Purpose: The advent of proteomics provides new opportunities to investigate the molecular mechanisms underlying male infertility. The selection of relevant targets based on a single analysis is not always feasible, due to the growing number of proteomic studies with conflicting results. Thus, this study aimed to systematically review investigations comparing the sperm proteome of normozoospermic and infertile men to define a panel of proteins with the potential to be used to evaluate sperm quality. Materials and Methods: A literature search was conducted on PubMed, Web of Science, and Scopus databases following the PRISMA guidelines. To identify proteins systematically reported, first the studies were divided by condition into four groups (asthenozoospermia, low motility, unexplained infertility, and infertility related to risk factors) and then, all studies were analysed simultaneously (poor sperm quality). To gain molecular insights regarding identified proteins, additional searches were performed within the Human Protein Atlas, Mouse Genome Informatics, UniProt, and PubMed databases. Results: Thirty-two studies were included and divided into 4 sub-analysis groups. A total of 2752 proteins were collected, of which 38, 1, 3 and 2 were indicated as potential markers for asthenozoospermia, low motility, unexplained infertility and infertility related to risk factors, respectively, and 58 for poor sperm quality. Among the identified proteins, ACR, ACRBP, ACRV1, ACTL9, AKAP4, ATG3, CCT2, CFAP276, CFAP52, FAM209A, GGH, HPRT1, LYZL4, PRDX6, PRSS37, REEP6, ROPN1B, SPACA3, SOD1, SPEM1, SPESP1, SPINK2, TEKT5, and ZPBP were highlighted due to their roles in male reproductive tissues, association with infertility phenotypes or participation in specific biological functions in spermatozoa. Conclusions Sperm proteomics allows the identification of protein markers with the potential to overcome limitations in male infertility diagnosis and to understand changes in sperm function at the molecular level. This study provides a reliable list of systematically reported proteins that could be potential targets for further basic and clinical studies.

BACKGROUND AND AIM: A healthy work environment is one in which workers and managers cooperate in the process of continuous improvement regarding the protection, and promotion of workers' health and well-being, for the sake of work sustainability. The current state of the art shows that an unfavourable work environment contributes to nurses' dissatisfaction, burnout and emotional exhaustion, and the intention to leave the workplace/service. This study aimed to identify the Nurses' Work Environment in a small Portuguese Hospital, diagnose the situation, and focus on a healthy work environment. METHODS AND MATERIALS: This is a quantitative, descriptive, and cross-sectional study, with a convenience sample of 90 nurses. The “Escala de Ambiente de Trabalho da Prática de Enfermagem” (Nursing Practice Work Environment Scale), validated and adapted by Ferreira & Amendoeira (2014) for the Portuguese population, was applied. The statistical treatment was performed using SPSS 27. RESULTS: The sample is mostly composed of female nurses (87%) with an average age of 26 years, and 75.5% are single. The dimension "Management and Leadership of the Head Nurse" had a mean value of x̅ =3.3 (out of 4) and σ=0.5, which was the dimension with the best assessment. The dimension with less encouraging results was "Nurses' Participation in Hospital Affairs" with x̅ =2.8 (out of 4) and σ=0.7. Overall, all domains assessed had a mean value above 2.5, which was considered a favourable work environment by the nurses. CONCLUSION The results, although generally satisfactory, show the need to carefully intervene and assess each dimension in an integrated perspective, to promote a healthy work environment and workers' well-being, since its imbalance may negatively affect quality of their work, impacting the quality of health care provided to the user . In addition, these results should be understood as an important factor to be considered in the design of future care teams. Regardind future research, it would be important to consider large samples and deepen the topic explored among different departments in the health care facilities.
Nurses ; Workplace ; Health Promotion