To evaluate the relationship between p53 protein expression and proliferating activity in astrocytic tumors, we performed a study using 37 cases of astrocytic tumors; 13 cases of low-grade astrocytoma (LGA), 9 cases of anaplastic astrocytoma (ANA), and 15 cases of glioblastoma multiforme (GM). The p53 protein expression was studied by immunohistochemical staining (IHC) with DO-7 monoclonal antibody in 37 cases and p53 mutation was detected by single strand conformational polymorphism (SSCP) using PCR products of 31 cases. Proliferating activities were detected by Ki-67 (MIB-1) and proliferating cell nuclear antigen (PCNA). Immunohistochemically, 24.3% (9/37) of astrocytic neoplasms showed p53 expression, which consisted of 7.7% (1/13) of LGA, 44.4% (4/9) of ANA, and 26.7% (4/15) of GM. The p53 expression was statistically significant between the tumor grades. p53 mutations on exon 5 were noted in 6 (19.4%) out of 31 cases of astrocytic tumors. Average indices of MIB-1 and PCNA were 1.5 2.6% and 7.0 10.1% in LGA, 10.0 12.7% and 23.7 23.2% in ANA, and 30.9 22.4% and 69.9 26.7% in GM, respectively. p53 positive group by IHC showed significantly higher average MIB-1 (26.2 23.5%) and PCNA index (56.7 30.3%) than those (12.1 18.3%, 27.6 29.6%) of p53 negative group (p<0.05). p53 mutation group also showed significantly higher MIB-1 (30.7 26.0%) and PCNA index (55.5 32.6%) than those without p53 mutation (10.8 16.5%, 24.2 28.7% respectively). These results showed that about one-fifth of astrocytic tumors have p53 abnormalities, which were related with higher proliferating activities than those without p53 abnormalities.
Astrocytoma ; Exons ; Glioblastoma ; Immunohistochemistry ; Polymerase Chain Reaction ; Proliferating Cell Nuclear Antigen

No abstract available.
Animals ; Cardioplegic Solutions* ; Oxygen* ; Rats*

No abstract available.
Heart Diseases* ; Heart*

No abstract available.
Humans ; Pneumothorax*

Since the discovery of U. urealyticum in human. the etiologic role of this microbe to urogenital tract infection has been investigated. U. urealyticum is one of the etiologic agents for such infections of human urogenital tract as nongonococcal urethritis. prostatitis. cystitis. infertility. interauterine infection, in which standard bacteriologic investigation had failed to indicate bacterial infection. We studied the occurrence of U. urealyticum in urogenital tract of persons in the district of Pusan. From March, 1990 to June. 1990, 290 heterosexual patients and 102 normal control were cultured for U. urealyticum. The results were as follows : l. Overall incidence of this organism was 19.3%, 32.2% from urethral swab and 10.5% from urine, which was lower than that of control group. 2. The incidence of this organism in Females (32.7%) was generally higher than that in males ( 16.6%). 3. This microbe was well detected in middle age person. 4. This microbe was higher detected in specimen from urethral swab (32.2%) than urine (10.5%). 5. The isolation ratio in local private clinics was lower than that in Kosin medical center. 6. Erythromycin had the best sensitivity against U. urealyticum followed by streptomycin. But tetracycline and oxytetacycline were highly resistant to this organism.
Bacterial Infections ; Busan ; Cystitis ; Erythromycin ; Female ; Heterosexuality ; Humans ; Incidence ; Infertility ; Male ; Middle Aged ; Mycoplasma ; Prostatitis ; Streptomycin ; Tetracycline ; Ureaplasma urealyticum* ; Ureaplasma* ; Urethritis

Hemorrhagic cystitis remains a significant complication and morbidity after local radiation therapy to the pelvic region despite close attention to various preventive measures and it was poor response to multi-modality therapies. We have analyzed some factors affecting treatment of radiation hemorrhagic cystitis. 2,238 patients were admitted to the Department of Urology. Kosin Medical College, Pusan, Korea between May. 1985 and February, 1990. Among them 28(1.3%) cases were diagnosed in radiation hemorrhagic cystitis. The most common cause for radiation therapy was uterine cervical cancer(92.9%). The major symptoms were gross hematuria, frequency, nocturia, dysuria and nausea. Under the cystoscopy, the bladder mucosa was seen hyperemia, telangiectasia, mucosal edema and focal necrosis. The combined disease was 4 cases, radiation colitis in 2 cases, radiation proctitis in 1 case and neurogenic bladder in 1 case. There was statistical significant in recurrence rate between receive more than 4,600 rad and less than 4.600 rad (p<0.05). And there was statistically significant in consumption time for treatment between combined with urinary tract infection (41.7 %) and not combined with it (31.3 %)(p<0.05). The patients were controlled with cold saline irrigation, intravesical formalin instillation and hyperbaric oxygen therapy.
Busan ; Colitis ; Cystitis* ; Cystoscopy ; Dysuria ; Edema ; Formaldehyde ; Hematuria ; Humans ; Hyperbaric Oxygenation ; Hyperemia ; Korea ; Mucous Membrane ; Nausea ; Necrosis ; Nocturia ; Pelvis ; Proctitis ; Recurrence ; Telangiectasis ; Urinary Bladder ; Urinary Bladder, Neurogenic ; Urinary Tract Infections ; Urology

There is no single characteristic clinical or radiological feature to diagnose xanthogranulomatous pyelonephritis, so it makes accurate preoperative diagnosis difficult. In our case, the renal parenchyme of affected kidney was well preserved on excretory urogram. The abnormal finding was only a filling defect within the right upper pelvocalyceal system. Herein we report a case of xanthogranulomatous pyelonephritis in a 18 years old girl which was confused with renal pelvic tumor.
Adolescent ; Diagnosis ; Female ; Humans ; Kidney ; Pyelonephritis, Xanthogranulomatous*

Intravenous pyogenic granuloma (IVPG) is a form of pyogenic granuloma (PG) in which the capillary proliferation is confined entirely within the lumen of a vein. It occurs typically in the neck and upper extremities. Histologically, this benign lesion is similar to PG of other localizations and is characterized by a lobular proliferation of capillaries growing in a sparsely cellular, edematous collagenous stroma. We report a case of IVPG developing in the palm, an unusual site of occurrence.
Capillaries ; Collagen ; Granuloma, Pyogenic* ; Neck ; Upper Extremity ; Veins

No abstract available.
Coronary Artery Bypass* ; Coronary Vessels*

One of the complication during or after subclavian vein cannulation is intravascular catheter or wire embolization. Although some studies have reported safety of retaining foreign body embolization, and even death. The intravascular foreign body can be removed surgically or non-surgically. With improvement in instrument technology and technique, percutaneous retrieval of intravascular foreign bodies has become a relatively common procedure. Commonly used methods to remove intravascular foreign bodies are loop snare and basket technique. Sometimes biopy forcep can be used. We have experienced 2 cases of non-surgical removal of intravascular foreign bodies. One of the foreign bodies was 7cm wire fragment in right atrium(RA), the other was a 50cm guide wire. We used the standard loop snare technique for removal of 7cm wire fragment in RA and stone removal basket and 3.0mm ACS PTCA balloon to remove the 50cm short guide wire.
Catheterization ; Catheters ; Foreign Bodies* ; SNARE Proteins ; Subclavian Vein ; Surgical Instruments