[Objective]To introduce the old Chinese medicine Professor FU Weimin 's experience in diagnosis and treatment of peripheral facial paralysis. [Methods]Firstly, analyze and summarize the relevant medical records. Secondly, to briefly elaborate that the professor FU Weimin believes the overall concept ,adheres to the syndrome differentiation as a guide, and how he treats peripheral facial paralysis by syndrome differentiation.[Results]He thinks that lack of righteousness, context is empty, Wei is not solid for the fundamental pathogenesis. More importantly, vein paralysis is a pathological feature. Treatment is from the wind, sputum, blood stasis and blood deficiency and clinical medication is unique. According to the course of staging , Professor FU stresses the precise discrimination and drugs have their own characteristics. Professor insists on people-oriented and makes a significant contribution.[Conclusion]Professor FU Weimin has a systematic understanding of the pathologic mechanism of peripheral facial paralysis, syndrome differentiation and clinical experience of prescription drugs and has made a good therapeutic effect in clinical application, which is worthy of popularizing.

[Objective ]Summarizing the old Chinese medicine Professor FU Weimin's experience in diagnosis and treatment of schizophrenia. [ Methods ] Learning from Professor FU as well as collating his medical case of schizophrenia. [Results] Professor FU thinks that sputum fire blinded heart is the pathogenesis of schizophrenia, with the Qi stagnation and blood stasis as the main risk factors. Therefore, during the treatment, clear heat and resolve phlegm should be a fundamental, and with relieving the depressed liver, promoting blood circulation to remove stasis and purgation. He emphasizes the precise dialectical when talking about the disease stage. In aspect of the drug matching, Professor FU uses formulae to clear heat and resolve phlegm, regulate the Qi, treat the interior excess syndrome with purgation and open the orifices to solve the external and internal diseases. He prefers to protect the stomach and has already made a good therapeutic effect in clinical application. [Conclusion] Professor FU's experience in treating schizophrenia provides TCM ideas and methods for clinical practice, which is worthy of popularizing.

Objective To explore the effects of miR-34a and the alteration of AMPK/mTOR signal pathway on angiotensin (Ang)Ⅱ-stimulated cardiomyocytes hypertrophy. Methods Neonatal rat cardiomyocytes were cultrued in vitro and were divided into 3 groups:negative control for lentivirus carrying miR-34a group (NC), AngⅡplus lentivirus carrying negative control group (AngⅡ+NC) and AngⅡplus lentivirus carrying miR-34a group (AngⅡ+miR-34a). The relative cell area was detected by confocal microscopy. The expression of miR-34a and hypertrophy-related genes (ANP and β-MHC) were analyzed by real time PCR. The AMPK/mTOR signal pathway was measured by Western blot assay. Results Compared to NC group, the relative cell area was increased in AngⅡ+NC group (P<0.05). But compared with AngⅡ+NC group, the relative cell area was decreased in AngII+miR-34a group (P<0.05). Moreover, compared with NC group, the expression of miR-34a was decreased, and the expression of hyperthophy-related genes(ANP and β-MHC) was up-regulated in AngⅡ+NC group. However, the expression of miR-34a was decreased, and the expression of hyperthophy-related genes (ANP and β-MHC) was down-regulated (P<0.05). Finally, compared to NC group, the ratio of phosphop-AMPK/AMPK was significantly induced in AngII + NC group, but the ratio of phosphop-mTOR/mTOR was significantly decreased (P<0.05). However, compared to AngⅡ+NC group, the ratio of phosphop-AMPK/AMPK was significantly decreased in AngII + miR-34a group, but the ratio of phosphop-mTOR/mTOR was significantly increased (P<0.05). Conclusion miR-34a is able to inhibit myocardial hypertrophy of neonatal rat cardiomyocytes, and its mechanism is partly carried out by the alteration of the signal pathway of AMPK/mTOR.

Objective To explore the effects of miRNA-204 targeted LC3B expression on Ang Ⅱ induced cardiomyocytes hypertrophy.Methods The primary neonatal rat cardiomyocytes served as the research objects and divided into the control group,AngⅡ group,combination-treated group 1 (cardiomyocytes were given Ang Ⅱ stimulation,meanwhile infected by negative control lentivirus vector),combination-treated group 2 (cardiomyocytes were given Ang Ⅱ stimulation,meanwhile infected by lentivirus carrying miRNA-204 overexpression vector) according to different treatments.About 48 h to 72 h after intervention treatment,the cardiomyocyte hypertrophy change was detected by confocal microscopy,the expression of miRNA-204 was analyzed by real time PCR,the protein expression of LC3B was measured by Western blot and targeted gene of miRNA-204 was demonstrated by dual-luciferase reporter assay system.Results Compared with the control group,the cardiomyocyte relative surface area in the Ang Ⅱ group was significantly enlarged,the protein expression of LC3B was significantly increased,the expression of miRNA-204 was upregulated,the differences were statistically significant (P＜0.05).Whereas comparing the combination-treated group 1 with combination-treated group 2,the protein expression of LC3B in the latter was down-regulated and the cell area was reduced (P＜0.05).The further luciferase activity report gene experiment results suggested that miRNA-204 was able to bind to LC3B 3'-UTR and decreased the luciferase activities (P＜0.05),but not to bind its mutated fragment for inactivating luciferase activity(P＞0.05).Conclusion miRNA-204 is able to inhibit Ang Ⅱ induced cardiomyocytes hypertrophy,its action is realized by targeting the expression of LC3B.

OBJECTIVEThis paper is aimed to explore the adverse reaction condition of Shuanghuangli an injection with three common used signal detecting methods based on SRS database of Jiangsu province, and to evaluate the performance of three methods.

METHODThree methods would be used to detect the signals based on the SRS database of Jiangsu province. Consistency of the results of these three methods with that proved in descriptions was evaluated by Kappa test. The trend graph of the confidence intervals of several time points was used to demonstrate the trend of the signal.

RESULTThe PRR method was consistent with ROR method in high degree in any situation. The results of BCPNN method was close to PRR and ROR method only when the related report count was larger. PRR and ROR methods had higher false positive rate than BCPNN method.

CONCLUSIONPRR or ROR method is proposed for signal detecting when the report count is large. BCPNN method is proposed for trend demonstration of signal with graph.

Adverse Drug Reaction Reporting Systems ; Data Mining ; methods ; Drugs, Chinese Herbal ; adverse effects ; Humans