AIM: To assess the effect of indomethacin on tumor invasion in a human laryngeal cancer Hep-2 cell line in vitro. METHODS: Hep-2 cells were exposed to indomethacin at different concentrations for 48 h. Then cell growth rate, the colony formation in soft agar medium and cell mobility were examined, and monolayer invasion assay was performed to assess cell invasion index. RESULTS: Preteatment with indomethacin inhibited the colony formation of Hep-2 cells and the cell mobility, and decreased the invasion index. CONCLUSION: Indomethacin can inhibit the invasion of Hep-2 cells.

AIM: To evaluate the significance of cytokeratin19 (CK19) expression in diagnosing micrometastases in lymph nodes in patients with laryngeal carcinoma. METHODS: Forty cases of laryngeal carcinoma together with 163 lymph nodes were studied by the staining with hematoxylin and eosin(HE)and immunostaining with antibody against cytokeratin 19 (CK19) on histological sections of the primary tumor and regional lymph nodes. RESULTS: In all cases, CK19 was positively expressed in the primary tumor. Among 163 lymph nodes, metastases were confirmed by HE in 23 lymph nodes, and in 42 lymph nodes staining with immunohistochemistry, micrometastases were found in 19 lymph nodes. Micrometastases in the lymphonodes were significantly related to the T staging. CONCLUSION: Immunohistochemical staining is a valuable method for the detection of node micrometastases in patients with laryngeal carcinoma.

0.05). But typeⅢsodium channel expressed higher than that in control groups in hippocampus, restriction mapping analysis showed thatⅢN increased significantly (P

Objective To observe change of T regulatory cells (Tregs) and its relation with in-jury severity and sepsis following severe muhiple injury. Methods A total of 60 patients were em-ployed in the study and divided into severe group (30 patients) and critical group (30 patients) based on ISS scores and into sepsis group (22 patients) and non-sepsis group (38 patients) based on complication of sepsis. The proportion of Tregs in peripheral blood in different groups was detected by flow cytometry at days 1,3,5 and 8 after injury. Results The proportion of Tregs was significantly increased at day 5 postinjury, with statistical difference compared with that at day 3 postinjury (P < 0.01). The proportion of Tregs remained increasing at day 8 pestinjury (P < 0.05). At day 8 postinjury, the proportion of Tregs in critical group was significantly higher than that in severe group (P < 0.01). At the same time, the proportion of Tregs in sepsis group was significantly higher than that in non-sepsis group (P < 0.05). Spearman correlation analysis showed a positive correlation of Tregs proportion with ISS score (rs =0.654, P < 0.01). Conclusions Tregs play an important role in suppression of T cell-mediated im-munity after severe injury. The variation of Tregs can help evaluate prognosis and predict the risk of com-plicating sepsis in patients with severe multiple injury.

Objective:To observe the efficacy of Xiaochaihu Decoction combined with Tongqiao-Huoxue Decoction in the treatment of cardiac neurosis with syndrome of blood stasis and the effect on serum levels of inflammatory cytokines. Methods:A total of 84 patients with cardiac neurosis, who were admitted to Zhongtieshanqiao Group Hospital, were randomly devided intoto the observation group (42 cases) and the control group (42 cases) withthe random number table method. The control group received Betalog and Glutamine tablet. The observation group took Xiaochaihu Decoction combined with Tongqiao-Huoxue Decoction orally on the basis of the control group. Both groups were treated for 4 weeks. Hamilton depression scale (HAMD) was used to evaluate the degree of depression, Hamilton anxiety scale (HAMA) was used to evaluate the degree of anxiety, and hypersensitive c-reactive protein (hs-CRP) and tumor necrosis factor-alpha (TNF-α were measured by ELISA, and the clinical efficacy was evaluated. Results:The total effective rate of the observation group was 95.24% (40/42) andthe control group was 75.61% (31/41). The difference between the two groups was statistically significant ( χ2=4.973, P=0.026). After the treatment, scores of HAMD and HAMA of the observation group were signifcantly lower than that of the control group ( t values were 6.579, 8.941, respectively, all Ps<0.01), and scores of impatience, headache, insomnia, forgetfulness, and chest and rib pain were significantly lower than those of the control group ( t values were 7.888, 8.777, 8.836, 9.964, respectively, all Ps<0.01). After the treatment, serum levels of hs-CRP and TNF-α in the observation group were significantly lower than that of the control group ( t values were 5.847, 8.453, respectively, all Ps<0.01). Conclusions:Xiaochaihu Decoction combined with Tongqiao-Huoxue Decoction can relieve the clinical symptoms of cardiac neurosis, and down-regulate serum levels of hs-CRP and TNF-α.