Immune system is the autologous defence system to resist disease.αβ TCR+ CD3+ CD4-CD8-NK1.1-double negative T cell is a special T lymphocyte which has been described in both humans and rodent models.In recent years,the prognosis has been made in the mechanisms by which DN Treg target antigen-specific T cells and tumor cells.DN Treg cells play an important role in immune regulation,immunosuppression,autoimmune and resistance to the host reaction,HIV infection and antitumor.The study mainly introduces the advance of DN Treg cells in immune treatment.

DNT cells(CD4-CD8-double-negative T cells)ale newly discovered immune cells,whose surface ex press neither CD4 nor CD8 molecules.In recent years,the prognosis and survival quality of tumour patient have been greatly improved through cell immunotherapy.Immunocyte can kill the tumour directly Or through immunoregu lation.The study of DNT cells(double-nnegative T cell)on cancer cells will be of vital significance.

Objective To study the effect of enteral nutrition(EN) and total parenteral nutrition(TPN)on patients with severe acute pancreatitis by a Meta analysis.Method s Randomized controlled trials of EN in patients with severe acute pancreatitis were searched in Cochrane library,Pubmed database,CNKI database,CBM database.Data were searched by two statisticians.RevMan 5.0 was applied for statistical analysis.Results Nine randomized controlled trials were enrolled into this analysis.Meta-analysis indicated that the use of enteral nutrition decreased the incidence of infectious complications(RR was 0.37,95% CI[0.18,0.77]),pancreas infectious complications (RR was 0.44,95% CI [0.24,0.81],P ＜ 0.01),MODS (RR was 0.33,95% CI[0.19,0.56]),mortaily (RR was 0.45,95% CI [0.26,0.80]),surgical interventions (RR was 0.51,95% CI [0.36,0.71]).When starting within 48 h of admission,EN,in comparison with TPN,resulted in a statistically significant reduction in the risk of MODS,mortaily and infectious complications.Conclusion For patients with severe acute pancreatitis,early EN therapy is better than TPN.

Many alimentary tumor patients are characterized by malnutrition resulting from various elements and disorders of metabolism.So it is necessary to combine together with parenteral nutrition(PN) and enteral nutrition(EN).It can improve the nutritional status and reduce morbidity and mortality in patients with alimentary tumors in perioperative period.

Chronic inflammation is proved to play an important role in promoting carcinogenesis.Many factors in inflammatory microenvironment,such as inflammatory immune cells,pro-inflammatory cytokines,inflammatory mediators,and aberrantly activated transcription factors of pro-inflammatory pathway (NF-κB,STAT3),have been found to provide a microenvironment for lung neoplastic processes,and promote the lung carcinogenesis.On the other hand,many anti-inflammatory agents have been shown having inhibitory effects on lung tumorigenicity.With further understanding of the molecular links between inflammation and lung tumorigenesis,anti-inflammation would provide alternative strategies for prevention and therapy of lung cancer.

Objective To set up a method for determining the content of anthraquinone in Liuwei Nengxiaosan.Methods The chromatogram column was Eclipse XDB C18 (150 mm×4.6 mm, 5μm), the mobile phase composition was methanol-0.1% orthphosphoric acid (88∶12), the detection wavelength was at 254 nm, the column temperature was 30℃, the flow rate was 1.0 mL/min. Results The linear range of aloe emodin, rhein, emodin, chrysophanol and physcion was 0.076-0.380μg (r=0.999 6), 0.068-0.340μg (r=0.999 8), 0.076-0.380μg (r=0.999 9), 0.070-0.349μg (r=0.999 9), 0.071-0.355μg (r=0.999 7), respectively. The average recovery rate respectively was 97.88% (RSD=0.72%), 97.52% (RSD=0.96%), 98.79% (RSD=0.95%), 97.77% (RSD=1.18%), 96.34% (RSD=2.00%).Conclusion The method is highly effective, quick and sensitive, suitable for quality control of Tibet traditional medicine Liuwei Nengxiaosan.

This study examined the effects of a recombinant adenovirus Ad-PTEN-EGFP on the proliferation of A549 cells, a human lung carcinoma cell line, in vitro and on the growth of the implanted tumors in the nude mice in vivo, explored the underlying mechanisms and evaluated the in vitro transfection efficiency of Ad-PTEN-EGFP into A549 cells. The expression of Ad-PTEN-EGFP in the A549 cells was determined. The proliferation and the apoptosis rates of the A549 cells with Ad-PTEN-EGFP transfection or not was detected by MTT and flow cytometry. Ad-PTEN-EGFP at different doses was injected intratumorally to the tumor-bearing mice induced by the A549 cells. Tumor sizes were measured on an alternate day. After all the mice were sacrificed, the implanted tumors were removed for routine histological examination, weight test, HE staining and immunohistochemical staining. The expressions of Bax, P16 and P53 in the tumor tissues and those of caspase-3, CD34 and VEGF in the mouse sera were detected. Tumor cell apoptosis was measured by TUNEL method. The results showed that the vitality of the A549 cells after transfection with Ad-PTEN-EGFP declined. The expression of green fluorescent protein was observed under fluorescent microscope. The transfection rate was in excess of 50%. The mRNA and protein expression of PTEN in the transfected cells was confirmed. The proliferation rate of the transfected cells was significantly decreased when compared with that of the non-transfected cells (P<0.05). The number of the apoptosis cells was increased in the transfected cells (P<0.05). The models of implanted tumors were successfully established by injection of the A549 cells in the flank of Balb/c nude mice. Administration of Ad-PTEN-EGFP to the tumor-bearing nude mice resulted in a suppression of tumor growth. There were statistically significant differences in the tumor weight and tumor volume between the Ad-PTEN-EGFP-treated group and the control groups (P<0.05). In contrast to those in the control groups, tumor tissues in the Ad-PTEN-EGFP-treated group were shown to have typical extensive vacuolar degeneration and massive hemorrhagic necrosis. Apoptotic bodies were also observed in the tumor cells. The expressions of Bax, caspase-3 and P16 were increased (P<0.05) while those of CD34, VEGF and P53 decreased (P<0.05) in the Ad-PTEN-EGFP-treated group. It is concluded that Ad-PTEN-EGFP could induce the apoptosis of the A549 cells and inhibit their proliferation. And it could also substantially suppress the tumor growth in the tumor-bearing nude mice and induce apoptosis of the tumor cells as well. These findings carry significant implications for adenovirus vector-based PTEN gene therapies for lung cancers.

2-Aminoadipic Acid ; analogs & derivatives ; analysis ; Aldehyde Dehydrogenase ; genetics ; Anticonvulsants ; therapeutic use ; Biomarkers ; analysis ; Brain ; pathology ; DNA Mutational Analysis ; Electroencephalography ; Epilepsy ; diagnosis ; drug therapy ; genetics ; Genetic Association Studies ; Humans ; Infant ; Infant, Newborn ; Magnetic Resonance Imaging ; Mutation, Missense ; Prognosis ; Pyridoxine ; therapeutic use ; Seizures ; diagnosis ; drug therapy ; genetics ; Status Epilepticus ; diagnosis ; drug therapy ; genetics

Objective To investigate the relationship between cysteinyl leukotriene and clinical response to antileukotriene treatment,and to help select a clinical pharmacologic scheme.Methods Seventy-eight cases with acute mild-moderate asthma were treated with montelukast in a four-week trial.Asthmatic symptom score,usage of ?_2 receptor agonist,percentage of eosinophil,serumal IgE concentration,spirometry and urinary leukotriene E_4(uLTE_4)were measured pre- and post-treatment.Logistic analysis was used to access the various clinical parameters correlated with the response to montelukast.Results There were 48 responders and 30 nonresponders.The uLTE_4 level from the responders was higher than that of nonresponders(P0.05).Subjects with a uLTE_4 level of ≥1 200 pg/mL were11.5 times more likely to respond to montelukast than those with

Objective To investigate the relationship between the number of eosinophil(Eos)and level of eosinophil cationic protein(ECP)in induced sputum and asthma severity and their value of differential diagnosis.Methods From July 2002 to June 2004,59 asthmatic patients were selected in the People Hospital of Wuhan University.The number of eosinophil and level of ECP were measured by Wrights' stain and Immuno-CAP System.The lung function was also evaluated.The same index was measured in 20 patients with COPD and 10 healthy subjects as control.Results The number of eosinophil in induced sputum in asthmatic patients negatively correlated with FEV_1%(r=-0.65,P