1.p27 Loss Is Associated with Poor Prognosis in Gastroenteropancreatic Neuroendocrine Tumors.
Hee Sung KIM ; Hye Seung LEE ; Kyung Han NAM ; Jiwoon CHOI ; Woo Ho KIM
Cancer Research and Treatment 2014;46(4):383-392
PURPOSE: Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) represent a heterogeneous disease group originating from the neuroendocrine cells. Identification of prognostic markers, related to neuroendocrine tissue-selective tumorigenesis, is necessary to find therapeutic targets. MATERIALS AND METHODS: A total of 327 patients with GEP-NETs were included in this study; there were 49 gastric, 29 duodenal, 49 pancreatic, 12 hepatobiliary, 33 appendiceal, 5 proximal colon, and 150 distal colon cases. We performed immunostaining with the tissue microarray method for menin, p27, and p18. RESULTS: We observed negative staining for menin, p27, and p18 in 34%, 21%, and 56% of GEP-NETs, respectively. The loss of p27, but not menin, was positively correlated with the grade of Ki-67. Menin-/p27-, menin-/p27+, menin+/p27-, and menin+/p27+ phenotype groups included 13%, 22%, 8%, and 57% of patients, respectively. A dichotomized comparison showed that menin- or p27- tumors were significantly associated with foregut and midgut localizations, high World Health Organization (WHO) grade, lymph node metastasis, and more advanced stage as compared to menin+/p27+ patients. Kaplan-Meier analysis for the overall survival showed that p27 loss was significantly associated with decreased survival. Multivariate analysis showed that p27 loss is an independent factor for poor overall survival. CONCLUSION: Our results revealed that the loss of p27 is associated with poor prognosis and the menin-p27 pathway is important in the tumorigenesis of GEP-NETs.
Carcinogenesis
;
Colon
;
Cyclin-Dependent Kinase Inhibitor p27
;
Gastrointestinal Neoplasms
;
Humans
;
Kaplan-Meier Estimate
;
Lymph Nodes
;
Multivariate Analysis
;
Negative Staining
;
Neoplasm Metastasis
;
Neuroendocrine Cells
;
Neuroendocrine Tumors*
;
Pancreatic Neoplasms
;
Phenotype
;
Prognosis*
;
Biomarkers, Tumor
;
World Health Organization
2.Markers for Screening Lynch Syndrome Are Reliable and Useful for Identifying the Specimen Mislabeling.
Sun Ju BYEON ; Jiwoon CHOI ; Kyung Han NAM ; Bo Gun JANG ; Hee Eun LEE ; Min A KIM ; Woo Ho KIM
Korean Journal of Pathology 2012;46(2):131-136
BACKGROUND: During specimen processing in surgical pathology laboratories, specimen-related adverse events (SRAEs), such as mislabeling and specimen mixed-up might occur. In these situations, molecular techniques using short tandem repeat (STR) loci are required to identify the personal identity. Microsatellite instability (MSI) test is widely used for screening the hereditary non-polyposis colon cancer (Lynch syndrome) in surgical pathologies using polymorphic STR markers. We tried to evaluate the applicability of the MSI test for SRAEs. METHODS: We obtained 253 MSI test results to analyze the allele frequencies. After calibrating the estimated nucleotide lengths, we calculated the allele frequencies, a random match probability, and a likelihood ratio (LR) of three dinucleotide STR markers (D5S349, D17S250, and D2S123). RESULTS: The distribution of LR was 136.38 to 5,606,213.10. There was no case of LR<100. In addition, there were 153 cases (60.5%) of LR ranging from 100 to 10,000 and 100 cases (39.5%) of LR>10,000. Furthermore, the combined probability of identity was 9.23x10(-4) and the combined power of exclusion was 0.99908. CONCLUSIONS: Using the three STR markers that are recommended for MSI test, all the cases were positively identified in 1% range and about one-third cases showed high LR (>10,000). These results showed that MSI tests are useful to screen the personal identity in case of SRAE in pathology laboratories.
Biometric Identification
;
Colonic Neoplasms
;
Colorectal Neoplasms, Hereditary Nonpolyposis
;
Gene Frequency
;
Humans
;
Mass Screening
;
Microsatellite Instability
;
Microsatellite Repeats
;
Pathology, Surgical
;
Succinimides
3.Breast-feeding and Obesity in Early Childhood: Based on the KNHANES 2008 through 2011.
Miyong YON ; Haeng Shin LEE ; Dohee KIM ; Jeeyeon LEE ; Jiwoon NAM ; Gui Im MOON ; Jinhwan HONG ; Cho il KIM
Korean Journal of Community Nutrition 2013;18(6):644-651
Although there has been a significant increase in breast-feeding (BF) rate in Korea, it is plateaued since 2008 and still low compared with that of other countries. Because BF has been related to lower obesity prevalence in many studies and the increase in childhood obesity became evident in Korea, we wondered if a relatively lower BF rate has anything to do with this increase. Therefore, we looked into the relationship between mode & duration of BF during infancy and weight status of toddlers using the data from Korea National Health and Nutrition Examination Survey 2008 through 2011. Number of 2-3 year old toddlers with complete information on BF, anthropometry and normal birth weight was 674. While 87% of them were ever-breastfed, 6.2% each of them were either obese or overweight based on the Standard Growth Chart for Korean Children. Not only the obesity prevalence was different among groups of different mode of feeding, but also the mean duration of BF was significantly longer in normal weight group (9.2 mo.) compared with obese group (5.5 mo.). Accordingly, overweight and obesity prevalence of the toddlers breast-fed for 12 months or longer was significantly lower than that of the toddlers breast-fed for less than 12 months (OR 0.53, 95% CI 0.32-0.87). This study revealed that both BF and duration of BF affect the childhood obesity and, BF for 12 months or longer should be encouraged more aggressively as one of the main strategies to prevent and/or decrease childhood obesity in Korea.
Anthropometry
;
Birth Weight
;
Child
;
Growth Charts
;
Humans
;
Korea
;
Nutrition Surveys
;
Obesity*
;
Overweight
;
Pediatric Obesity
;
Prevalence
4.Dietary total sugar intake of Koreans: Based on the Korea National Health and Nutrition Examination Survey (KNHANES), 2008-2011.
Haeng Shin LEE ; Sung Ok KWON ; Miyong YON ; Dohee KIM ; Jee Yeon LEE ; Jiwoon NAM ; Seung Joo PARK ; Jee Young YEON ; Soon Kyu LEE ; Hye Young LEE ; Oh Sang KWON ; Cho Il KIM
Journal of Nutrition and Health 2014;47(4):268-276
PURPOSE: The aim of this study is to estimate total sugar intake and identify major food sources of total sugar intake in the diet of the Korean population. METHODS: Dietary intake data of 33,745 subjects aged one year and over from the KNHANES 2008-2011 were used in the analysis. Information on dietary intake was obtained by one day 24-hour recall method in KNHANES. A database for total sugar content of foods reported in the KNHANES was established using Release 25 of the U.S. Department of Agriculture National Nutrient Database for Standard Reference, a total sugar database from the Ministry of Food and Drug Safety, and information from nutrition labeling of processed foods. With this database, total sugar intake of each subject was estimated from dietary intake data using SAS. RESULTS: Mean total sugar intake of Koreans was 61.4 g/person/day, corresponding to 12.8% of total daily energy intake. More than half of this amount (35.0 g/day, 7.1% of daily energy intake) was from processed foods. The top five processed food sources of total sugar intake for Koreans were granulated sugar, carbonated beverages, coffee, breads, and fruit and vegetable drinks. Compared to other age groups, total sugar intake of adolescents and young adults was much higher (12 to 18 yrs, 69.6 g/day and 19 to 29 yrs, 68.4 g/day) with higher beverage intake that beverage-driven sugar amounted up to 25% of total sugar intake. CONCLUSION: This study revealed that more elaborated and customized measures are needed for control of sugar intake of different subpopulation groups, even though current total sugar intake of Koreans was within the range (10-20% of daily energy intake) recommended by Dietary Reference Intakes for Koreans. In addition, development of a more reliable database on total sugar and added sugar content of foods commonly consumed by Koreans is warranted.
Adolescent
;
Agriculture
;
Asian Continental Ancestry Group
;
Beverages
;
Bread
;
Carbonated Beverages
;
Coffee
;
Diet
;
Energy Intake
;
Food Labeling
;
Fruit
;
Humans
;
Korea
;
Nutrition Surveys*
;
Recommended Dietary Allowances
;
Vegetables
;
Young Adult