Background: E-scooter usage was lawfully approved in Germany in June 2019. Since then, a marked increase of e-scooter drivers has been noticed. Evidence concerning factors that may affect the severity of these injuries is limited. The study aimed to retrospectively analyze e-scooter-related injuries in a major German city. Methods: All patients admitted to the emergency department of a level I trauma center in Berlin, Germany, between June 15, 2019, and December 15, 2019, were retrospectively reviewed. Patients involved in an e-scooter accident were included in this study, and their medical reports were analyzed. Results: In the study period, 43 patients were involved in an e-scooter accident and could be included in this study. The median age of the patients was 30 years (interquartile range [IQR], 24.50–39.50 years), with 19 (44.2%) being female patients. The median Injury Severity Score of all patients was 2.0, with the highest Abbreviated Injury Scale (AIS) of 3.00 (IQR, 2.00–3.00) recorded as thoracic injuries. Seven patients had extremity fractures, of which 4 had to be stabilized operatively. In 12 patients (27.9%), the accidents occurred under the influence of alcohol. Conclusions The majority of injuries reported in this study were associated with a relatively low AIS, possibly due to strict local speed limits. Nonetheless, e-scooter usage bears risks of sustaining severe injuries to the head, face, and extremities, particularly under the influence of alcohol or when illegally ignoring local laws.