Objective:Through a study on knowledge, attitudes, and practice of tobacco control of medical staff in Gansu Province, the paper aims to analyze the issues of participation, community tobacco control leadership and other aspects that impact the creation of a smoke-free healthcare system in order to provide a basis for the formulation of policies to improve the health professional mechanism of tobacco control. Methods:The stratified cluster sampling method was adopted to select 34 companies, accounting for a total of 805 medical staff as the study population in all kinds of health institutions. A self-designed questionnaire was adopted to investigate their knowledge, attitudes, and practice via on-site self-administered surveys. Results:the medical staff lacks knowledge as to the depth and breadth of damage from the use of tobacco. Although their attitude towards tobacco control is positive, the comprehensive to-bacco control service capabilities are insufficient. Logistic regression analysis showed that tobacco control capabilities of medical staff is related to their attitude toward smoking and whether or not they discourage smoking or asking about smoking . Conclusion:The key to the creation of a smoke-free health system to lead the community in tobacco control is the targeted tobacco cessation knowledge and skills training of medical staff to reduce smoking rates among medical staff and enhance the awareness of administrative staff.

Objective To investigate the relationship between social network behaviors and personality.Methods Both questionnaires including self-reported Renren questionnaire and the simplified Chinese version of NEO five-factor inventory (NEO-FFI) were completed in university students (n =509).Results (1) Individuals with higher score of Extraversion used Renren applications more frequently,such as using Wall (2.26 ± 0.51,2.10±0.40,t=3.163,P=0.002),Status Update (2.58 ±0.71,2.33 ±0.62,t =3.268,P=0.001),Image (2.05±0.88,1.81 ±0.72,t=2.628,P=0.009),and Photo (2.17 ±0.49,1.96 ±0.33,t=4.409,P＜0.01).(2)Individuals with higher score of Openness of Experience were more likely to use Gift (2.01 ± 0.49,1.83 ± 0.59,t =2.870,P =0.005).(3) Individuals who were willing to use Blacklist (41.93 ± 4.41,43.07 ± 4.72,t =2.207,P =0.028) and who were willing to send private messages (42.23 ± 4.79,43.27 ± 4.55,t =2.476,P =0.014) have higher score of Agreeableness.(4) Individuals who checked messages more often had higher score of Conscientiousness (41.59 ± 6.33,40.72 ± 5.67,37.56 ± 8.33,F =7.170,P =0.001).(5) Individuals with higher score of Neuroticism were more likely to be dependent to Renren (2.91 ± 1.01,2.48 ± 1.02,t =3.815,P ＜0.01).Individuals who were more likely to express their emotions on SNS had higher score of Neuroticism (33.10 ± 6.78,32.19 ± 6.16,31.36 ± 5.77,F=3.594,P =0.010).Conclusion The data suggest that the usage of social networking sites are related to personality factors.Personality can be used to predict university students' social networking behaviors.

Objective To investigate the relationship between personality and social network profile-based information.Methods 438 college students were recruited,and Big five inventory NEO-FFI was applied to all subjects.The participants' Renren web profile information were recorded.Results ①Individuals with higher score of Extraversion had more friends (2.47 ± 0.29,2.34 ± 0.27,F =17.861,P =0.000),shared more (2.51 ± 0.56,2.32 ± 0.57,F =8.617,P =0.004),pasted more photographs (2.30 ± 0.51,2.14 ± 0.56,F =5.523,P =0.02).② There was a U shape correlation (t =2.258,P =0.024,t =2.115,P =0.035) between levels of neuroticism and numbers of blogs (0.66 ± 0.71) and photographs (1.92 ± 0.83).③There was no difference on numbers of friends (2.38 ± 0.31,2.39 ± 0.27,F =0.149,P =0.700) and e-gifts received (1.30 ± 0.52,1.26 ± 0.45,F =0.257,P=0.613) between participants of high and low levels of agreeableness.④ Individuals with higher scores in openness shared more with others(2.46 ± 0.57,2.29 ± 0.58,F =5.248,P =0.023).⑤ Individuals being more conscientious had more blogs(1.08 ±0.49,0.96 ±0.48,F=3.064,P=0.082).Conclusion There are significant differences about profile-based information among college students with different personality.

In order to study the molecular mechanism and physiological significance of the interaction between PGRN and Rev-erbβ, the PGRN gene in HEK293 (Rev-erbβ-/-) marked as C3-6 cell lines was knocked out by CRISPR/Cas9 system to generate the Rev-erbβ and PGRN double genes knockout HEK293 cell lines. First, four sgRNAs were designed for PGRN gene, and PGRN sgRNA2 and sgRNA3 with the higher activity were used to construct the Lentiviral vector, pLenti/CMV-Loxp-Cas9-sgRNA2-U6-sgRNA3-U6-Loxp-EF1α-Puro. Then, the lentivirus vector carrying Cas9 and double PGRN sgRNA were used to infect HEK293 C3-6 cells. Through drug screening, cloning and sequencing, we obtained the monoclonal HEK293 (Rev-erbβ-/-; PGRN-/-) marked as C3-6/23 cell lines. Using qRT-PCR and Western blotting, we detected PGRN mRNA and protein expression in C3-6/23 cell lines. Finally, genetic complementation was used to study the effect of PGRN-mediated Rev-erbβ on the regulation of the target gene promoter transcriptional activity in the C3-6/23 cell lines. In HEK293 C3-6/23 cell lines, the two DNA chains of PGRN gene were both deletion mutagenesis, and the expression mRNA and protein of PGRN did not reach the detection level. At the same time, the interaction between PGRN and Rev-erbβ enhanced the regulation of Rev-erbβ on the transcription of target gene promoter in the cell lines. Using CRISPR/Cas9 system, we successfully constructed the double knockout HEK293 (Rev-erbβ-/-; PGRN-/-) monoclonal cell lines. The study found that PGRN could affect Rev-erbβ on the regulation of target gene promoter transcription in the C3-6/23 cell lines; however, the mechanism of PGRN involvement in mediating Rev-erbβ in transcriptional regulation remains to be further studied.

Since December 2019, a novel coronavirus (2019-nCoV, SARS-CoV-2) pneumonia (COVID-19) outbreak has occurred in Wuhan, Hubei Province, and the epidemic situation has continued to spread. Such cases have also been found in other parts of the country. The spread of the novel coronavirus pneumonia epidemic has brought great challenges to the clinical practice of thoracic surgery. Outpatient clinics need to strengthen the differential diagnosis of ground glass opacity and pulmonary plaque shadows. During the epidemic, surgical indications are strictly controlled, and selective surgery is postponed. Patients planning to undergo a limited period of surgery should be quarantined for 2 weeks and have a nucleic acid test when necessary before surgery. For patients who are planning to undergo emergency surgery, nucleic acid testing should be carried out before surgery, and three-level protection should be performed during surgery. Patients who are planning to undergo emergency surgery in the epidemic area should be confirmed with or without novel coronavirus pneumonia before operation, and perform nucleic acid test if necessary. Surgical disinfection and isolation measures should be strictly carried out. Among postoperative patients, cases with new coronavirus infection were actively investigated. For the rescue of patients with novel coronavirus infection, attention needs to be paid to prevention and treatment and related complications, including mechanical ventilation-related pneumothorax or mediastinal emphysema, and injury after tracheal intubation.