MMP-3 at the concentration in the range of 10 to 200microgram/ml. Within the limit of the present study, the above results suggested that the low concentration of tetracycline analogues could inhibit the activity of MMP-3 induced by IL-1beta in human gingival fibroblasts.
Enzyme-Linked Immunosorbent Assay ; Extracellular Matrix ; Fibroblasts* ; Humans ; Interleukin-1beta ; Matrix Metalloproteinases ; Periodontal Diseases ; Periodontium ; Tetracycline*

No abstract available.
Carcinoma, Non-Small-Cell Lung* ; Drug Therapy, Combination*

No abstract available.
Diagnosis* ; DNA* ; Polymerase Chain Reaction* ; Tuberculosis*

OBJECTIVES: Although there were many studies examining anxiety and depression in hospitalized medically-ill patients, there were few studies in examining anxiety and depression of non-psychiatric outpatients or comparative studies among the disease categories. Therefore, we wanted to explore 1) psychopathologies in the patients with chronic renal failure and those with chronic hepatitis, 2) differences of psychopathologies by severity, 3) differences of psychopathologies by the duration of illness, and 4) psychiatric consultation. This was the second series of the whole project. METHODS: From March to April 1999, 38 patients with chronic renal failure and 26 patients with chronic hepatitis who visited the medical outpatient department are included in the subject group and 116 persons who visited the health examination center in September 1999 are included in the control group. We reviewed the medical records of the patients and inquired what they thought the causes of their illness were. We evaluated the patients with State-trait anxiety inventory, Beck depression inventory, Symptom checklist-90-Revision. RESULTS: In state anxiety, 62.2% of the patients with chronic renal failure fell into the anxious group, which is somewhat greater than the 52.0% of those with chronic hepatitis. The rates in both subjects are significantly higher than that of the control group. 16.7% of the patients with chronic renal failure were depressed, which is somewhat greater than the 9.1% found in chronic hepatitis. The rates in both subjects showed no significant difference from that of the control group. When the state anxiety scores were compared during the duration of the illness, it was found to be relatively higher during the early phase of disease in both the chronic renal failure patients and the chronic hepatitis patients. In the depression, the scores were found to be relatively higher in the chronic renal failure patients when the duration of illness was between 1 to 5 years compared to other duration groups, while the patients of chronic hepatitis were found to be relatively constant. Most frequently, 36.2% of the patients with chronic renal failure and 24.3% of those with chronic hepatitis thought the cause of their illness to be 'psychological', but the rates of psychiatric consultation in both subjects are 5.7%, 7.7% respectively. CONCLUSIONS: Our results suggest that proper psychiatric interventions are not being conducted yet and more cooperative and integrative roles are required among psychiatrists and internists.
Anxiety ; Depression ; Hepatitis, Chronic* ; Humans ; Kidney Failure, Chronic* ; Medical Records ; Outpatients* ; Psychiatry

No abstract available.
Animals ; Muscle, Smooth, Vascular* ; Nitric Oxide* ; Rats*

No abstract available.
Lung Neoplasms* ; Lung* ; Proliferating Cell Nuclear Antigen*

No abstract available.
Lung Neoplasms* ; Lung*

PURPOSE: The aim of this study was to design and evaluate a scale for measuring reliability and validity in the field of oriental nursing education. METHOD: A questionnaire was administered to 495 university nursing students using a convenience sampling method. The period of time for collecting data was from September 2003 to January 2004. RESULT: The derived outcome tool consisted of 6 factors and 22 inquires on the basis of a conceptual frame of three domains (knowledge, attitude, and skill), As a result of the item analysis, 22 items were selected and the internal consistency alpha coefficient was .767. The value of Cronbach' Alpha of knowledge(factor 1) was .885, attitude(factor2)was .756, and skill (factor 3) was .610. The three factors accounted for 65.110% of the variance in the total scale. Addressing the explanatory variance of each domain Cognitive domain was 22.477%, affective domain was 20.543%, and psychomotor domain was 17.090%. CONCLUSION: Further studies need to be done to verify educational evaluation and apply our outcomes to oriental nursing education.
*Medicine, Oriental Traditional ; Korea ; Humans ; Female ; Educational Measurement ; *Education, Nursing ; Adult

Organ shortage is a serious problem in the field of solid organ transplantation. Increasing number of death on the waiting list, transplant tourism, black market for organ selling are all caused by organ shortage and these eventually causing poor quality of life for patient and family, and may give rise to a serious confusion in domestic transplant system. Since the KONOS launched in the year 2000, some portion of the illegal side of organ supply were corrected but the number of organ donor was hardly to increase. In order to search any solution for this problem, organ allocation study group under the Korean society for organ transplantation was actively worked from August 2008 through February 2009, and got some solution. Among them, amendment of the transplantation law including brain death committee, reporting system of suspected brain dead patients, and set up an independent organ procurement organization system for an effective organ procurement. Organ donation and increasing the number of donor is not a task only for transplant society, but is closely related with quality of life for peoples. This also can change the execution of budget of national medical health insurance. To give a correct understanding about this and activate the nationwide organ donation, the transplant society should have a key role with various medical and nursing society, hospital association, government, national assembly and every voluntary groups.
Brain Death ; Budgets ; Humans ; Insurance, Health ; Jurisprudence ; Organ Transplantation ; Quality of Life ; Societies, Nursing ; Tissue and Organ Procurement ; Tissue Donors ; Transplants ; Waiting Lists