OBJECTIVE:To assess the preventing effect of nicardipine on stress reaction induced by direct laryngoscopy.ME_THODS:30 patients undergoing elective direct laryngoscopy were randomly divided into two groups:the routine group(group C)and nicardipine group(group N),15 patients for each group.In group C,anesthesia was performed with routine anesthetics,in group N,addition to routine anesthetics,nicardipine was administered by a bolus of 10?g/kg,there after 1～2?g/(kg?min)continuously intravenous administration.Blood pressure(BP),heart rate (HR) and blood sugar(Glu)were recorded perioperatively.RESULTS:The BP and HR increased in both groups from intubation to extubation,but when compared to the base value,the increase in group C had statistical significance (P0.05);There were significant differences between two groups at the same time points(P

Objective To explore occupational stress level and chronic illnesses prevalence of nurses in Xinjiang first-class hospital and provide scientific basis of reducing the nurses' occupational stress and improving health interventions.Methods Using stratified cluster sampling method,1 585 nurses were investigated occupational stress level and the chronic diseases who were from Xinjiang around the five states 3 armour hospital.Results Compared with national norm,the nurses had high professional task scored 177.79±29.85 and tension scored 102.46±22.18,individual coping scores from resources scored 119.84±24.06 were lower.The propotion of low,moderate,high tension nurse respectively was 6.1％ (97/1 585),47.1％ (746/1 585),46.8％ (742/1 585).Night shift frequency,nationality,title and average monthly income difference were statistically significant in terms of occupational stress level (x2=1.28-37.96,all P＜0.05).Joint disease,neck lumbar disease,chronic digestive system disease prevalence of nurse were higher,its morbidity rates were 10.9％(172/1 585),24.9％(394/1 585) and 22.1％(350/1 585) respectively.Presence of chronic diseases of the digestive system in high,medium and low tension was statistically difference (x2=9.558,P ＜ 0.01).Conclusions Occupational stress level of nurse is higher,and chronic diseases occurences are associated with occupational stress level.So the nurses occupational stress need to control so as to reduce chronic diseases.

At first,this paper introduces a kind of sickbed desk,which can be manipulated easily,and patients could live more comfortable with it.Secondly,it introduces universal lamp holder.Different light sources are used in different medical equipments and the connection of their bulbs and lamp holders are different,too.These lamp holders are expensive and apt to be damaged.In order to prolong the using time of the chilly light,and reduce the spoilage and cost,this paper designs an economic and durable universal lamp holder.

Objective To analyze the relationship between family factors and learning motivation of students in Xinjiang Medical University so as to provide scientific basis for promoting the medical undergraduates' learning motivation in the future.Method From April to June in 2014,using stratified cluster random sampling,2 000 college students from Xinjiang Medical University were surveyed by family factors and learning motivation questionnaire,and 1954 effective questionnaire were recycled.SPSS 17.0 was used to analyze the data,T test and variance analysis were conducted to the single factor and multiple linear regression analysis was made to multiple factors.Results All dimensions of 1 954 medical students' learning motivation shown were average,and the score of fear of failure was the lowest (2.82 ± 1.01).There were statistically significant differences in the scores of multiple dimensions of learning motivation of the medical students of different gender,nationality and grade (P＜0.05).And the scores in learning motivation in multiple dimensions of the medical students of different types of accounts,different family relationships,and different parental education level and whether they were the only child also had statistically significant difference (P＜0.05).Multi factor analysis showed that learning motivation,family account type,grade,gender,race,whether they were poor students and family relationships were the factors influencing the learning motivation of medical students.Conclusion Family factors have a great influence on the generation and maintenance of medical students' learning motivation,and it is necessary to change the poor education environment of the family,so as to interfere the students' learning motivation.

Objective:To investigate the feasibility of rectus muscle insertion measurement with anterior segment optical coherence tomography (AS-OCT) after corrective surgery of concomitant exotropia.Methods:A cross-sectional study was performed.Twenty-five eyes of 25 patients with concomitant exotropia were included in Shenyang Aier Eye Hospital from March 2018 to February 2019, and all patients received concomitant exotropic corrective surgery.The distance of limbus to rectus muscle insertion was measured before operation, at the beginning and at the end of operation with a caliper and at postoperative 2 weeks, 1 month, 3 months and 6 months with AS-OCT, respectively.Degree of strabismus was examined by the alternate prism cover test at 33 cm.The research followed the Declaration of Helsinki and the protocol was approved by a Medical Ethics Committee of Shenyang Aier Eye Hospital (No.2017KJB005). Written informed consent was obtained from the patients/custodians prior to any medical examination and treatment.Results:A total of 44 rectus muscles of 25 eyes with concomitant exotropia were measured, including 25 lateral rectus muscles and 19 medial rectus muscles.The detection rate of the muscle insertion by AS-OCT was low at 2 weeks and 1 month after surgery.The mean distance of limbus to lateral rectus muscle insertions was (12.30±1.40), (11.72±1.45) and (11.84±1.27)mm at the end of operation and postoperative 3 and 6 months, respectively, showing a significant difference among them (F=4.085, P=0.023), and the distance measured at the end of operation was significantly larger than that at postoperative 3 months (P=0.018). The mean distance of limbus tomedial rectus muscle insertion was (5.12±0.42), (5.56±0.61) and (5.28±0.57)mm at the end of operation, postoperative 3 and 6 months, respectively, and there was no significant difference among the three time points (F=3.912, P=0.055). The degree of strabismus of the patients at various time points after surgery were significantly reduced in comparison with that before surgery (all at P<0.05).Conclusions:AS-OCT can track the position of extraocular muscle insertions after concomitant exotropic corrective surgery.

Objective To generate high purity and maturity DC from human peripheral blood monocytes in vitro.Methods PBMC were isolated directly from whole blood by density gradient centrifugation and purified by collecting the attached cell,DC were then generated by induction and culturing PBMC for five days with RPMI1640 medium containing rhGM-CSF and rhIL-4 in vitro,and under the condition of 37 ℃,5% CO2.On the fifth day,rhTNF-α was added into DCs cultures,which were then incubated for three additional days.The morphology was monitored by light microscopy,and the phenotypes were determined by FCM.Results After eight days of culture,the cells developed typical and significant dendritic morphology and plenty of cells expressed CD1a, CD80 and CD83,features of DC.Including(78.07±9.43)%CD1a,(60.11±20.50)% CD80 and(46.82±14.15)% CD83 were expressed.About (3.12±1.30)x106 DC cells were derived from 40ml human peripheral blood.Conclusion The way to generate DCs is simple and easy.The DCs produced by this method acquired high purity and maturity antigenic characteristics of DCs.

Objective To search a kind of ideal seeded cell of bone tissue engineering. Methods Bone marrow tissue was fetched from rabbit,got bone marrow stromal cells by the method of density centrifuge,observed the conformation,growing feature and osteogeneric capability of the cells during culture in vitro by light microscope,transmission electron microscope and measure of osteogeneric capability. Results Most of the cultivated bone marrow stromal cells were triangle-like or shuttle-like cells. They growed and breeded rapidly,and had osteogeneric capability. They were easy to differentiate to osteoblasts. Conclusion The cultivated bone marrow stromal cells are matured ones,and could become the more ideal seeded cells for bone tissue engineering.

Objective To evaluate the clinical application of diffusion-weighted MR of low field in hypera-cute cerebral infarction. Methods Conventional MRI and DWI on 0.35T were performed in 15 patients with highly suspicious hyperacute infarction within 6 hours after presenting symptoms. The coincidence between conventional MRI ,DWI and final clinical diagnosis was analyzed to compare the sensitivity and specificity of diagnosis among DWI,ADC and conventional MRI. Results 12 patients were diagnosed as hyperacute cerebral infarction, identical with final clinical diagnosis. 3 patients were negative on DWI ,who were finally diagnosed as TIA by clinical doctors.The high-intensity zone on DWI appeared infarction in following CT or MRI examination. The sensitivity and speci-ficity of DWI were both 100% ,and those of conventional MRI was 20% and 100% ,respectively. The infarctional region on DWI appeared high intensity and it was in low intensity on ADC maps. Conclusion DWI combined with ADC maps can be an important index in the diagnosis of hyperacute cerebral infarction. Its accuracy for diagnosis was high, which provides objective proofs for early clinical thrombolysis treatment.

Objective To optimize the extraction technology of Taxus x media by using the contents of Paclitaxel and 10-deacetylbaccatin(10-DAB),two representative active diterpene alkaloids of taxane type from T.x media,as evaluation standard.Methods The smashing tissue extraction(STE)of Paclitaxel and 10-DAB from T.x media,was investigated by comparing with ultrasonic extraction(UE)which was one of the modern technologies of extraction.Results STE was more efficient than UE,and the contents of 10-DAI3 and Paclitaxel in the extracts obtained by STE were higher than those by UE.Conclusion STE is a fast,high-performance,and energy-saving technology for the extraction of diterpene alkaloids of taxane type.STE also provides a simple,component-safe,workable,and highly efficient method for the extraction of active natural product.

Objective To investigate the safety and feasibility of barbed absorbable suture line in retroperitoneoscopic partial nephrectomy. Methods 45 patients who were suitable for retroperitoneoscopic partial nephrectomy from February 2013 to February 2014 were collected and randomly divided into V-Loc group and the control group. 23 cases of V-Loc barbed absorbable suture line were chosen by suture kidney in V-Loc group.There were 22 cases of coated vicryl plus antibacterial suture and Hem-o-lok clip in the control group. The differences of operative time, blood loss, warm ischemia time, suture time, hospital stay and suture material costs were compared respectively between the two groups. Results All 45 cases of retroperitoneoscopic partial nephrectomy were successfully performed, without conversion to open surgery and important intraoperative complications. Postoperative pathology report showed that there were 37 cases with renal clear cell carcinoma, 3 cases with papillary renal cell carcinoma, 2 cases with chromophobe renal cell carcinoma with negative surgical margin, 3 cases with renal angiomyolipomas. In the V-Loc group and the control group, the average operative time were (90.8±9.6) and (96.9±9.1) min (P>0.05), the mean blood loss were (80.4±24.2) and (99.5±24.8) ml (P>0.05), the mean warm ischemia time were (21.5±3.4) and (25.3±1.9) min (P<0.05), suture time were (10.8±1.7) and (16.8±3.1) min (P<0.05), respectively, postoperative hospital stay were (5.7±1.7) and (7.1±1.9) d (P>0.05), the average cost of renal parenchymal suture material were (1 215.5±101.4) and (2 073.5±301.8) yuan (P<0.05). The warm ischemia time,suture time and suture material costs in the V-Loc group were significantly lower than those in the control group (all P<0.05). Conclusion The application of barbed absorbable suture line in retroperitoneoscopic partial nephrectomy can shorten warm ischemia time and suture time, and save hospitalization costs, with favorable safety and feasibility.