1.Comparative proteomic analysis on radioresistant nasopharyngeal carcinoma cell
Xiaodong ZHU ; Shiting HUANG ; Song QU ; Fang SU ; Ya GUO ; Jinzi WANG
Chinese Journal of Radiological Medicine and Protection 2012;32(3):245-248
Objective To discover radioresistance-associated proteins by performing comparative proteomic analysis on nasopharyngeal carcinoma cell lines.Methods The total proteins were extracted from radioresistant human nasopharyngeal carcinoma cell line CNE-2R and its parental cell line CNE-2,respectively.These proteins were separated by high quality two-dimensional polyacrylamide gel electrophoresis (2-DE) and then the 2-DE profiles were screened for differentially expressed protein spots by the Image Master 5.0 software.Those spots were identified by a matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.Results 32 significantly differentially expressed protein spots were screened in two different radiosensitivity cell lines and 11 proteins were identified by tandem mass spectrometry,among which 3 proteins were up-regulated in radioresistant human nasopharyngcal carcinoma cell line CNE-2R and the other 8 proteins were down-regulated.Conclusions The differentially expressed proteins of nasopharyegeal carcinoma cells with different radiosensitivity were mainly involved in apoptosis regulation,DNA damage and repair,cell cycle regulation,RNA transcription,cell signaling,cytoskeleton formation and radiation stress responses.
2.Diagnostic value of dynamic contrast-enhanced MRI in the staging of endometrial cancer
Ting ZHANG ; Ailian LIU ; Meiyu SUN ; Heqing WANG ; Ping PAN ; Jinzi XING ; Qingwei SONG
Chinese Journal of Radiology 2013;47(10):898-902
Objective To explore the relationship between the characteristics of dynamic contrastenhanced MRI (DCE-MRI) and the stages of endometrial cancer.Methods A retrospective analysis of DCE-MRI manifestation in 55 patients with scratch-pathologically confirmed endometrial cancer was performed.All patients were divided into different groups according to differentiation,muscle layer infiltration,cervix involvement and metastasis.The enhanced rate,including the arterial phase relative signal increase ratio (ARSI%),the maximal relative signal increase ratio (MRSI%) and the signal enhancement ratio (SER%),was calculated at different time (i.e.16 s,32 s,48 s,64 s and 300 s),respectively.Time-intensity curves (TIC) of lesions were also obtained and divided into 4 subtypes.Two sample t test was performed to compare the MRSI% among groups,and the Mann-Whitney U test was performed in ARSI% and SER% as well.Results The early phase enhanced rate of normal tissue was 894 ± 355,higher than that of tumor 716 ± 215 (t =-2.911,P < 0.01).The delayed phase enhanced rate of normal tissue was 1111 ± 289,higher than that of tumor 803-± 289 (t =-4.926,P < 0.01).ARSI% of low differentiation group (n =16,M =1.07%) was higher than that of middle to high differentiation group (n =39,M=0.36%).ARSI% of deep muscle layer infiltration group (n =19,M =0.76%) was higher than that of no muscle layer infiltration group (n =36,M =0.32%).ARSI% of cervix involvement group (n =27,M =0.84%) was higher than that of no cervix involvement group (n =28,M =0.25%).SER% of cervix involvement group (n =27,M =2.90%) was higher than that of no involvement group (n =28,M =1.40%) and SER% of parauterine involvement and metastasis group (n =7,M =2.03%) was higher than that of uterine involvement group (n =48,M =1.60%).The differences between these groups were statistically significant (Z =-2.038,-2.260,-2.172,-2.695 and-2.621 respectively,P < 0.05).Based on the TIC types,type Ⅰ occurred in 13 tumors (23.6%),type Ⅱ in 22 tumors (40%),type Ⅲ in 9 tumors (16.4%),and type Ⅳ in 11 tumors (20%).Conclusions Based on TIC curves,most of endometrial cancers showed significant enhancement which reflected of their rich blood supply.The parameters of DCE-MRI is helpful in staging of uterus tumors.
3.Influence of Obesity on Rehabilitation of Stroke Patients
Qiang WANG ; Rong SUN ; Tong ZHANG ; Xiaoxia DU ; Jinzi LICHAO ; Lin XIAO ; Luping SONG
Chinese Journal of Rehabilitation Theory and Practice 2015;21(5):557-561
Objective To study the influence of obesity on outcome of rehabilitation for the hemiplegic post stroke. Methods 118 hospitalized stroke patients were divided into the normal weight, overweight and obesity groups according to the Body Mass Index. All the patients received rehabilitation for 12 weeks. They were assessed with National Institute of Health Stroke Scale (NIHSS), Fugl-Meyer Assessment (FMA), Berg Balance Scale (BBS) and modified Barthel Index (MBI) before and after rehabilitation. Results The scores of NIHSS, FMA, BBS and MBI improved significantly after rehabilitation in all the groups (P<0.001), and improved the lest in the obesity group in the scores of NIHSS, BBS and MBI (P<0.05). There was no significant difference in FMA score among groups (P>0.05). Conclusion Obesity influences the outcome of rehabilitation, results in poor functioning.
4.Comparative test on puncture coring of two different needles used with the implantable drug-supplying device.
Min WAN ; Ping WU ; Jinzi SONG ; Xin YU ; Pengtao MOU
Chinese Journal of Medical Instrumentation 2010;34(6):445-447
A comparison test on the number of puncture coring is conducted using the normal needle and the Huber needle in the injection area of the implantable drug-supplying device separately. The result indicates that the number of coring using the Huber needle is much less than that using the normal needle during the puncturing. So it is suggested to popularize the Huber needle in the drug transfusion of the implantable drug-supplying device.
Infusion Pumps, Implantable
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classification
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Needles
5.Pay attention to physical test and drafting product standards of the centrifuge apparatus.
Hui XU ; Yufei JIA ; Haixin LI ; Jinzi SONG
Chinese Journal of Medical Instrumentation 2010;34(2):126-128
Compare and analyze the standards related to centrifuge apparatus and make corresponding suggestions in allusion to the problems existed in the test method and physical performance during product standard drafting process.
Blood Component Removal
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instrumentation
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Centrifugation
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instrumentation
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standards
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Device Approval
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standards
6.Research for the test method of polypropylene fiber liquid filtration membrane particles shedding.
Jinzi SONG ; Yufei JIA ; Yulian CHAI ; Bingcheng SUN ; Haixin LI
Chinese Journal of Medical Instrumentation 2011;35(6):446-448
Through five experiments, such as "Circle", "square", "side that does not shake", "burning edge", "filter", this paper discussed the effect of different experimental methods of polypropylene fiber liquid filtration membrane particles shedding. The results show that: the particles falling off the edge of the polypropylene fiber Double-sided liquid filtration membrane are very important and must be considered off the evaluation of particle pollution.
Equipment Failure
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Filtration
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instrumentation
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methods
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Particle Size
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Polypropylenes
7.Ethical Enlightenment of Foreign Rare Disease Treatment Guarantee System to China
Zezhao WANG ; Xiaolin SONG ; Jinzi ZHANG ; Mei YIN ; Ping WANG
Chinese Medical Ethics 2022;35(10):1088-1093
By summarizing the policies and regulations of foreign countries on rare disease research and drug research and development, referring to the relevant policy support of treatment guarantee system in the development of rare diseases abroad, and combining with China’s national conditions, this paper analyzed the feasible points that China can learn from, and then put forward some suggestions on the ethical problems existing in the current rare disease research, so as to provide reference suggestions for the relevant policy formulation of rare disease treatment guarantee in China: adjusting regional differences based on the principle of fairness, and establishing a national rare disease information collection center; improving the ethical quality of scientific research in pharmaceutical companies, and strengthening ethical supervision; and the government and society fulfill their obligations to protect the rights and interests of patients with rare diseases.
8.Ethical Enlightenment of Foreign Incentive Mechanism for Orphan Drugs Research and Development to China
Jinzi ZHANG ; Xiaolin SONG ; Zezhao WANG ; Ping WANG ; Mei YIN
Chinese Medical Ethics 2022;35(9):971-977
There are a large number of patients with rare diseases in China, and most of them are faced with problems such as no medicine to cure, and have drugs outside of China but not inside. This paper combed the incentive policies of orphan drugs in the United States and the European Union, including orphan drugs legislation and setting up special management institutions, orphan drugs qualification certification, government funding, tax reduction and agreement assistance in the research and development process, providing accelerated listing channels in the examination and approval process, giving market monopoly period in the circulation stage, and giving priority to review. On this basis, it also explored the incentive measures of non-profit organizations in the research and development of orphan drugs, including providing financial support, clinical research and so on. From the perspective of guaranteeing the right to life and health of patients with rare diseases, the fairness and accessibility of medication, and the subjects’ right to know and privacy in clinical trials, this paper provides reference for perfecting the incentive mechanism of orphan drugs research and development in China.