OBJECTIVETo establish the normal MESH diagrams of Chinese in Beijing, and to build a computerized MESH analysis system for orthodontic practice.

METHODSTwenty-eight subjects with normal occlusion were selected in Beijing and their lateral cephalograms were taken at the age of thirteen and eighteen, respectively. Individual MESH diagrams were then established for each subject mainly according to Moorrees' method from the cephalograms orientated in estimated natural head position. Male and female normal MESH diagrams were created. A computerized MESH analysis system was also developed.

RESULTSThe normal MESH diagrams of Chinese in Beijing, thirteen and eighteen years old respective, were established. The computerized MESH analysis system was constructed and used in orthodontic patients.

CONCLUSIONSMESH analysis is a proportional analysis method. It can show the results directly, succinctly and holistically. It is also a favorable complement and amendment to the commonly used angle and linear X-ray analysis methods.

Adolescent ; Asian Continental Ancestry Group ; Cephalometry ; methods ; Dental Occlusion ; Female ; Humans ; Image Processing, Computer-Assisted ; methods ; Male ; Radiography ; Skull ; anatomy & histology ; diagnostic imaging

OBJECTIVETo observe the effect of Wenyang Huazhuo Tongluo Recipe (WYHZTLR) on the proportion of T helper 17 cells (Thl7)/regulatory T cells (Treg), and serum levels of IL-17 and IL-10 in peripheral blood of systemic sclerosis (SSc) patients with yang qi insufficiency and turbidity induced collaterals blockage syndrome (YQITICBS).

METHODSTotally 82 SSc patients were randomly assigned to the Western medicine group (as the control group) and the integrated Chinese and Western medicine group (as the treatment group), 41 cases in each group. All patients took methotrexate (MTX) tablet and prednisone tablet. Patients in the treatment group additionally took WYHZTLR. The treatment course for all was six consecutive months. Besides, another 70 healthy volunteers were recruited as a healthy control group (as the healthy group). Percentages of Th17 and Treg in peripheral blood were detected by flow cytometry. Serum levels of IL-17, IL-10, von Willebrand factor (vWF), aminoterminal propeptide of type l procollagen (PIIINP), and cross-linked carboxyterminal telopeptide of type I collagen ( I CTP) were measured by double antibody sandwich ELISA. The correlations between Th17/Treg and levels of vWF, PIIINP, I CTP, skin score, and disease activity index were observed by Pearson correlation analysis.

RESULTSThe percentage of Th17 in peripheral blood, ratios of Th17/Treg, and the serum level of IL-17 were significantly higher, but the percentage of Treg and the serum level of IL-10 were significantly lower in SSc patients, when compared with those of the healthy group (P <0. 05, P <0. 01). Compared with the same group before treatment, the percentage of Thl7, ratios of Thl7/Treg, and levels of IL-17, vWF, and PIIINP all decreased in the two groups after treatment (P <0.05, P <0.01), but the percentage of Treg and the IL-10 level increased (P <0. 05, P <0. 01). Meanwhile,the level of I CTP was higher in the treatment group after treatment (P <0. 05). The improvement of all indices except the percentage of Th17 was more obvious in the treatment group than in the control group (P <0. 05, P <0. 01). The ratio of Th17/Treg was positively correlated with levels of vWF, PIIINP, skin score, and disease activity index before and after treatment respectively (P <0. 01), but with no obvious correlation with the level of I CTP (P >0. 05).

CONCLUSIONWYHZTLR could achieve its therapeutic effect on SSc patients by regulating Th17/Treg imbalance, lowering levels of vWF and PIIINP, and elevating the level of I CTP.

Drugs, Chinese Herbal ; therapeutic use ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Humans ; Interleukin-10 ; Interleukin-17 ; Methotrexate ; Peptide Fragments ; Procollagen ; Scleroderma, Systemic ; drug therapy ; T-Lymphocytes, Regulatory ; Th17 Cells

OBJECTIVETo analyze the morphological characters of Angle's Class III malocclusion patients and the relationship between characters and treatment options.

METHODSA total of 472 Class III patients were collected during 1997-2000. The standardized coordinates' values of the patients were obtained by Procrustes superimposition (PS). Cluster analysis was carried out to divide the patients into different subdivisions and the relationship between treatment options and subdivisions was analyzed.

RESULTS472 patients were divided into 14 subdivisions with special characters. The morphological templates were formed by computer. There were certain relationships between characters and treatment options.

CONCLUSIONThe mechanism of malocclusion of different Class III patients are not necessarily same. The typing has significance to choose the treatment method.

Female ; Humans ; Male ; Malocclusion ; Malocclusion, Angle Class III ; Orthodontic Appliances

OBJECTIVETo explore Chinese medical syndrome distribution features of Japanese encephalitis (JE), and to analyze its correlation between syndromes and features of etiologies and pathogeneses.

METHODSRecruited were 277 patients with confirmative diagnosis of JE from Wuhan Medical Treatment Center, Children's Hospital Affiliated to Chongqing Medical University, Fifth People's Hospital of Guiyang City, Hangzhou Sixth People's Hospital, and Chengdu Hospital of Infectious Diseases between July to September 2012. Chinese medical syndrome distribution features were summarized from their general materials and detailed records of clinical data, including medical history, symptoms and signs, tongue fur, and pulse figures.The frequency of symptoms and signs was calculated according to mild, ordinary, severe, extreme severe degrees. The distribution of Chinese medical syndromes was summarized. And its correlation between syndromes and features of etiologies and pathogeneses were analyzed.

RESULTSAfter clustering analysis, Chinese medical syndromes of JE could be categorized as four groups: toxicity accumulation in Fei and Wei syndrome (TAFWS), brain collateral impaired by poison syndrome (BCIPS), depression of toxicity in the pericardium syndrome (DTPS), exhaustion of yin and yang syndrome (EYYS). BCIPS and DTPS were dominated, accounting for 74.0% (205 cases). The main causes covered evil of summer heat [accounting for 92.42% (256/277 cases)], heat [accounting for 87.73% (243/277 cases)], and toxin [accounting for 99.64% (276/277 cases)].

CONCLUSIONSThe four Chinese medical syndrome types of JE met Chinese medical clinical features of encephalitis. It is induced by infestation of dampness-heat, resulting in toxicity accumulation in Fei and Wei, brain collateral impaired by poison, depression of toxicity in the pericardium. Yin fluid and blood is exhausted as time goes by. Qi and yin are impaired to form intermingled deficiency and excess, and finally causing exhaustion of yin and yang.

Adolescent ; Child ; Child, Preschool ; Encephalitis, Japanese ; diagnosis ; pathology ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Medicine, Chinese Traditional ; methods ; Yang Deficiency ; diagnosis ; Yin Deficiency ; diagnosis

To assay the influence of dendritic cells(DCs)on the function of anti-infective immunity to Penicillium marneffei.DCs were generated from peripheral blood mononuclear cells(PBMC)and pulsed with Penicillium marneffei yeasts.DCs morphology was observed by the inverted microscope and cell surface markers of DCs were analyzed by flow cytometry.The concentrations of IL-12p70 were detected by ELISA. Mixed lymphocyte reaction was performed to assay the proliferation of T cells.The mRNA of CCR7 and CXCR4 were detected by the Real-time PCR quantifications.The acquired DCs exhibited irregular appearance and numerous long dendrites under light microscope.DCs and Penicillium marneffei yeasts were co-cultured for 24 h,numerous yeasts were observed inside the cells;an enhanced expression of the cell sur-face markers CD86、CD83、HLA-DR and CD40 were demonstrated;the expression of CCR7 and CXCR4 mRNA were also increased;the improved proliferation of T cells were observed in the mixed lymphocyte reaction.Yeasts-pulsed DCs secreted more IL-12p70 than that of non-pulsed,but less than that of LPS-pulsed DCs.DCs can engulf the Penicillium marneffei yeasts.When pulsed with Penicillium marneffei yeasts,DCs improved their expression of the co-stimulatory molecules and chemokine receptor CCR7、CXCR4,enhanced their capacity to process antigen.DCs play an important role in host defense against Penicillium marneffei infection.But the low level of the IL-12p70 production may lead to deficiency in the cell-mediated immunity against Penicillium marneffei.

OBJECTIVETo investigate the changes of available spaces of posterior arch segments in mandibular dentition from 13 to 18 years of age, which would provide information for space analysis of whole dental arch in adolescents.

METHODSTwenty-six adolescents (12 males, 14 females) with normal occlusion were selected. All of the subjects had lateral cephalograms taken at the same time each year for 6 years. Longitudinal cephalometry and statistic analysis were conducted for all subjects.

RESULTSSignificant differences of available space were found between males and females. The growth of available space from 13 to 18 years of age was 5.12 mm in females and 5.79 mm in males. The completion of mandibular remodeling in males lagged behind females for about 1 year. No significant increase was found in girls beyond 16 years and in boys beyond 17 years. There was a 1.22 mm increase on each side per year in females until 16 years and a 1.45 mm increase in males until 17 years. The increase of available space was made partly by resorption of bone from the anterior border of the ramus and partly by mesial movement of the first molar. The mesial movement of dental arch was probably stimulated by the eruption of the third molars.

CONCLUSIONSThe prediction of available space should be based on age and sex.

Adolescent ; Age Factors ; Cephalometry ; Dental Arch ; growth & development ; Female ; Humans ; Longitudinal Studies ; Male ; Mandible ; growth & development ; Molar, Third ; growth & development ; Orthodontics, Corrective ; Sex Factors ; Tooth Eruption ; Tooth Migration

Objective To evaluate the craniofacial and occlusional changes of Class Ⅱ patients treated with Tip-Edge Plus technique.Methods Twelve Class Ⅱ patients(7 girls and 5 boys,mean age 14.3 years)with bimaxillary or maxillary protrusion were selected.All the patients were four first premolar extraction cases and treated with Tip-Edge Plus technique.Cephalometric analysis Was performed to evaluate the changes before and after treatment.Results After treatment,the profile Was greally improved.UL-E,LL-E were decreased significantly(P<0.01)and incisors were retracted(P<0.05).Upper molars noved forward slightly(P>0.05).Conclusions The anchorage control in Tip-Edge Plus technique was effective without extraoral force,palatal arch and implants.Under very light force,the crown of anterior teeth tipped distally and lingually quickly.In the final stage,it was possible to achieve both desired root uprighting and crown torque.

Objective To investigate the craniofacial and occlusal changes of Class Ⅲ cases treated with Tip-Edge Plus Technique.Methods Thirteen Class Ⅲ patients(6 girls and 7 boys,mean age 14.9 years)with mandibular protrusion were selected.All the patients were four premolar extraction cases and treated with Tip-Edge Plus technique.Cephalometric analysis was performed to evaluate the changes before and after treatment.Results After treatment,Wits changed from(-2.50±1.40)mm to (-1.00±0.85)mm and ANB changed from(-0.61±1.41)°to(1.31±1.66)°(P＜0.05).UL-E and LL-E were decreased significantly from(7.55±1.24)mm to(4.82±1.05)mm and from(10.16±2.43)mm to(6.03±2.01)mm respectively(P＜0.01).The incisors were retracted.A remarkable soft tissue change was noted after the treatment.The difiefence between the distance of the upper lip and lower lip to SnPg'at the beginning of treatment changed from(-1.57±1.24)mm to(0.62±1.24)mm (P＜0.01).FCA changed from(2.62±1.53)°to(7.02±2.21)°.Conclusions With Tip-Edge Plus straight-wire technique and light continuous force,anterior teeth could be tipped distally and lingually quickly.The hard and soft tissues changed accordingly.

OBJECTIVETo explore the molecular mechanisms of apoptotic NB4 cells induced by the histone deacetylase inhibitor, sodium butyrate(SB).

METHODSSB was exposed to NB4 cells at a final concentration of 1.0 mmol/L. The untreated and treated cells were analysed with FACS and 2-dimensional electrophoresis (2-DE) at 0, 12, 24, 48 and 72 h. The changed protein spots were identified with MALDI-TOF-MS and ESI-TOF-MS/MS.

RESULTSSB induced apoptosis of NB4 cells. Twenty-one changed proteins involving apoptotic signal transduction, immunological regulation, transcriptional control, cellular metabolism, molecular transport and so on were identified. Thirteen of them had been reported to be related to apoptosis.

CONCLUSIONSB can induce apoptosis and many functional protein changes in tumor cells. These results pave the way to further explore the anti-tumor mechanism of SB.

Apoptosis ; drug effects ; genetics ; Butyrates ; pharmacology ; Cell Line, Tumor ; Flow Cytometry ; Humans ; Proteomics

This study was aimed to analyze the different proteomes between human acute leukemia (AL) cells and normal white blood cells by proteomic technology in order to lay the basis for diagnosing AL and understanding the mechanism of leukemogenesis. The proteins from AL cells of 40 AL patients identified by FAB classification and proteins from normal lymphocytes and granulocytes of 20 normal volunteers were separated by two-dimensional electrophoresis (2-DE), and the differentially expressed proteins between the two groups were identified by both matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electronspray ionization (ESI)-MS/MS. The results showed that among the differentially expressed proteins between AL cells and normal lymphocytes and granulocytes, some proteins involved in the process of malignant transformation (such as Op18, NM23-H1), cell proliferation (such as PCNA) and apoptosis inhibition (such as tumor necrosis factor inhibitor protein) were found to be up regulated in AL cells. However, some proteins involved in differentiation and physiological functions of normal cells were down regulated in AL cells. It is concluded that there are many events involved in the process of leukemogenesis, expression of some proteins relating to the malignant transformation, cell proliferation and apoptosis inhibition are up-regulated in AL cells. The proteome analysis may provide a new approach to explaining the molecular mechanism underlying the pathogenesis of AL.
Adolescent ; Adult ; Bone Marrow Cells ; chemistry ; pathology ; Female ; Humans ; Leukocytes ; chemistry ; Male ; Middle Aged ; Neoplasm Proteins ; analysis ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; metabolism ; pathology ; Proteome ; analysis