Objective To analyse the X-ray and CT manifestations of sacral primary tumor.Methods 10 cases with sacral primary tumors verified by pathology,including sacral chordoma(n=5),giant cell tumor(n=2),chondrosarcoma(n=2),neurilemmomas(n=1),were analysed retrospectively.Results (1)The manifestations of CT and X-ray of sacral chordoma presented as a midline destructive lesion,involving prominently at sacrum 3～5,with flocculent calcification and irregular bone crests.(2)Giant cell tumor involved predominately sacrum 1～3 with expansile destructive area and commonly in young adults.(3)Chondrosarcoma and neurilemmomas had difference characteristic on X-ray film and CT.One case with chondrosarcoma had bone destruction in sacrum 1～3 and giant mass grow into pelvis.Conclusion CT combined with X-ray film is helpful in diagnosis and differential diagnosis of sacral primary tumors.

Objective To establish an assay for the detection of Streptococcus pneumoniae by real-time fluorescence quantititive polymerase chain reaction (PCR).Methods Special primers and probe for the autolysin A (lytA)gene were designed.The sensitivity and specificity of primers and probe were studied,and cut-off of cycle threshold was assayed.158 clinical specimens were confirmed by real-time fluorescence quantitative PCR and bacterial culture method.Results Primer and probe design for LytA gene could sensitively detect serotype Streptococcus pneumoniae strains of common pathogenic,and the sensitivity was 100 copies.Among 35 strains of Streptococcus pneumoniae,34 cases were detected to be positive for Streptococcus pneumoniae by real-time fluorescence quantitative PCR,while 1 case was detected to be negative;among 15 strains of non-Streptococcus pneumoniae, all were detected to be negative.Among the 158 clinical sputum specimens,34 cases with Streptococcus pneumoniae were detected by real-time fluorescence quantitative PCR,while only 10 cases with Streptococcus pneumoniae were detected by the culture method.White blood cells count and time in hospital of cases with Streptococcus pneumoniae were higher than those of cases without Streptococcus pneumoniae (P <0.05 ). Conclusion Real-time fluorescence quantitative PCR is a sensitive and specific assay for the detection of Streptococcus pneumoniae.It can be used for the diagnosis of Streptococcus pneumoniae.

Objective:To test the validity and reliability of the Perceived Stress Scale-14 items (PSS-14) applied in cancer survivors.Methods:Totally 652 cancer survivors (patients who had finished active treatments and were in convalescence) were selected and a field questionnaire survey was conducted among them.Exploratory factor analysis and confirmatory factor analysis were performed to test the construct validity of PSS-14.Correlation between the two dimensions (discriminant validity) and correlation between stress and quality of life were analyzed to further test its validity.The intemal consistency reliability,split-half reliability,retest reliability and sensitivity analysis were adopted to test the reliability of PSS-14.Results:Two common factors were extracted in the exploratory factor analysis.The cumulative percentage of variance explained was 60.8％.The index of confirmatory factor analysis were:TLI =0.92,CFI =0.93,SRMR =0.07,RMSEA =0.08.The correlation coefficient between the two dimensions of PSS-14 was-0.16 (P ＜0.001).The correlation coefficients between the score of PSS-14 and each dimension of EORTC QLQ-C30 were 0.24-0.55 (P ＜ 0.001).The Cronbach α coefficients of the whole scale and the two dimensions named "perceived coping ability" and "perceived distress" were 0.78,0.91 and 0.88 respectively.After the scale was split into two parts,the Cronbach α coefficients of each part were 0.66 and 0.60.The Spearman-Brown coefficient was 0.79.The Guttman split-half coefficient was 0.79.After one item was removed from the scale each time,the Cronbach a coefficients of the remaining items varied from 0.75 to 0.77.The retest reliability of the whole scale and the two dimensions were 0.89,0.86 and 0.85 respectively.Conclusion:PSS-14 shows favorable validity and reliability,suggesting the utility to assess stress of cancer survivors as a self-administered inventory.

Objective:To understand the work and training status of multi-region medical nursing assistants, and provide reference for standardizing the admission qualification of medical nursing assistants and improving the training system of them.Methods:Using the purposive sampling method, in-depth interviews were conducted among 19 medical nursing assistant managers and 20 medical nursing assistants from 13 large general hospitals in China from October to November 2021. Colaizzi 7-step analysis method was used to analyze data and extract themes.Results:Two themes of status of training of medical nursing assistants and managers' expectations for the development of the medical nursing assistants industry were extracted. It included 7 sub-topics, including insufficient pre-job training hours and relative single content, low on-the-job training coverage, the need to improve the practicality of training, urgent improvement of assessment system, standardization of admission qualifications for medical nursing assistants, establishment of training structure for medical nursing assistants and implementation of graded nursing care for medical nursing staff.Conclusions:The admission qualification of medical nurses should be strictly regulated, the government should take the lead in formulating standardized and professional pre-job training programs, carry out hierarchical, focused and multi-form on-the-job training, build a training and assessment system including "process evaluation" and "final evaluation", and implement hierarchical employment to stabilize the team of medical nursing assistants.

BACKGROUND: Hemolysis is an important problem in the design and development of artificial heart pumps. Hemolysis is associated with the shear force of blood cells in the pump and the movement time in the pump. OBJECTIVE: To analyze the relationship between the operating parameters of blood pump (flow rate of blood pump), the structure of blood pump (outlet width of blade) and the shear stress of blood cells in artificial heart pump. METHODS: The basic structure of the centrifugal blood pump was designed according to the velocity coefficient method. The three-dimensional model of the blood pump, mesh division and flow field simulation were established by using CFturbo, ANSYS ICEM and FLUENT software respectively to study the best mesh division method. The results of the numerical simulation corrected the diameter of the impeller outlet to finally obtain an artificial heart pump meeting the needs of the human heart. In order to reduce the shear stress and movement time of blood cells in the heart pump, the optimal design and analysis of the impeller inlet flow rate Q (2, 3, 4, 5, 6, 7 L/min) and the operating parameter blade outlet width b2 (2.0, 2.1, 2.2, 2.3, 2.4, 2.5 mm) of the centrifugal artificial heart pump were designed to reduce the probability of hemolysis. RESULTS AND CONCLUSION: (1) As the flow rate increased, the shear stress of the blood cells in the blood pump gradually increased, and the movement time gradually decreased. As the flow rate increased, the standard hemolysis value in the blood pump gradually decreased, and the damage to the blood cells in the blood pump alleviated. When the flow rate was 7 L/min, the standard hemolysis value in the blood pump was the lowest. (2) With the increase of blade outlet width, the shear stress of the blood in the pump decreased, and the movement time of the blood in the pump increased. When the blade outlet width was 2.0-2.5 mm, the hemolysis value in the blood pump decreased with the increase of the blade outlet width. When the blade outlet width was about 2.5 mm, the hemolysis value in the blood pump was the lowest.

Objective:Through TTC staining, immunohistochemical analysis, RT-PCR and hind limb motor function evaluation and other experimental methods, to explore the regulatory mechanism of metformin on anti-apoptosis in rats with spinal cord injury (SCI).Methods:Establish a rat spinal cord injury model. Through Basso-Beattie -Bresnahan locomotor rating scale (BBB) and cant test to evaluate the recovery of hindlimb motor function in rats. The changes of necrotic area of spinal cord tissue were compared by TTC staining. Extraction of rat spinal cord tissue, by Dot blot analysis and immunohistochemical detection of the hydroxyl of DNA methylation level. By qPCR, Western Blot detection TET2mRNA and protein expression level, and the changes in the scope of spinal cord injury were detected by inhibiting the expression of TET2. The interaction between TET2 and Foxo3a was detected by immunoblotting and immunoprecipitation. Through RT-PCR assay Foxo3a downstream related changes in the level of gene expression.Results:Compared with the SCI+NS group, the necrotic area of the spinal cord tissue was reduced after metformin treatment, and the BBB score and the incline test score were higher ( P<0.05). At the same time, we found that the levels of TET2mRNA and protein increased significantly after SCI at 24 h, and the 5-hmC level of DNA increased. The levels of TET2mRNA and protein and 5-hmC increased further after the use of metformin. After using SC-1, compared with the SCI+MET group, the level of 5-hmC decreased and the area of infarction increased. After SCI, the mRNA levels of downstream genes Bim, P27kip, Bax increased significantly. After metformin treatment, the mRNA levels of Bim and Bax were lower than those in the SCI+NS group ( P<0.05). After SCI, the 5-hmC levels of downstream genes Bim, P27kip, Bax increased significantly. After metformin treatment, the 5-hmC levels of Bim and Bax were lower than those in the SCI+NS group ( P<0.05). Conclusion:Metformin can promote the interaction between TET2 and Foxo3a, increase the 5-hmC level of the overall DNA, and inhibit the activation of related apoptosis genes, thereby improving tissue damage and nerve function recovery after spinal cord injury.

Objective To explore the expressions Bmi-1 and P16 and their significances of in patients with breast cancer and to analyze their relationships with biomarkers,such as ER,PR and HER-2.Methods The expressions of Bmi-1 and P16 were detected by immunohistochemical SP method in 80 patients with breast cancer and their relationships were evaluated.Results The expres-sion of Bmi-1 was positively related with the TNMstaging,histological degrees and lymph node metas-tasis (P <0.05),but had no association with age,tumor volume as well as the expressions of ER,PR and HER-2 (P >0.05).Compared with normal tissues,the low expression of P16 was in reverse rela-tionship with the TNMstaging,histological degrees and lymph node metastasis (P <0.05),related with HER-2 expression (P <0.05),but had no association with age,tumor volume as well as the ex-pressions of ER and PR (P >0.05).Conclusion The expressions of Bmi-1 and P16 proteins play an important role in the recurrence and metastasis of breast cancer,and their combined detection can be used as an important indicator for the prognosis of breast cancer.

Objective To explore the expressions Bmi-1 and P16 and their significances of in patients with breast cancer and to analyze their relationships with biomarkers,such as ER,PR and HER-2.Methods The expressions of Bmi-1 and P16 were detected by immunohistochemical SP method in 80 patients with breast cancer and their relationships were evaluated.Results The expres-sion of Bmi-1 was positively related with the TNMstaging,histological degrees and lymph node metas-tasis (P <0.05),but had no association with age,tumor volume as well as the expressions of ER,PR and HER-2 (P >0.05).Compared with normal tissues,the low expression of P16 was in reverse rela-tionship with the TNMstaging,histological degrees and lymph node metastasis (P <0.05),related with HER-2 expression (P <0.05),but had no association with age,tumor volume as well as the ex-pressions of ER and PR (P >0.05).Conclusion The expressions of Bmi-1 and P16 proteins play an important role in the recurrence and metastasis of breast cancer,and their combined detection can be used as an important indicator for the prognosis of breast cancer.

Objective:To construct an unaccompanied patient ward management system based on the harmony theory and explore its effects in the Department of Breast and Plastic Surgery.Methods:Patients from the Department of Breast and Plastic Surgery, the University of Hong Kong-Shenzhen Hospital from January to July 2021 were selected as the control group ( n=1 213) , who received conventional accompanied care. Those admitted from January to July 2022 were the observation group ( n=1 057) , who received harmonious unaccompanied care. The occurrence rate of adverse events, patient satisfaction, and medical staff's job satisfaction between the two groups were compared. Results:The occurrence rate of adverse events in the observation group (0/1 057) was lower than that in the control group (1/1 213) , with no statistically significant difference ( P>0.05) . Both patients' satisfaction and medical staff's job satisfaction in the observation group were higher than those in the control group, with statistically significant differences ( P<0.05) . Conclusions:The construction of the unaccompanied patient ward management system based on the harmony theory contributes to standardizing high-quality nursing comprehensively. It improves both patients' satisfaction and medical staff's job satisfaction, promoting harmonious doctor-patient relationships.

MicroRNAs (miRNAs) that exert function by posttranscriptional suppression have recently brought insight in our understanding of the role of non-protein-coding RNAs in carcinogenesis and metastasis. In this study, we described the function and molecular mechanism of miR-139-5p in colorectal cancer (CRC) and its potential clinical application in CRC. We found that miR-139-5p was significantly downregulated in 73.8% CRC samples compared with adjacent noncancerous tissues (NCTs), and decreased miR-139-5p was associated with poor prognosis. Functional analyses demonstrated that ectopic expression of miR-139-5p suppressed CRC cell migration and invasion in vitro and metastasis in vivo. Mechanistic investigations revealed that miR-139-5p suppress CRC cell invasion and metastasis by targeting AMFR and NOTCH1. Knockdown of the two genes phenocopied the inhibitory effect of miR-139-5p on CRC metastasis. Furthermore, the protein levels of the two genes were upregulated in CRC samples compared with NCTs, and inversely correlated with the miR-139-5p expression. Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. Together, our data indicate that miR-139-5p is a potential tumor suppressor and prognostic factor for CRC, and targeting miR-139-5p may repress the metastasis of CRC and improve survival.
Animals ; Base Sequence ; Cell Line, Tumor ; Cell Movement ; genetics ; Colorectal Neoplasms ; genetics ; pathology ; therapy ; Down-Regulation ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; HCT116 Cells ; HEK293 Cells ; Humans ; Male ; Mice, Inbred BALB C ; Mice, Nude ; MicroRNAs ; genetics ; Middle Aged ; Neoplasm Invasiveness ; RNA Interference ; Receptor, Notch1 ; genetics ; metabolism ; Receptors, Autocrine Motility Factor ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Nucleic Acid ; Survival Analysis ; Xenograft Model Antitumor Assays