1.Treatment of pancreatic neuroendocrine tumor with liver metastases
Journal of Clinical Hepatology 2015;31(5):668-670
Pancreatic neuroendocrine tumor (pNET)is a rare type of pancreatic tumors.The incidence of pNET shows a gradually increas-ing trend in recent years.The most common organ of distant metastases is the liver.Surgical resection is still the optimal treatment for resect-able,well -differentiated liver metastases with no evidence of extrahepatic spread.For unresectable patients,a combination of multiple mo-dalities,such as transarterial chemoembolization,radiofrequency ablation,systemic chemotherapy,and molecular targeted therapy,can pro-long the survival time of patients.Liver transplantation should be strictly evaluated on an individual basis.
2.Ethics of the donation after cardiac death
Chinese Journal of Digestive Surgery 2013;12(9):644-647
Donation after cardiac death (DCD) means organ harvest and donation cannot be carried on until the requirement of death criteria using cardiopulmonary arrest is reached.Great success has been achieved since DCD was promoted widely in China.However,some problems were encountered during the implementation of DCD and there also exists many ethical controversies.The ethical principles of informed consent and no harm mentioned inDonation after Cardiac Death Work Instructions of China should be strictly adhered to,while justice,fairness and openness must be ensured in organ allocation.Appropriate compensation should be given to DCD donor and his family,and the utilization of DCD should be further improved.All of these ethical principles will help to promote the healthy development of organ donation and transplantation in China.
3.Periesophagogastric devascularization without splenectomy in the treatment of portal hypertension
Chinese Journal of Digestive Surgery 2013;12(11):820-822
Gastroesophageal variceal bleeding is one of the major causes of death for patients with portal hypertension.The treatment of portal hypertension has evolved from surgery being the only option to the wide range of options currently available,including endoscopic sclerotherapy of varietals ligation,interventional radiology and surgery.Given the shortage of liver transplantation donors,devascularization would be one of the major treatments for portal hypertension for a considerable time.Based on the clinical experience of the People's Hospital of Peking University,we think that peraesophagogastric devascularization without splenectomy is a better option for the treatment of portal hypertension,with less operation time,less intraoperative blood loss and fewer postoperative complications,and better short-and long-term hemostatic effect.
4.Surgery for cirrhotic portal hypertension in the era of liver transplantation
Chinese Journal of Hepatobiliary Surgery 2021;27(1):4-7
Liver transplantation is the only curative treatment for cirrhotic portal hypertension, especially for the patients with end-stage liver failure. In the era of liver transplantation, when drugs, endoscopy and interventional treatments fail, surgery (not including liver transplantation) is an irreplaceable treatment option for esophagogastric variceal bleeding, which should be individualized, in order not to increase the risk and difficulty of liver transplantation in the future.
5.The effect of Shenfu injection on ischemia reperfusion injury of rat liver graft
Weihua ZHU ; Xisheng LENG ; Jiye ZHU
Chinese Journal of General Surgery 2001;0(08):-
Objective To investigate the effect of Shenfu injection on ischemia reperfusion injury of rat liver graft. Methods Male SD rats were used as isogeneic orthotopic liver transplantation. Sixty rats were randomly divided into two groups(30 in each). The recipient received Shenfu injection intravenously immediately before the removal of the liver in SF group and normal saline of the same volume in control group. At 3、6 and 24h after the reperfusion blood and hepatic tissue were sampled for examination. Results The levels of superoxide dismutase (SOD) and nitric oxide (NO) in SF group significantly increased compared with those in control group(P
6.Management of early immunosuppressant-associated hypertension after liver transplantation
Fengxue ZHU ; Jiye ZHU ; Guangming LI
Chinese Journal of General Surgery 1993;0(02):-
ObjectiveTo manage the early immunosuppressant-associated hypertention in liver transplantation patients. MethodsFifty-six liver transplantations were performed from May 2000 to May 2002, their clinical feature (data of blood pressure and treatment) were analysed. ResultThe incidence of hypertension after liver transplantation was 70% in 2 weeks, 66% in one month. The incidence in cyclosporine group and FK506 group was 73% (31/40) and 33%(3/9) respectively(P
7.Induction of immunotolerance in mouse pancreatic islet transplantation through PD-1/PD-L1 costimulatory pathway
Tao LI ; Jiye ZHU ; Xisheng LENG
Chinese Journal of General Surgery 2009;24(1):45-48
Objective To investigate the role of recombinant adenovirus Ad-PD-L1 on immunotolerance induction in mouse pancreatic islet transplantation. Methods Full-length mouse PD-L1 cDNA linked with an internal ribosome entry site (IRES)-GFP cassette was subcloned into pShuttle-GFP-CMV( - ) shuttle plasmid. The product was cut by certain restriction endonuclease and ligate with pAdxsi vector. The adenovirus bone plasmid was transformed into DH5α competent bacteria. The positive clone was identified by restriction endonuclease digestion and further confirmed by sequencing. After linearization, the recombined adenovirus DNA was transfected into 293 cells by liposome for package and amplification, which was purified by CsC1 density gradient centrifugation. Streptozocin was injected i.p. into C57BL/6 (H-2b) mouse to induce diabetic model recipient. Recipients were randomly divided into three groups, Group A was the control. Group B and group C were injected of Ad-EGFP and Ad-PD-L1 through tail vein respectively 1 day before islet transplantation. 300 to 400 islets of DBA/2 (H-2d) were transplanted into the renal subcapsular space of the diabetic model recipient. The level of blood sugar and the graft survival time were monitored. Results Recombinant adenovirns Ad-PD-L1 have high efficiency expression of PD-L1 in recipient mouse. The survival time of grafts of Ad-PD-L1 group (27.63 ± 3. 51 ) d was significantly longer than that of the control ( 7. 85 ± 0. 33 ) d and Ad-EGFP group ( 7. 67 ± 0. 59 ) d ( P < 0. 01 ). Mixed lymphocyte response showed a specific decrease reaction of recipient lymphocyte toward donor lymphocytes. Conclusion Recombinant adenovirus Ad-PD-L1 was successfully constructed. In mouse pancreatic islet transplantation, it can suppresses the activation of recipient T lymphocyte through PD-1/PD-L1 co-stimulatory pathway, and significantly prolong the survival time of grafts.
8.The expression of mitogen-activated protein kinase and its upstream regulated signal in human hepatocellular carcinoma
Guangming LI ; Jiye ZHU ; Yannan LIU
Chinese Journal of General Surgery 1997;0(06):-
Objective This study was to detect protein expression of ERK 1,ERK 2,JNK 1,p38 and MEK 1 ,MEK 2 in human hepatocellular carcinoma and adjacent non neoplastic liver tissues.Method Western blot was used to detect the expression of ERK 1,ERK 2, JNK 1,p38 and MEK 1,MEK 2 in the surgically resected hepatocellular carcinoma and para carcinoma tissues in 16 HCC patients.[WT5”HZ] Result The expression of ERK 1,ERK 2,p38 expressed by integral optic density (IOD) in hepatocellular carcinoma was significantly higher than that in para carcinoma respectively: as for ERK 1 it was 300?98 in carcinoma and 98?48 in para carcinoma tissues ( P
9.Evaluation of portal vein pressure by radionuclide imaging in cirrhotic patients undergoing portacaval shunt or pericardial devascularization
Jie GAO ; Jiye ZHU ; Xisheng LENG
Chinese Journal of General Surgery 1993;0(01):-
ObjectiveTo evaluate the changes of portal vein pressure by (PVP) radionuclide imaging in cirrhotic patients undergoing portacaval shunt or esophageal transection-splenectomy.MethodThe radionuclide imaging was used to calculate portal pressure perioperatively in 15 shunt and 20 esophageal transection-splenectomy patients of portal hypertension.Results were compared with direct portal vein manometry.ResultPVP by manometry in portal hypertension patients 〔(37?4)?cm?H 2O〕 was very close to that calculated by preoperative imaging 〔(36?4)?cm?H 2O〕r=0.81,P
10.Surgical treatment of primary retroperitoneal tumor: report of 42 cases
Shu LI ; Maochun WANG ; Jiye ZHU
Chinese Journal of General Surgery 1994;0(05):-
ObjectiveTo study the diagnosis and treatment of primary retroperitoneal tumor ( PRT) and the key for preventing and treating of recurrent retroperitoneal tumor (PRT).MethodsThe clinical data of 42 patients with PRT operated on from 1990 to 2000 were retrospectively analyzed.ResultTumors were benign in 19 patients and malignant in 23. Of the 19 patients with benign tumor, 15 underwent complete resection with incidental organ resection in 3 cases, palliative resection in 1 case, and complete resection of recurrent tumor in one. Of the 23 malignant tumors, complete resection was performed in 9 cases, complete resection with incidental organs resection in 11 cases, biopsy only in 3 cases. There was no operative death.ConclusionPreoperative imaging results are gists on which the resectability is judged, and complete resection is the only hope of cure. Surgical resection is the therapy of choice for postoperative recurrent PRT.