1.Biological function of IL-23
Journal of Clinical Pediatrics 2010;(3):295-296,299
Interleukin(IL),generated by immunocytes or non-immune cells,is a group of cell factors that holds many important biological effects. Up to now,with the rapid progress of molecular biology and cell biology technique,29 types(IL-1 ~ IL-29)of IL have been reported since its denomination in 1979. IL-23,one kind of interleukin found in 2000,is composed of p19 and p40 sub group. Recent studies showed that it has various biological functions,such as acting on T cell to make it produce various cell factors,acting on DC and MP to affect the secretion of the relevance cell factors. The biological functions of IL-23 have played an important role in various diseases. This review describes the molecular structure,biological function,and its effects on related diseases.
2.Laparoscopic excision of intrathoracic oesophageal duplication cyst in a Singaporean adult male.
Jirong LU ; Lynette Ma LOO ; Jimmy By SO ; Asim SHABBIR
Annals of the Academy of Medicine, Singapore 2012;41(3):129-131
Esophageal Cyst
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diagnostic imaging
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surgery
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Humans
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Laparoscopy
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methods
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Male
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Radiography
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Young Adult
3.Clinical study on pediatric Fisher's syndrome in Twenty-two cases
Jinghui SUN ; Yanyan HAN ; Li LIU ; Jirong LU
Journal of Jilin University(Medicine Edition) 2001;27(2):181-183
Objective: To confirm the pathogenesis and diagnosis of pediatric Fisher's syndr ome.Methods: Clinical manifestations and Cerebrospinal fluid (CSF) were compared and studied in 22 patients diagnosed as Fisher's syndrome.Results:Twenty- two cases of Fisher's syndrome both had the characteristics of ophthalmoplegia and cerebella ataxia,and had different degrees of incomplete fl accid paralysis in the ar ms and legs. In about half of 22 cases, the CSF showed divergence phenomenon of the protein and cell. Conclusion: Fisher's syndrome is a specific or variant type of AIP involving the cranial nerves, in which the cerebellar ataxia is taken as a main symptom and sign .
4.The role of TH1 cell in respiratory syncytial virus bronchiolitis
Jian CHANG ; Dong LIANG ; Yinbo CHEN ; Jirong LU ; Yumei LI
Chinese Journal of Immunology 1985;0(05):-
0.05).Conclusion:TH1 cell might not complicate in the pathogenesis of RSV bronchiolitis. The IL-2 levels showed a heterogenous behavior.
5.Interference of IL-4RA transfection to express level of STAT6 in airways
Guilan WANG ; Jirong LU ; Cuimei ZHANG ; Guoqing LUO
Chinese Journal of Immunology 2000;0(08):-
Objective:To study the effect of airway IL-4RA gene transfer on asthma-associated expression of STAT6 in the mice of allergic asthma.Methods:Forty BALB/c mice were randomly divided into four groups:blank control,asthma model,asthma model administered by pLNC-laz,and asthma model administered by glucocorticoid and pLNC-IL-4RA.The ten mice in each groups were sensitized by i.p.injection of 10 ?g of OVA emulsified in 40 mg of aluminum hydroxide in a total volume of 200 ?l on the days of 1st,7th and 14th.On the 15th day,the mice were challenged via airways with OVA for 7 days,20 min a day,blank control were sensitized by i.p.injection of 200 ?l pH=7.4 PBS on days 7 and 14.Asthma model was administered by pLNC-laz or pLNC-IL-4RA for 3 days beginning at the 12th day.The mice of the group with glucocorticoid was administered with glucocorticoid on the 15th day for 7 days.24 hours after the last challenge,the mice were sacrificed by bloodletting.Serum OVA-specific IgE and blood Eosinophils were measured.The lungs were fixed by immersion in 10% formalin.Expression of STAT6 was identified by immunohistochemistry.Results:It was shown that pLNC-IL-4RA was integrated to the genomic DNA of pneumonic tissues.Retrovirus-mediated delivery of IL-4RA to airways of the asthma mice weakened airway eosinophilia triggered by either IL-13 or IL-4.Furthermore,IL-4RA delivered by retrovirus was expressed in airways of the mice with allergen sensitization,resulting in significiant reduction of expressing level of asthma-associated STAT6 in the experimental mice of allergic asthma.Conclusion:Retrovirus mediated delivery of IL-4RA to airways reduces expression of STAT6 in pneumonic tissues of asthmatic mice.Thus,the gene theraphy can be a potential therapeutic option to treat and control chronic airway inflammation and asthmatic symptoms.
6.Changes of serum IL-1、IL-6 and IL-8 in children with bronchial asthma
Qingshan MA ; Wenyong FU ; Jirong LU ; Hongzi LI ; Qian WANG
Journal of Clinical Pediatrics 2001;(1):7-8,31
Serum IL-1,IL-6 and IL-8 were determined by subjects-using enzyme linked immunosorbent assay (ELISA technique) in 40 children with bronchial asthma consisting of 20 cases in the acute stage and 20 cases in the remission stage,and another 20 health children as normal control,respectively.The results showed that the levels of serum IL-1(11.9pg/ml±2.84pg/ml),IL-6(0.75ng/ml±0.3ng/ml)and IL-8(0.08ng/ml±0.05ng/ml)in the acute stage were significantly higher than those in the remission stage(8.41pg/ml±0.05pg/ml(IL-1),0.49ng/ml±0.08ng/ml(IL-6)and 0.02ng/ml±0.01ng/ml(IL-8)) and in the normal children (8.4pg/ml±0.32pg/ml(IL-1),0.4ng/ml±0.09ng/ml(IL-6)and 0.02ng/ml±0.02ng/ml(IL-8)),respectively.Further more,there were a significant positive correlation between IL-1 and IL-6 or IL-8(P<0.01).It is concluded that IL-1 may involve in the pathologic process of asthma like IL-6 and IL-8,and an inflammatory reaction may still exist in the airway during the remission stage of asthma. The imbalances of cytokine network may probably be important reason as a molecular basis in the asthma pathogenesis.It is suggested that it can be valuable to predict an acute attack of asthma when the levels of these cytokines increase.
7.Change of interleukin-23 level in mouse asthma model and its action mechanism
Yunfeng ZHANG ; Kaishu ZHAO ; Bo ZHANG ; Meng SUN ; Jirong LU
Journal of Jilin University(Medicine Edition) 2006;0(04):-
Objective To measure the change of serum interleukin-23(IL-23) level before and after treatment with glucocortcoid and analyze the correlations between levels of IL-23 and IL-4,IFN-?,IL-17,and explore the role of IL-23 in bronchial asthma.Methods Thirty femal BALB/c mice were randomly divided into three group(n=10): group A(asthma model),group B(corticosteroid treatment) and group C(normal control).All mice were killed 24 h after final OVA challenge.The blood samples were obtained for measurements of serum IgE,IL4,IFN-?,IL-17and IL-23 levels by enzyme-linked immunoabsorbent assay(ELISA).Results The mouse asthma model appeared ethological changes specific to asthma.The level of OVA specific IgE in serum increased.The level of serum IL-4 increased and the level of IFN-? decreased.Correlation analysis showed negative relationship between IL-4 and IFN-?(r=-0.859,P
8.Detection of chemokines in patients with Kawasaki disease and Henoch-Schonlein purpura and its clinical significance
Jian CHANG ; Xin ZHAO ; Dong LIANG ; Yinbo CHEN ; Jirong LU
Journal of Jilin University(Medicine Edition) 2006;0(01):-
Objective To investigate the changes and clinical significances of the chemokines of interferon-?-inducible protein-10(IP-10),monocyte chemoattractant protein-1(MCP-1) and growth-related oncogene-?(Gro-?) involved in pathagenesis of Kawasaki disease(KD) and HenochSchonlein purpura(HSP).Methods The chemokines production of IP-10,MCP-1 and Gro-? were assayed by ELISA in 15 patients with KD,12 patients with HSP and 10 healthy children.Results The plasma levels of IP-10 and MCP-1 were markedly elevated in KD group [(394.2?176.4)and(420.5?163.4)ng?L-1]compared with HSP group[(94.8?66.4)and(109.2?76.6)ng?L-1] and the control group [(76.4?46.5)and(87.7?47.8)ng?L-1](all P0.05),as well as Gro-? between the three groups.Conclusion Monocyte may enhance the immune damage in KD pathogenesis,and the levels of IP-10,MCP-1 may be important indexes for KD.Neutrophil may be not involved in pathogenesis of HSP and KD.
9.The expression of β_2 AR and IL-12 and their interaction with childhood asthma
Liang ZHOU ; Jirong LU ; Qingshan MA ; Huanji CHENG ; Zhili GUO
Journal of Clinical Pediatrics 2010;(2):142-144,155
Objective To study the relationship between asthma and mRNA expression of β_2AR and IL-12 gene;to determine the expression level of β_2AR, IL-12 related to CysLT_1-receptor;to analyze the correlation between the severity of asthma and the level of the genes expression. Methods White blood cells was drawn from peripheral blood mononuelear cells of asthmatic children. DNA was taken and related genes were analyzed for half quantitative analysis by D-congeal glue analyzer software. Results The level of IL-12. β_2AR mRNA expression in the asthma group is obviously lower than healthy controls (P < 0.01) ;when asthma attacks, the expression of IL-12 is positively correlated to that of β_2AR (r = 0.34, P = 0.001) and negatively correlated to that of CysLT_1-receptor (r = -0.92, P = 0.001), the expression of β_2AR is negatively correlated to the expression of CysLT_1-receptor (r = -0.85, P = 0.003). The express of IL-12 is not related to the severity of asthma (P = 0.16), while there was a negative correlation between the expression of β_2AR and the severity of asthma (P = 0.003). Conclusions The expression level of IL-12, β_22AR in the asthmatic children is obviously lower than normal;The expression level of IL-12 is positively correlated to β_2AR in PBMC of asthmatic children;The expression level of CysLT_1-receptor is negatively correlated to IL-12 and β_2AR in the PBMC of asthmatic children;The expression of β_2AR is negatively correlated to the severity of asthma.
10.Assessment of the quality of randomized controlled trials of delirium prevention of elderly in China
Lu YANG ; Yulin LIAO ; Langli GAO ; Jirong YUE ; Xuemei ZHANG
Chinese Journal of Practical Nursing 2015;31(18):1331-1333
Objective To assess the quality of randomized controlled trials (RCTs) of delirium prevention of elderly published in China.Methods The literatures from China National Knowledge Infrastructure(CNKI),WANFANG Data and VIP Database for Chinese Technical Periodicals were evaluated according to Cochrane collaboration's tool for assessing risk of bias and Jadad scale.Results A total of 53 RCTs were included,14 (26.4%,14/53) described radom number table used to generate the random allocation sequence,4 (7.5%,4/53) conducted experiments in a blinded manner,7 (13.2%,7/53) did not use intentionto-treat to analyse those who did not complete the study,9 (17.0%,9/53) had high risk of other bias,none described allocation concealment mechanism and blinding to participants and intercention implementers.Based on Jadad scales,the score was 1-4,average score was (2.3±0.8),19 (35.8%,19/53) were high-quality literatures.Conclusions The quality of present published literatures is not high,the further domestic studies should be designed high-quality to better improve clinical practice.