OBJECTIVETo investigate the effects of growth hormone (GH) on the erectile function and the number of nNOS-containing nerve fibers in internal iliac arterial ligation rats.

METHODSThirty-six mature male Wistar rats were randomized into 3 groups: GH intervention group, internal iliac arterial ligation group and sham operation group. After four weeks and eight weeks of treatment, one jalf of the rats of each group were selected respectively and tested for erectile function and then sacrificed for the detection of nNOS-containing nerve fibers by streptaridin-peroxidase immunohistochemistry techniques(SP method).

RESULTSAfter four weeks, the erection frequency and the number of nNOS-containing never fibers in the sham operation group significantly increased compared with the other two groups, which showed no significant difference(P > 0.05). There was no significant difference in erection rate among the three groups. After eight weeks, the number of nNOS-containing never fibers in corpus carvosum in the internal iliac arterial ligation group decreased compared with the other two groups, between which there was no significant difference. And there was no significant difference in erection frequency and erection rate among the three groups.

CONCLUSIONGrowth hormone can improve erectile function of internal iliac arterial ligation rats, which can be explained by the increase in the number of nNOS-containing never fibers in corpus carvosum of rats.

Animals ; Erectile Dysfunction ; drug therapy ; Growth Hormone ; pharmacology ; Iliac Artery ; Ligation ; Male ; Nerve Fibers ; enzymology ; Nitric Oxide Synthase ; analysis ; Nitric Oxide Synthase Type I ; Penile Erection ; drug effects ; Rats ; Rats, Wistar

Objective To investigate the changes in serum HP and ADMA levels in patients with ACI and the correlation of their levels with recanalization after venous thrombolysis and poor prognosis.Methods A total of 260 ACI patients undergoing venous thrombolysis in our hospital from January 2020 to March 2023 were retrospectively recruited,and were categorized into reper-fusion group(n=196)and non-reperfusion group(n=64)based on the efficacy of thrombolysis.After a 90-day follow-up,they were further divided into good prognosis group(n=159)and poor prognosis group(n=101)according to the results of a modified Rankin scale.Serum levels of HP and ADMA at admission were compared between the two groups.Logistic regression analysis was used to analyze the risk factors for non-reperfusion and poor prognosis in ACI patients.ROC curve analysis was performed to evaluate the predictive value of serum HP and ADMA levels for non-reperfusion and the diagnostic efficiency for poor prognosis in ACI patients.Results The non-reperfusion group exhibited notably elevated serum HP and ADMA levels than the reperfusion group(2.10±0.21 g/Lvs1.29±0.31 g/L,1.68±0.19 μmol/L vs 0.69±0.11 μmol/L,P＜0.01).HP and ADMA were identified as significant risk factors for uncanalization after treatment(P＜0.01).The AUC value of their combination in diagnosing uncanalization after venous thrombolys-is was 0.869(95％CI:0.830-0.908).Furthermore,significantly higher serum levels of HP and ADMA were observed in the poor prognosis group than the good prognosis group(2.27±0.19 g/L vs 1.15±0.34 g/L,1.72±0.21 μmol/L vs 0.64±0.10 μmol/L,P＜0.01).HP and ADMA were also recognized as influencing factors for poor prognosis in 90 d after treatment(P＜0.01).The AUC value was 0.816(95％CI:0.768-0.865)when their combination was used to predict poor prognosis in 90 d after treatment.Conclusion HP and ADMA are highly expressed in the se-rum of ACI patients with failed venous thrombolysis and poor prognosis.Their combined detec-tion can effectively predict both uncanalization and poor prognosis.