To evaluate the relationship between p53 protein expression and proliferating activity in astrocytic tumors, we performed a study using 37 cases of astrocytic tumors; 13 cases of low-grade astrocytoma (LGA), 9 cases of anaplastic astrocytoma (ANA), and 15 cases of glioblastoma multiforme (GM). The p53 protein expression was studied by immunohistochemical staining (IHC) with DO-7 monoclonal antibody in 37 cases and p53 mutation was detected by single strand conformational polymorphism (SSCP) using PCR products of 31 cases. Proliferating activities were detected by Ki-67 (MIB-1) and proliferating cell nuclear antigen (PCNA). Immunohistochemically, 24.3% (9/37) of astrocytic neoplasms showed p53 expression, which consisted of 7.7% (1/13) of LGA, 44.4% (4/9) of ANA, and 26.7% (4/15) of GM. The p53 expression was statistically significant between the tumor grades. p53 mutations on exon 5 were noted in 6 (19.4%) out of 31 cases of astrocytic tumors. Average indices of MIB-1 and PCNA were 1.5 2.6% and 7.0 10.1% in LGA, 10.0 12.7% and 23.7 23.2% in ANA, and 30.9 22.4% and 69.9 26.7% in GM, respectively. p53 positive group by IHC showed significantly higher average MIB-1 (26.2 23.5%) and PCNA index (56.7 30.3%) than those (12.1 18.3%, 27.6 29.6%) of p53 negative group (p<0.05). p53 mutation group also showed significantly higher MIB-1 (30.7 26.0%) and PCNA index (55.5 32.6%) than those without p53 mutation (10.8 16.5%, 24.2 28.7% respectively). These results showed that about one-fifth of astrocytic tumors have p53 abnormalities, which were related with higher proliferating activities than those without p53 abnormalities.
Astrocytoma ; Exons ; Glioblastoma ; Immunohistochemistry ; Polymerase Chain Reaction ; Proliferating Cell Nuclear Antigen

No abstract available.
Animals ; Cardioplegic Solutions* ; Oxygen* ; Rats*

No abstract available.
Humans ; Pneumothorax*

No abstract available.
Heart Diseases* ; Heart*

Since the discovery of U. urealyticum in human. the etiologic role of this microbe to urogenital tract infection has been investigated. U. urealyticum is one of the etiologic agents for such infections of human urogenital tract as nongonococcal urethritis. prostatitis. cystitis. infertility. interauterine infection, in which standard bacteriologic investigation had failed to indicate bacterial infection. We studied the occurrence of U. urealyticum in urogenital tract of persons in the district of Pusan. From March, 1990 to June. 1990, 290 heterosexual patients and 102 normal control were cultured for U. urealyticum. The results were as follows : l. Overall incidence of this organism was 19.3%, 32.2% from urethral swab and 10.5% from urine, which was lower than that of control group. 2. The incidence of this organism in Females (32.7%) was generally higher than that in males ( 16.6%). 3. This microbe was well detected in middle age person. 4. This microbe was higher detected in specimen from urethral swab (32.2%) than urine (10.5%). 5. The isolation ratio in local private clinics was lower than that in Kosin medical center. 6. Erythromycin had the best sensitivity against U. urealyticum followed by streptomycin. But tetracycline and oxytetacycline were highly resistant to this organism.
Bacterial Infections ; Busan ; Cystitis ; Erythromycin ; Female ; Heterosexuality ; Humans ; Incidence ; Infertility ; Male ; Middle Aged ; Mycoplasma ; Prostatitis ; Streptomycin ; Tetracycline ; Ureaplasma urealyticum* ; Ureaplasma* ; Urethritis

Intravenous pyogenic granuloma (IVPG) is a form of pyogenic granuloma (PG) in which the capillary proliferation is confined entirely within the lumen of a vein. It occurs typically in the neck and upper extremities. Histologically, this benign lesion is similar to PG of other localizations and is characterized by a lobular proliferation of capillaries growing in a sparsely cellular, edematous collagenous stroma. We report a case of IVPG developing in the palm, an unusual site of occurrence.
Capillaries ; Collagen ; Granuloma, Pyogenic* ; Neck ; Upper Extremity ; Veins

There is no single characteristic clinical or radiological feature to diagnose xanthogranulomatous pyelonephritis, so it makes accurate preoperative diagnosis difficult. In our case, the renal parenchyme of affected kidney was well preserved on excretory urogram. The abnormal finding was only a filling defect within the right upper pelvocalyceal system. Herein we report a case of xanthogranulomatous pyelonephritis in a 18 years old girl which was confused with renal pelvic tumor.
Adolescent ; Diagnosis ; Female ; Humans ; Kidney ; Pyelonephritis, Xanthogranulomatous*

One of the complication during or after subclavian vein cannulation is intravascular catheter or wire embolization. Although some studies have reported safety of retaining foreign body embolization, and even death. The intravascular foreign body can be removed surgically or non-surgically. With improvement in instrument technology and technique, percutaneous retrieval of intravascular foreign bodies has become a relatively common procedure. Commonly used methods to remove intravascular foreign bodies are loop snare and basket technique. Sometimes biopy forcep can be used. We have experienced 2 cases of non-surgical removal of intravascular foreign bodies. One of the foreign bodies was 7cm wire fragment in right atrium(RA), the other was a 50cm guide wire. We used the standard loop snare technique for removal of 7cm wire fragment in RA and stone removal basket and 3.0mm ACS PTCA balloon to remove the 50cm short guide wire.
Catheterization ; Catheters ; Foreign Bodies* ; SNARE Proteins ; Subclavian Vein ; Surgical Instruments

No abstract available.
Coronary Artery Bypass* ; Coronary Vessels*

This is a retrospective clinical analysis of 156 patient with colorectal cancer who were surgically treated from January 1988 to June 1996 at the Department of Surgery, Seoul Adventist Hospital. The results are as follows: 1) The peak age incidence was in the 7th decade (31.4% of the cases), and the sex ratio of males to females was 1.03 : 1. 2) The most common location of the tumor was the rectum in 77 cases (49.4%); next were the sigmoid colon in 25 cases (16.0%) and the ascending colon in 25 cases (16.0%). 3) In the right colon, the most frequent symptoms and signs were abdominal pain, a palpable mass, weight loss; in the left colon and rectum, bloody tarry stool and bowel- habit change were the most common symptoms and signs. 4) The duration of the symptoms and signs prior to admission was most commonly less than 3 month (46.8% of the cases). 5) The diagnostic methods were digital rectal examination, sigmoidoscopy, colonofiberoscopy, barium enema, and abdominal CT. In two cases,an exploratory laparotomy was done. Also, 2.8 studies were done per patient. 6) The operations performed included an abdominoperineal resection in 36 cases (24.0%) and a right hemicolectomy (18.7%). The operability was 96.2%, and the total resectability was 79.5%. 7) The staging of the tumor was performed during the initial operation according to the Aster Coller classification and the TNM classification. Stages C2 (33.8%) and B2 (29.1%) and T3N0M0 were the most frequent stages in both classification. 8) The most common histologic type was an adenocarcinoma (96.8%). 9) The most common macroscopic finding was of the annular type (59.6%) 10) The most common distant metastasis sites were the pelvic organs and the liver. 11) The most frequent postoperative complication was wound infection (14.7%). The complication rate and perioperative mortality were 32.7% and 2%, respectively.
Abdominal Pain ; Adenocarcinoma ; Barium ; Classification ; Colon ; Colon, Ascending ; Colon, Sigmoid ; Colonic Neoplasms ; Colorectal Neoplasms* ; Digital Rectal Examination ; Enema ; Female ; Humans ; Incidence ; Laparotomy ; Liver ; Male ; Mortality ; Neoplasm Metastasis ; Postoperative Complications ; Rectal Neoplasms ; Rectum ; Retrospective Studies ; Seoul ; Sex Ratio ; Sigmoidoscopy ; Tomography, X-Ray Computed ; Weight Loss ; Wound Infection