Nuclear morphology and size are important in the diagnosis and classification of non-Hodgkin's lymphoma. The recognition of morphologic features of the lymphoma cells and their interpretation are somewhat subjective and often difficult. We apply the morphometric study in touch imprints from 22 cases of non-Hodgkin's lymphoma classified by Working formulation. Determination of the exact size of the tumor cells and substantial diagnostic value of this method compared with histology are proposed. Morphometric parameters, including nuclear area, perimeter, maximal diameter (D-max), diameter of an equivalent circle for a measured area (D-circle), circularity factor (From PE) and regularity factor (Form AR) are measured, using Kontron, user-controlled image analyser (IBAS-1). The correlation between morphometric and histologic diagnosis is relatively good, except for 2 cases of Burkitt's lymphoma, measured as large cell and 2 cases of large cell lymphoma measured as medium cell. The most reliable parameter of the nuclear size is D-circle. The parameters for the nuclear shape, Form PE and Form AR, also reflect the nuclear cleavage and polymorphous pattern. The large cell group shows more wide standard deviation than small and medium cell groups, reflecting heterogeneity and variability in unclear size of large cell group. We suggests the morphometric analysis using touch imprints is complementary diagnostic tool for more accurate and reproducible diagnosis.

The Parasitological investigation on the encysted metacercariae in brackish water fish, Tribolodon taczanowskii Steindachner , in the downstream of Hyungsan river which is located in Kyungpook province of Korea, were carried out, and the following results were obtained. Metacercariae of Metagonimus species were found in seventy five fishes (40.5 percent) out of 185 examined. Parasitic frequencies of the encysted matacercaria of Metagonimus species in Tribolodon taczanowskii were 23.2 percent (43 out of 185) in the scale, 7.0 percent (13 out of 185) in the gill, and 10.3 percent (19 out of 185) in the flesh. The number of the metacercaria in the scale, gill and flesh were fewer than that of the others. The worms were identified as Metagonimus yokogawai Katsurada, 1912 by morphological studies on the adults and eggs, and compared with prereported Metagonimus species.
parasitology-helminth-trematoda ; Metagonimus yokogawai ; epidemiology ; Tribolodon taczanowskii

Lobular carcinoma of the male breast is very rare, because of the absence of lobules in the normal male breast. Herein, a case of lobular carcinoma of the male breast with cellular features of signet ring cells is described. A 57-year-old man presented with a left breast mass. Histologic examination showed classic invasive lobular carcinoma with in situ component. Most infiltrating tumor cells had a prominent signet ring cell appearance. The patient was phenotypically male and had fathered children. There was no history of predisposing factors to breast lesion, such as hormone use or gynecomastia.
Breast* ; Carcinoma, Lobular* ; Causality ; Child ; Fathers ; Gynecomastia ; Humans ; Male* ; Middle Aged

Lobular carcinoma of the male breast is very rare, because of the absence of lobules in the normal male breast. Herein, a case of lobular carcinoma of the male breast with cellular features of signet ring cells is described. A 57-year-old man presented with a left breast mass. Histologic examination showed classic invasive lobular carcinoma with in situ component. Most infiltrating tumor cells had a prominent signet ring cell appearance. The patient was phenotypically male and had fathered children. There was no history of predisposing factors to breast lesion, such as hormone use or gynecomastia.
Breast* ; Carcinoma, Lobular* ; Causality ; Child ; Fathers ; Gynecomastia ; Humans ; Male* ; Middle Aged

No abstract available.
Adenocarcinoma* ; alpha-Fetoproteins*

A newly developed third generation enzyme immunoassay(Lucky HCD 3.0 EIA) for hepatitis C virus(HCV) antibodies was added with the envelope(E1E2)/NS4 fusion proteins and expanded NS5 proteins as well as the core/NS3 fusion proteins. Authors evaluated the HCD 3.0 EIA with the previously available second generation EIA(HCD 2.0) in 10,435 Red Cross blood donors. Among 10,435 donors who were screened for the presence of HCV antibodies by HCD 2.0 assay, 22(0.21%) sera were repeatedly reactive. All of these sera were tested for further testing. Only 13 of all tested sera were reactive by HCD 3.0 EIA, and nine sera were not reactive. Nine of 13 HCD 3.0 positive sera were reactive by recombinant immunoblot assay(Lucky-Confirm). Also seven of these 13 sera had detectable HCV genomic RNA by reverse transcriptase-polymerase chain reaction(RT-PCR). None of nine HCD 3.0 negative samples had detectable immunoblot assay and HCV genomic RNA. It is concluded that the new HCV EIA can decrease a significant false positivity of second generation EIA in a blood donor population. This new assay correlates well with detection of HCV-RNA by RT-PCR and identifies donors who are truly infected.
Antibodies ; Blood Donors* ; Hepatitis C ; Hepatitis C Antibodies ; Humans ; Immunoblotting* ; Red Cross ; RNA ; Tissue Donors

Chemical rhizotomy with the intrathecal alcohol injection was firstly attempted by Dogliotti in 1931, and with phenol in 1955 by Maher. The intrathecal neurolytic injection was a very simple, effective and nonsurgical procedure without any great danger. Good indications for the procedure are the bilateral pelvic and lower limb pain syndrome, well circumscribed pain in limited area and in poor general condition. We have reported two cases of intractable pain of limited area, successfully relieved by chemical rhizotomy, and discussed the importance of the procedure and presented the review of literatures.
Lower Extremity ; Pain, Intractable* ; Phenol ; Rhizotomy

In CAPD patients, exit site infection and peritonitis are occasionally not improved due to undetected tunnel infection. The diagnosis of tunnel infection is mainly based on the clinical symptoms and signs which seem insensitive for early diagnosis and timely intervention. We examined usefulness of catheter ultrasonography(USG) as a tool in the diagnosis of tunnel infection in various kinds of infectious complications in CAPD patients. Positive result was defined by the presence of hypoechoic area more than 2mm in diameter along the subcutaneous catheter tunnel. 26 USG were performed in 23 patients who represented normal in 4 cases, exit site infections in 6 cases, tunnel infections in 2 cases, peritonitis in 10 cases and 4 cases of combined infections. The mean age was 53.6 years and mean duration of CAPD and break-in period were 16.7 months and 24.7 days respectively. Positive USG findings were noted in 13 cases(50%). High rate of positive finding was observed in clinically suspected exit site infection (80.3%), tunnel infection(100%) and combined infections (75%). Positive results were most commonly observed near the external cuff area. The most common causative organism was S. aureus followed by Pseudomonas and CNS infections. S. aureus infection most likely resulted in positive sonographic findings and catheter loss despite of antibiotic treatment. We conclude that USG is a sensitive tool for the diagnosis of tunnel infections and tunnel infection caused by S. aureus is at high risk of catheter loss.
Catheters* ; Diagnosis* ; Early Diagnosis ; Humans ; Peritoneal Dialysis, Continuous Ambulatory* ; Peritonitis ; Pseudomonas ; Ultrasonography*

In CAPD patients, exit site infection and peritonitis are occasionally not improved due to undetected tunnel infection. The diagnosis of tunnel infection is mainly based on the clinical symptoms and signs which seem insensitive for early diagnosis and timely intervention. We examined usefulness of catheter ultrasonography(USG) as a tool in the diagnosis of tunnel infection in various kinds of infectious complications in CAPD patients. Positive result was defined by the presence of hypoechoic area more than 2mm in diameter along the subcutaneous catheter tunnel. 26 USG were performed in 23 patients who represented normal in 4 cases, exit site infections in 6 cases, tunnel infections in 2 cases, peritonitis in 10 cases and 4 cases of combined infections. The mean age was 53.6 years and mean duration of CAPD and break-in period were 16.7 months and 24.7 days respectively. Positive USG findings were noted in 13 cases(50%). High rate of positive finding was observed in clinically suspected exit site infection (80.3%), tunnel infection(100%) and combined infections (75%). Positive results were most commonly observed near the external cuff area. The most common causative organism was S. aureus followed by Pseudomonas and CNS infections. S. aureus infection most likely resulted in positive sonographic findings and catheter loss despite of antibiotic treatment. We conclude that USG is a sensitive tool for the diagnosis of tunnel infections and tunnel infection caused by S. aureus is at high risk of catheter loss.
Catheters* ; Diagnosis* ; Early Diagnosis ; Humans ; Peritoneal Dialysis, Continuous Ambulatory* ; Peritonitis ; Pseudomonas ; Ultrasonography*

BACKGROUND: The aims of this study were to compare the cytomorphologic features diagnostic of atypical squamous cells (ASC) in liquid-based preparations (LBPs) and conventional Pap (CP) smears and to cytomorphologically assess the performance of the Cell Scan 1500(TM) in cervical cytology practice. METHODS: Cervicovaginal smears were obtained from 938 women. Two smears were obtained simultaneously from each individual, one for an LBP and the other for a CP smear; the smears were independently examined. ASC was diagnosed in 24 patients, and their samples were cytomorphologically and semiquantitatively analyzed. RESULTS: A total of 24 of the 938 women (2.6%) were diagnosed with ASC by one or both methods. Results from LBPs and CP smears were in agreement in 13 of 24 cases of ASC diagnosis (absolute direct agreement, 54.2%; k<0.20; p-value from chi-square test=0.085). Diagnostic features of ASC in the LBPs included squamous cell atypia and atypical squamous metaplasia. CONCLUSIONS: The cellular features diagnostic of ASC present in one preparation can manifest themselves differently in the other. Changes in individual cells, particularly nuclear changes, are the most reliable features for diagnosing ASC. The Cell Scan 1500(TM) processor is more effective at detecting ASC than are CP smears.
Female ; Humans