Periprosthetic joint infection (PJI) is a devastating complication after total joint arthroplasty.Currently,PJI is the main cause for knee revision and the third reason for hip revision.Accurate diagnosis is of quite importance for patients with potential in revision surgery.However,there is no test alone can achieve both perfect sensitivity and specificity in diagnosis of PJI.The following diagnostic methods still have irreplaceable role,including erythrocyte sedimentation rate (ESR),C reactive protein (CRP) test,synovial white blood cell count and polymorphonuclear percentage,bacteria culture and histology analysis.Some novel diagnostic techniques,such as leucocytes esterase strip test,show advantages in diagnosing as fast,convenient and cost-saving.Synovial biomarkers,such as α-defensin,have promising prospect with both high sensitivity and specificity.Among various diagnostic methods,bacteria culture and drug sensitivity test can guide the therapy of PJI.Thus,it is crucial to improve the positive rate of bacteria culture.With the development of technology,sonication and polymerase chain reaction (PCR) will be the future research highlights.Although the imaging technologies,such as labeled leukocyte imaging and 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET),have been progressed in the past years,most of them are still in controversy.Further researches are still needed in the future.

Objective To investigate the application of ureteral catheter in complicated gynecological laparoscopic surgery for the prevention of ureteral injury . Methods A total of 182 cases of gynecological laparoscopic surgery performed from January 2009 to November 2011 were selected as study group .Bilateral ureteral catheters were inserted under cystoscopy , and then laparoscopic surgery was performed under the instruction of the catheter .The catheter was immediately removed after the surgery .A total of 200 cases of laparoscopic surgery were selected as control group and preoperative ureteral catheterization was not applied in this group .The incidence rate of ureteral injury was compared between the two groups . Results The success rate of bilateral ureteral catheterization in the study group was 98.9%(180/182)and no ureteral injury occurred .In the control group, ureteral injury was found in 2 cases, which were converted to open surgery for ureter repair and double J stent was retained .The 2 cases recovered 2 months after the surgery.Bladder injury was found in 2 cases during the surgery , which received immediate repair assisted by surgeons from the department of urinary surgery and recovered 2 months after postoperative catheterization . No statistical difference was found in complication rate between the two groups ( P=0.125 ) . Conclusion The application of ureteral catheterization in complicated gynecological laparoscopic surgery can reduce the risk of intraoperative ureteral injury .

OBJECTIVE:To establish a RP-HPLC method for the determination of the content of chlorhexidine acetate in daobian suppository.METHODS:The determination was performed on Diamonsil C_(18)column,The mobile phase consisted of acetonitrile-water-triethylamine(30:70:0.5,with pH adjusted to 3.0 by phosphoric acid)at a flow rate of 1.0 mL?min~(-1),The detective wavelength was 260 nm,the sample size was 20?L and the column temperature was maintained at 30℃. RESULTS:The linear range of Chlorhexidine Acetate was 1.092～13.10?g?mL~(-1)(r=0.999 9)and its average recovery was 98.29%(RSD=2.16%,n=9).CONCLUSIONS:The method is simple and accurate,and it can be used for the determination of the content of chlorhexidine acetate in daobian suppository.

Objective To discuss the curative effect of arthroscopiclly assisted radiofrequency tenotomy of the sternocleidomastoid muscle for the treatment of congenital muscular torticollis in teenagers. Methods A total of 12 patients (right, 4 patients; left, 8 patients) were enrolled in the study. A 3-mm skin incision was made at the site 5 cm below the sternoclavicular joint. A dissector was inserted through the incision to perform blunt dissection subcutaneously until the attachment points of the sternocleidomastoid muscle. After a subcutaneous operative space 3 cm ? 3 cm in size was created, a 30? wide-angle arthroscope was placed. Another incision was located at the site 5 cm below the mid-clavicle for placing the bipolar radiofrequency probes (Arthrocare 2000). Under the assistance of arthroscopy, the sternocleidomastoid muscle was severed from the attachment at the both sides. Results The operation time was 15~30 min (mean, 20 min). No intraoperative hemorrhage or vascular and nervous injuries were observed. Follow-up for 5~10 months (mean, 7 months) in the 12 patients found normal appearance and no recurrence or diplopia. Conclusions Arthroscopiclly assisted radiofrequncy therapy for congenital muscular torticollis is characterized by simplicity of manipulation, micro-invasion, and satisfactory curative effect.

[Objective]To evaluate the safety outcome of the 12 patients with simultaneous multiple joint replacement(≥3) in the recent three years.[Method]The complication rates,blood loss,transfusion volume,and hospital stay were analyzed in details.[Result]The mean operating time was 9 hours and 8 minutes(6 hours and 25 minutes to 12 hours).The mean overall volume of blood loss was 3140ml(1 000 ml-5 600 ml.Complications included reactive digestive ulcer in one case,acetabular component position error in one case,and deep infection in one hip of one case.[Conclusion]Simultaneous multiple joint replacement is an alternative protocol to the patients with multiple joint disorders.finished under a single aesthesia and in a single hospital stay.So the overall economical cost will be decreased,and rehabilitation being accelerated.Disadvantages include serious trauma,increased blood loss,and complications may be some how increased.

[Objective]To evaluate the clinical outcome of the Ribbed anatomic cementless prosthesis.[Method]A total of 1436 hips in 956 patients undergone THR with Ribbed anatomic cementless prosthesis were reviewed.Between March 1997 and September 2005,1 249 hips in 832 patients were available for clinical and radiological follow-up.The mean follow-up period was 6.7 years with a minimum of 2 and a maximum of 10.5 years.The patients were evaluated with Harris Hip Score and radiographic analysis.[Result]The mean Harris Hip Score increased from 34.7 preoperatively to 91.5 postoperatively.Excellent and good functional improvement was seen in 96.3% of the operated patients.There was neither periprosthetic osteolysis nor evidence of loosening on follow-up radiographs.[Conclusion]Ribbed anatomic cementless prosthesis is found to be performing well with good clinical and radiological result.

Objective To study the application of debridement with arthroscopy in acetabular dysplasia with osteoarthritis. Methods Thirty-two cases in 48 hips acetabular dysplasia with osteoarthritis debrided under the arthroscopy. In this group, male 9 and female 23, the mean age was 38.1 years old (range, 28-52 years old), the left side for 9, right side for 7 and bilateralis for 16. In the 48 hips, X-ray showed that comparatively large acetabular draw with normal position of femoral head in 21 cases (28 hips), large acetabular draw with femoral head lateral shift slightly but did not exceed 1/3 of femoral head in 11 cases (20 hips).MRI demonstrated that intraarticular fluid in 28 hips, subcartilage anormal signal of acetabular and femoral head at weight loading region in 10 hips, signal interruption of glenoid lip of hip in 10 hips. The procdure which included to remove of osteophyma, loose body and hyperplasia synovial membrane, degenerated cartilage on the stress areas of the hip joint, wear and tear of glenoid lip. Results The perianal skin in 2 patients suffered dysesthesia because of the over traction during operation. They recovered 7 days postoperation. Dysesthesias twenty-eight patients (36 hips) were follow-up with an average of 23 months (range, 6 months-3.5 years). After debridement and lavage, the patients released from pain, restored function and the range of the motion. According to VAS evaluation system for pain, the preoperative score was 5-9, with an average of 6.8; the postoperative score was 4-6, with an average of 2. According to Harris hip joint score evaluation system, preoperative score was 58.8?6.15, postoperative score was 89.6?11.3. The excellent in 20, good in 4, fair in 3 and poor in 1. The excellent and good result was up to 85.7%. Conclusion Selectivity debridement and lavage were effective methods for osteoarthritis hip joint acetabular dysplasia. It can delay the developing of course of disease; improve clinical symptom and elevate quality of life.

AIM: To optimize the random amplified polymorphic DNA-Polymerase chain reaction(RAPD-PCR) system of Bupleurum chinense DC by comprehensive scoring method. METHODS: The L_(16)(4~5) orthogonal diagram was applied to studying the effects of different concentrations of Taq polymerase,Mg~(2+),dNTPs,primer and DNA on Bupleurum chinense DC.RAPD-PCR results and to get a suitable PCR system. RESULTS: A suitable RAPD reaction system was developed,i.e.1?Taq polymerase reaction buffer,2.5 mmol/L Mg~(2+),1.5 U Taq DNA polymerase,0.15 mmol/L dNTPs,0.4 ?mol/L primer and 25 ng genomic DNA templates in total 25 ?L reaction solution. CONCLUSION: The results show that the comprehensive scoring method could be used successfully to optimize the RAPD-PCR system.

Objective To study the influence of different root exposure in long-term effect of crown lengthening .Methods To-tally 142 teeths(66 of the front teeths ,46 of premolars teeths ,30 of molars teeths ) of 110 patients collected from August 2010 to October 2012 in stomatological hospital to affiliated zunyi medical college were chosen as research subjects ,then they were randomly divided into the experimental group and the control group .There were 33 anterior teeths ,23 premolar teeths and 15 molar teeths in each group .The experimental group accepted 3 mm root exposure while the control group accepted 4 mm root exposure .The post-operative effect of the two groups was compared .Results The tooth mobility of the anterior cases of control group after 3 months was statistically higher than that of the experimental group (P<0 .05) and the difference of tooth mobility between the two groups in other teeths and times was not statistically significant (P>0 .05) .The bleeding index after 3 months ,the anterior teeths ,premo-lar teeths and molar teeths of the experimental group was statistically higher than that of the control group (P<0 .05) ,while the difference of tooth mobility between the two groups in other times was not statistically significant (P>0 .05) .The postoperative probing difference of depth and attachment loss between the two groups showed no statistically significant (P>0 .05) .Conclusion The root exposure of 3 mm and 4 mm both have a significant effect in crown lengthening .But for the aesthetic point of view ,3 mm should be taken as the root exposure of the anterior cases .

The great changes taking place in the environment of academic library were analyzed from the aspects of networked information environment, knowledge-based user needs and homogenized library resources. The main up-date development characteristics of academic library were described according to the current development situation in foreign and domestic academic libraries as revealed in our investigation, such as e-only of library resources, knowledge-based and embedded information service, associated, semantic and integrated information organization, incorporation of primary information resources into institutional knowledge property management, digital library emerged in scientific data monitoring centers, SoLoMo as the concept for creating the next generation mobile library service. It was pointed out that academic library will become the user-oriented knowledge service center that provides user-driving knowledge service and intelligent knowledge service in an all-inclusive knowledge management manner. It is thus necessary for academic library to attach more importance on the information needs of scientific workers, to strengthen the user-oriented professional work layout, application of information technology, interlibrary coopera-tion and resources-sharing, and to improve the information literacy of librarians.