1.Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis.
Eun Hee SHIN ; Dong Hee KIM ; Aifen LIN ; Jo Woonyi LEE ; Hyo Jin KIM ; Myoung Hee AHN ; Jong Yil CHAI
The Korean Journal of Parasitology 2008;46(1):45-48
To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
Adolescent
;
Adult
;
Animals
;
Antigens, Protozoan/*blood
;
Enzyme-Linked Immunosorbent Assay
;
Female
;
Humans
;
Korea
;
Latex Fixation Tests
;
Male
;
Mice
;
Middle Aged
;
Rabbits
;
Serologic Tests
;
Toxoplasmosis/blood/*diagnosis
2.Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis.
Eun Hee SHIN ; Dong Hee KIM ; Aifen LIN ; Jo Woonyi LEE ; Hyo Jin KIM ; Myoung Hee AHN ; Jong Yil CHAI
The Korean Journal of Parasitology 2008;46(1):45-48
To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
Adolescent
;
Adult
;
Animals
;
Antigens, Protozoan/*blood
;
Enzyme-Linked Immunosorbent Assay
;
Female
;
Humans
;
Korea
;
Latex Fixation Tests
;
Male
;
Mice
;
Middle Aged
;
Rabbits
;
Serologic Tests
;
Toxoplasmosis/blood/*diagnosis