Objective To detect and analyse the mutations in rhodopsin gene of members in a family affected by autosomal dominant retinitis pigmentosa (ADRP). Methods Using the polymerase chain reaction (PCR), we amplified exon 1-5 of rhodopsin gene in patients with ADRP,and analyzed it with direct sequence measuement. Results The Gly-182-Asp mutation in the rhodopsin gene was detected in most of affected members of this ADRP family, but no mutation was detected in two affected members and the control ones. Conclusion We cannot regard the Gly-182-Asp mutation in the rhodopsin gene as the pathagenic factor of the ADRP family. It is likely there is a new gene next to the rhodopsin gene.

Objective To investigate the therapeutic effects of thrombolytic drug infusion via carotid artery on experimental central retinal artery occlusion (CRAO), and observe the changes of fibrinolytic activity in the systemic circulation. Methods To dissolve the thrombi in 15 cats (30 eyes) with CRAO established by laser irradiating a branch of central retinal artery after intravenous injection of photochemical drugs, urokinase (UK) was directly infused via carotid artery in 5 cats (10 eyes) in group A or intravenously injected in 5 cats (10 eyes) in group B, and isotonic saline solution was intravenously injected in 5 cats (10 eyes) in group C respectively. The patency of the artery was evaluated by fundus fluorescein angiography. Moreover, the changes of fibrinolitic activity in the blood were observed by blood biochemical examination. Results Four hours after UK infusion, the complete repatency proportion was 80% (5 cats 8 eys) in group A, and 50% (4 cats 5 eyes) in group B. There was significant difference between the two groups. Besides, after the infusion, the indexes of coagulation, fibrinolysis, and anti-fibrinolysis in group A were better than those in group B and C (P

The mucosa associated lymphoid tissue(MALT) lymphoma represents a distinct subgroup of B cell non Hodgkin lymphomas (NHL) arising from extranodal sites. These neoplasms usually arise from the gastrointestinal tract,salivary gland,thyroid and ocular adnexal, which have been recognized from clinical,pathological and molecular genetics aspects.In this paper we reviewed the investigation progress of MALT lymphoma molecular biology.

There are some characteristics in ophthalmology teaching for students of seven-year program,such as ophthalmological theory teaching,teaching materials selection,clinical effective teaching methods,and research training methods.In view of the existing problems in current ophthalmology teaching mode for seven-year program,a series of initiatives for researching a new mode has been taken,and some achievements have been made in these fields by exploring some effective teaching methods and practice.

As a frontier branches of science,a significant progress in orbital diseases has been achieved in the last few years.In view of the existing problems in current teaching mode for experts in orbital disease and their characteristics,a series of initiatives for researching a new mode have been taken,and some achievements have been made.

MicroRNAs (miRNA), an abundant class of highly conserved small non-coding RNAs, suppress gene expression by binding to the 3' UTR of target mRNAs, which presents an entirely new approach to post-transcriptional regulation of gene expression. MiRNAs have been shown to play key roles in the regulation of diverse biological processes, such as development, inflammation, apoptosis, and tumorigenesis. Several studies have shown the dysregulated miRNAs in various lymphomas including the overexpression of miR-106a and miR-17-92 in T cell lymphoma;upregulated miR-155, miR-221 and miR-21 in diffuse large B-cell iymphoma and follicular cell lymphoma, and these miRNAs associated with subtypes of diffuse large B-cell lymphoma. Overexpression of the miR-17 cluster showed reduced levels of apoptosis, suggesting that the main effect of these miRNAs was to suppress cell death. Although miRNAs can act as both tumour suppressor and oncogenic molecules, the mechanistic explanation for this dysregulation is unclear but maybe linked to the defects in the miRNA biosynthetic machinery. As more evidence accumulates, it is likely that miRNAs will emerge as a new class of epigenetic regulator.

Objective: To study the effect of topical administration of basic fibroblast growth factor (bFGF) on healing of rabbit corneal wound caused by mustard gas. Methods: All the corneas of 8 New Zealand white rabbits were injured by liquid mustard at the concentration of 0.2%. One eye of each rabbit was treated with bFGF eye drops 6 times daily, the other eye with 0.25% chloromycetin eye drops as control. Computerized image analyzer was used to calculate the corneal epithelial healing rate. Results: The mean epithelial healing rate in bFGF-treated eyes was (1.276?0.152) mm 2/h, whereas in the control eyes it was (1.094?0.154) mm 2/h. The difference of 2 groups was statistically significant (P

300mg%). Conclusion: Acetylshikonin's content was high, radix arnebiae oil colour was very red, It was up to the mustard.

As a frontier branches of science,a significant progress in orbital diseases has been achieved in the last several years.In view of the existing problems in current teaching mode for experts in orbital diseases and their characteristics,a series of initiatives for researching a new mode have been taken,and some achievements have been made.

By relating the construction of orbital diseases’ resources bank,the clinical data became useful teaching resources.This paper introduces the construction requirements,its application in teaching for experts in orbital disease and some problems to be solved.