0.05). Positive medicine group, Qingqinye high dosage group, Qingqinye low dosage group and Qingqinye middle dosage group reduced blood ADA activity and better than model group (P

Objective:To observe the effects of zinc supplementation on the function of brain after sleep deprivation(SD).Methods:Rats SD model was made by the “Flower Pot" technique.Three days later,the rats received Y-labyrinth test to test the function of learning and memory.NADPH-d histochemistry and ABC immunohistochemistry were applied to exam the activity of NOS and the levels of protein expression of nNOS in hippocampus of rats.Results:Compared with the normal group,Learning times of rats in Y-labyrinth test of the SD group increased(89.3?25.3 /67.1?29.3,t=1.81,P0.05).Compared with the SD group,in TZC supplementation group,the levels of NOS in sub-area of hippocampus(CA_1:27.2?2.8;CA_3:15.3?1.6;DG:21.8?1.9,P0.05).Conclusions:TZC supplementation can improve learning and memory function of rats,and the mechanisms might be related to increased level of NOS and the protein expression of nNOS in hippocampus of animals.

Objective:To investigate the significance of T cells morphologic character in ascites and pleural effusion in guiding tumor therapy. Methods: Totally 60 ascites and pleural effusion specimens were collected from patients with malignant tumor and were divided into 3 groups: chemotherapy group (18 cases), immunotherapy group (25 cases) and untreated group (17 cases). Ten benign specimens were taken as control.The T cell subtypes in ascites and pleural effusion were analyzed by EnVison~TM immunohistochemistry two-step technique. The T cells were analyzed semi-quantitatively and observed morphologically. Results: The lymphocytes were scattered around tumor cells and formed into petal-like structures. Most tumor cells surrouned by lymphocytes had morphologic changes. The positive rates of CD4, CD8, CD25, CD3, CD45_~RO ,S-100 and CD34 in malignant pleural and ascites effusion of immunotherapy group were significantly higher than those of chemotherapy group and untreated group (P

To find out the method of assessing the car-diac reserve function under the resting condition,we observed the changes of left ventricular con-tractility, relaxation and compliance, as well ascardiac pump function under the resting and over-loaded conditions with intact hearts of male rats invarious states of heart failure induced by variousadriamycin cumulative doses. The results of thepresent study indicated that the degree of my-ocardium injury was aggravated along with the in-crease of adriamycin cumulative doses. Mean-while, all of the parameters of left ventricular con-tractility, relaxation, compliance and cardiac re-serve function decreased significantly. However,the indexes of basic cardiac pump function in-creased to a certain extent because of the influenceof non-cardiac factors. The discriminant analysisshowed that the degree of heart failure assessed bymeans of the discriminant function, or synthesizingthe changes of left ventricular contractility, relax-ation and compliance under the resting condition,was almost consistent with that by means of thecardiac reserve function. In addition, the multipleregression indicated that the contractility, relax-ation and compliance had different effects on thecardiac reserve function, corresponding to the different states of heart failure.

Objective To investigate the infection situation of intestinal nematodes and knowledge about the prevention and control of intestinal nematodiasis,so as to explore the effective control measures in Jingjiang City. Methods The towns where more floating people lived were randomly selected and the infection situation of intestinal nematodes was investigated with Kato-Katz method,and the residents'awareness of the prevention and control of nematodiasis was surveyed with questionnaires. Re-sults From 2013 to 2015,totally 4555 local residents and 2278 floating people were investigated in Jingjiang City. The infec-tion rate of intestinal nematodes was 0.29%(13 cases)in the local people,while the rate was 0.75%(17 cases)in the floating people,and the difference was significant(χ2=7.380,P<0.01). The differences of the intestinal nematode infection rates be-tween sexes in both local residents and floating people were not significant(χ2=0.010,0.048,both P>0.05). The awareness rate of intestinal nematodiasis prevention and control of the local residents was significantly higher than that of the floating peo-ple(χ2 =9.649-164.533,all P<0.01). Conclusion The floating people is the focus of intestinal nematodiasis control,and the health education of ancylostomiasis control should be strengthened in Jingjiang City.

BACKGROUND:Bryan cervical disc replacement and anterior cervical discectomy and fusion have a dispute in the treatment of cervical spondylosis.
OBJECTIVE:To evaluate the clinical effects of Bryan cervical disc replacement and anterior cervical discectomy and fusion by meta-analysis, thereby providing clinical evidence for treatment strategy of cervical spondylosis.
METHODS:The authors searched Medline, PubMed, EMBASE, OVID, CBM, CNKI and also searched manual y seven relevant Chinese orthopedic journals for articles pertinent to clinical research of Bryan cervical disc replacement and anterior cervical discectomy and fusion. Extracted data included the range of motion of the cervical spine, visual analog scale score, neck disability index, the Japanese Orthopaedic Association score. Meta-analysis and forest plots were conducted with RevMan4.2.2 Software.
RESULTS AND CONCLUSION:There are eight articles in the meta-analysis, including 883 patients (430 patients receiving Bryan cervical disc replacement, and 453 patients receiving anterior cervical discectomy and fusion). Meta-analysis did detect statistical y significant differences in the range of motion of the cervical spine at 3 months and 24 months postoperatively between the two groups, but did not detect statistical y significant differences in visual analog scale score, neck disability index, the Japanese Orthopaedic Association score between the two groups. These findings indicate that Bryan cervical disc replacement is superior to anterior cervical discectomy and fusion in the range of motion of the cervical spine. However, the current literature offers no evidence to support superiority of the Bryan cervical disc replacement over the anterior cervical discectomy and fusion.

Objective The aim of this study was to analyze the mechanism of degenerative spondylolisthesis (DS) through measuring vertebra arch angle and facet joint degeneration with 64-slice spiral computed tomography (CT). Methods One hundred and six patients complained of lumbodynia were examined with 64-slice CT. In 28 of 106 patients have lumbar spondylolisthesis, while the other 78 cases have not. The vertebra arch angles of all cases were measured and the facet joint degenerations were analyzed. Results L4--5 vertebra arch angle in DS group were 119. 0°±2. 2°(left) and 118. 8°±1.9° (right) ,respectively. L4--5 vertebra arch angle in control group were 102.9°±1.2°(left) and 104. 7°± 1.2° (right) ,respectively. The joint angle in DS group were 41.1°±1.2°(left) ,and 29.0°±1.2°(right), respectively. The joint angle in control group were 45.5°±2. 1°(left), and 45. 1°±1.2°(right), respectively. There were significant differences of L4--5 vertebra arch angle and more serious of facet joint degeneration( P < 0. 05 ). Conclusion L4 vertebra arch inclination and facet joint degeneration were important to diagnosis of spondylolisthesis.

Objective: To study the effect of taurine to resist the impact of lead on learning and memory. Methods: ABC immunohistochemistry method was used to study the quantity change of nNOS positive neuron in hippocampus of the rats which were fed with distinct dosage of lead acetate in drinking water (0.02, 0.2 g/L) and distinct dosage of taurine (5,10 g/kg) in feed. Rusults: Taurine could increase nNOS positive neuron quantity obviously in hippocampus of rats induced by lead lesion. Conclusion: Taurine could resist impact of lead on learning and memory obviously.

Objective: To observe the effect of 2 Hz electroacupuncture (EA) on long term depression (LTD) of synaptic transmission in the spinal dorsal horn in rats with neuropathic pain, so as to explore the central mechanisms of the antinociceptive effects of 2 Hz electroacupuncture on neuropathic pain. Methods: The neuropathic pain models were produced by tight ligation of the L5/L6 spinal nerves in Sprague Dawley rats. The C fiber evoked field potentials in the spinal dorsal horn were recorded with extracellular recording techniques. The parameters of the electroacupuncture were as follows: frequency of 2 Hz, wavelength of 0.6 ms, intensity of 1,2,3 mA lasting 10 min for each intensity, stimulation time of 30 min. The positive stimulating electrode was placed in acupoint “sanyinjiao” and the negative electrode in “zusanli”. Results: (1) 2 Hz electroacupuncture significantly decreased the amplitudes of C fiber evoked field potentials in the spinal dorsal horn in rats with neuropathic pain to (49.4?0.6)% of the control, compared with that (100.1?1.2) % of the control before EA (unpaired t test, P

OBJECTIVE: To study the pharmacological activities and toxicity of the crude alkaloids of Corydalis adunca maxim and to provide pharmacological data for further development of this herbal medicine. METHODS: The anti- inflammatory effects of the crude alkaloids of Corydalis adunca maxim were observed by using xylol and agra to induce the turgidness in the rats. The analgesic effects were observed by body distortion methods. The LD50 and 95% creditability were calculated with developed Karber Method. RESULTS: The administration of alkaloids of Corydalis adunca maxim had the function of inhibiting the auricle swelling caused by xylol and joint swelling caused by agar, and of decreasing the body- distortion of the rats. The LD50 of the crude alkaloids of Corydalis adunca maxim was 1. 833g. kg- 1 and the 95% creditability was 1. 18～ 2. 06g? kg- 1. CONCLUSIONS: The crude alkaloids of Corydalis adunca maxim has anti- inflammatory and analgesic effects in rats.