1.Arthroscopic Study of the Separated Suprapatellar Bursa
Byung Ill LEE ; Soo Kyoon RAH ; Chang Uk CHOI ; Yon Il KIM ; Jong Cheol LEE
The Journal of the Korean Orthopaedic Association 1985;20(5):913-918
A clinical application of the arthroscopy to the three hundred symptomatic knees were performed, and seven knees with a symptomatic seprated suprapatellar bursa were successfully managed and followed by arthroscopic surgery at the Department of Orthopaedic Surgery, Soon Chun Hyang University Hospital from May 1983 to May 1985. The results obtained are as follows; 1. Separated suprapatellar bursa may cause some internal derangement of the knee. 2. The incidence of the separated suprapatella bursa in our view was 5% that is, of 300 symptomatic knees. 3. Separated suprapatellar bursa may have to be excised under arthroscopy.
Arthroscopy
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Incidence
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Knee
2.Labeling Dual Presynaptic Inputs using cFork Anterograde Tracing System
Jun-Young OH ; Jeong-Ho HAN ; Hyoeun LEE ; Young-Eun HAN ; Jong Cheol RAH ; Hyungju PARK
Experimental Neurobiology 2020;29(3):219-229
Understanding brain function-related neural circuit connectivity is essential for investigating how cognitive functions are decoded in neural circuits. Trans-synaptic viral vectors are useful for identifying neural synaptic connectivity because of their ability to be transferred from transduced cells to synaptically connected cells. However, concurrent labeling of multisynaptic inputs to postsynaptic neurons is impossible with currently available trans-synaptic viral vectors. Here, we report a neural circuit tracing system that can simultaneously label postsynaptic neurons with two different markers, the expression of which is defined by presynaptic input connectivity. This system, called “cFork (see fork)”, includes delivering serotype 1-packaged AAV vectors (AAV1s) containing Cre or flippase recombinase (FlpO) into two different presynaptic brain areas, and AAV5 with a dual gene expression cassette in postsynaptic neurons. Our in vitro and in vivo tests showed that selective expression of two different fluorescence proteins, EGFP and mScarlet, in postsynaptic neurons could be achieved by AAV1-mediated anterograde trans-synaptic transfer of Cre or FlpO constructs. When this tracing system was applied to the somatosensory barrel field cortex (S1BF) or striatum innervated by multiple presynaptic inputs, postsynaptic neurons defined by presynaptic inputs were simultaneously labeled with EGFP or mScarlet. Our new anterograde tracing tool may be useful for elucidating the complex multisynaptic connectivity of postsynaptic neurons regulating diverse brain functions.