1.Chronic psychological stress and immunity
Chinese Journal of Immunology 2017;33(7):961-966
With the rapid development of our country,people are facing more and more psychological problems.It has been demonstrated that persistent stress and depression,which leads to continuously elevated levels of stress hormones,might eventually compromise host defenses against bacterial or viral infection while increasing the reoccurance of autoimmunity and the incidence of malignancy/metastasis.Even though the molecular mechanisms by which chronic psychological stress inhibits the effector subsets in the immune system have been partially disclosed,it remains elusive whether chronic psychological stress also affects the regulatory subsets and if yes,what are the underlying mechanisms.Here,we review the findings in this field.
2.Research on the proliferative scars treated by skin regenerative method treatment
Xianzhong ZHAO ; Jiyan SUN ; Dongbo ZHANG ; Yongliang GE ; Dongjing YIN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(08):-
Objective: To observe the efficacy of skin regenerative medical technique in treating proliferative scars. Method: Select 32 patients (age16-52) with proliferative scars after burns or wound for 1-11 years,which include 25(male) and 7(female). 2 scar similar spots are chosen in each patient for self-comparison.After the experimental group uses the scar detachment, scar Pi Huizhi applies the beautiful valuable moist burn medicinal plaster gauze cover the cooperation of Chinese and Western medicine home position skin regenerative method treatment; After the control group uses the scar detachment, scar Pi Huizhi applies the petroleum jelly cover the traditional method treatment.The observation comparison curative effect, applies the Vancouver scar appraisal meter appraisal scar proliferation situation. Results: Two groups return to the scar skin which plants to survive.The experimental group regenerates the skin to be good, the cicatrization speed and the quality surpass the control group (P
3.A case report of pancreatic metastasis from prostate cancer
Zhengyu ZHOU ; Yimu ZHANG ; Yazhen HONG ; Jiyan BAI ; Dong YANG ; Pengcheng ZHAO ; Chaohong HE
Chinese Journal of Urology 2021;42(4):304-305
The most common metastatic site of prostate cancer is the bone, followed by the lung, bladder, liver, and adrenal gland. We report on a rare case of pancreatic metastasis from prostate cancer. A 52-year-old patient was admitted to the hospital with epigastric pain for 20 days. PET-CT showed malignant lesions in the prostate and pancreas, and prostate and pancreas puncture biopsies were performed, respectively. The patient was diagnosed as prostate cancer with pancreatic metastasis according to the pathological findings. After undergoing androgen deprivation therapy and docetaxel chemotherapy for 6 cycles, reexamination revealed that the pancreatic metastases had disappeared.
4.Clinical research of patients with acute or chronic hepatic failure treated with molecular adsorbent recirculating system.
XinMin ZHOU ; Xin WANG ; Yan YANG ; Lin ZHAO ; Jiyan MIAO ; Jie DING ; Daiming FAN
Chinese Journal of Hepatology 2002;10(3):213-215
OBJECTIVETo summarize the experience of a single treatment using molecular adsorbent recirculating system (MARS) in patients with acute-on-chronic liver failure.
METHODSTwenty-five eases treated by MARS-artificial liver were followed up and reviewed.
RESULTSThe levels of serum total bilirubin, non-conjugated bilirubin and blood ammonia were significantly decreased from (618.51 200.68) mmol/L to (390.81 146.02) mmol/L (t=2.729, P<0.01), (490.03 163.39) mmol/L to (303.28 113.06) mmol/L (t =2.516, P<0.01), and (152.44 82.62)mmol/L to (84.80 13.30)mmol/L (t=2.174, P<0.05), respectively. Prothrombin activity was significantly increased from 70.55% 32.39% to 93.63% 14.20% (t=1.728, P<0.05) in patients during a single 6 h treatment with MARS. No difference was presented in the markers of liver zymogram, serum protein, kidney function, electrolyte, blood routine and blood gas analysis before and after the MARS. Thirteen of 17 patients have been cured or improved, 4 died, and the survival rate was 76.5%.
CONCLUSIONSMARS is a safe and an effective treatment for patients with liver failure.
Adult ; Aged ; Ammonia ; blood ; Bilirubin ; blood ; Chronic Disease ; Female ; Follow-Up Studies ; Humans ; Kidney Function Tests ; Liver Failure, Acute ; blood ; therapy ; Liver Function Tests ; Liver, Artificial ; adverse effects ; Male ; Middle Aged
5. Efficacy and mechanism of Xiaobanxia Decoction and categorized formula in preventing and treating delayed chemotherapeutic vomiting
Jiyan ZHAO ; Jinxi LI ; Yimin ZHANG ; Zhenfei DONG ; Junning WANG
International Journal of Traditional Chinese Medicine 2020;42(2):138-143
Objective:
To observe the pharmacodynamic effect of
6.Expression, purification and refolding of extracellular domain of mouse epidermal growth factor receptor.
Bing HU ; Ling TIAN ; You LU ; Li YANG ; Xia ZHAO ; Jiyan LIU ; Yuquan WEI
Journal of Biomedical Engineering 2003;20(2):264-267
A DNA fragment encoding extracellular domain of mouse epidermal growth factor receptor (EGFR) was obtained by PCR from a previous recombinant plasmid. The DNA fragment was then ligated into prokaryotic expression vector, and expressed in Escherichia Coli. The recombinant protein was purified under denature conditions by affinity chromatography, and refolded with gradient dialysis. The recombinant protein could produce antibodies to recognize extracellular domain and full-length of mouse EGFR, and form homodimer in the presence of EGF detected by western blot analysis. These findings provide evidence that the renatured recombinant extracellular domain of mouse epidermal growth factor receptor is immunogenetic and may be important for further application of this protein in functional and immunological research.
Animals
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Escherichia coli
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genetics
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metabolism
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Genetic Vectors
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Mice
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Plasmids
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Receptor, Epidermal Growth Factor
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biosynthesis
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chemistry
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genetics
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isolation & purification
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Transfection
7. COVID-19 Pandemic: global epidemiological trends and China’s subsequent preparedness and responses
Yan GUO ; Yangmu HUANG ; Jie HUANG ; Yinzi JIN ; Wen JIANG ; Peilong LIU ; Fangjing LIU ; Junxiong MA ; Jiyan MA ; Yu WANG ; Zheng XIE ; Hui YIN ; Chunshan ZHAO ; Shuduo ZHOU ; Ji ZHANG ; Zhijie ZHENG ; Zhijie ZHENG
Chinese Journal of Epidemiology 2020;41(5):643-648
The outbreak of COVID-19 has spread quickly across 114 countries/territories/areas in six continents worldwide and has been announced as a pandemic by WHO. This study analyzed global COVID-19 epidemiological trends, examined impact of the pandemic on global health security, diplomacy, and social environment in China, and provided short- and long-term strategic policy recommendations for China’s subsequent preparedness and responses.
8.PRMT6 promotes tumorigenicity and cisplatin response of lung cancer through triggering 6PGD/ENO1 mediated cell metabolism.
Mingming SUN ; Leilei LI ; Yujia NIU ; Yingzhi WANG ; Qi YAN ; Fei XIE ; Yaya QIAO ; Jiaqi SONG ; Huanran SUN ; Zhen LI ; Sizhen LAI ; Hongkai CHANG ; Han ZHANG ; Jiyan WANG ; Chenxin YANG ; Huifang ZHAO ; Junzhen TAN ; Yanping LI ; Shuangping LIU ; Bin LU ; Min LIU ; Guangyao KONG ; Yujun ZHAO ; Chunze ZHANG ; Shu-Hai LIN ; Cheng LUO ; Shuai ZHANG ; Changliang SHAN
Acta Pharmaceutica Sinica B 2023;13(1):157-173
Metabolic reprogramming is a hallmark of cancer, including lung cancer. However, the exact underlying mechanism and therapeutic potential are largely unknown. Here we report that protein arginine methyltransferase 6 (PRMT6) is highly expressed in lung cancer and is required for cell metabolism, tumorigenicity, and cisplatin response of lung cancer. PRMT6 regulated the oxidative pentose phosphate pathway (PPP) flux and glycolysis pathway in human lung cancer by increasing the activity of 6-phospho-gluconate dehydrogenase (6PGD) and α-enolase (ENO1). Furthermore, PRMT6 methylated R324 of 6PGD to enhancing its activity; while methylation at R9 and R372 of ENO1 promotes formation of active ENO1 dimers and 2-phosphoglycerate (2-PG) binding to ENO1, respectively. Lastly, targeting PRMT6 blocked the oxidative PPP flux, glycolysis pathway, and tumor growth, as well as enhanced the anti-tumor effects of cisplatin in lung cancer. Together, this study demonstrates that PRMT6 acts as a post-translational modification (PTM) regulator of glucose metabolism, which leads to the pathogenesis of lung cancer. It was proven that the PRMT6-6PGD/ENO1 regulatory axis is an important determinant of carcinogenesis and may become a promising cancer therapeutic strategy.