Objective:To study the characteristics of normal adult human nasal chondrocytes cultured in vit-ro. Method :The adult human nasal septal chondrocytes were cultured in vitro. The changes of cellular morpholo-gy from primary to subculture were investigated ;to observe the proliferation of chondrocytes by the growth curveand the survive rate of chondrocytes;and to determine the biologic synthesis of the chondrocytes by toluidineblue,alkaline phosphatase staining and type Ⅱ collagen immunohistochemistry reaction. The survive rate of thenasal septal chondrocytes was observed after being frozen (－196C). Result: The morphology of the primarychondrocytes maintained the spherical shape ,and some became polygonal shape after 4 days. Most of the chondro-cytes transformed into the shape of the fibroblast cell since the fifth generation. The number of the chondrocytes(2th generation)after being cultured in vitro for 5 days was approximately 4 fold of that of the plating. The phe-notype of the chondrocytes was maintained in the first 4 generation,the survive rate of the chondrocytes suscitat-ing from being frozen (－196C ) was 93 %. Conclusion:The normal adult nasal septal chondrocytes cultured inmonolayer in vitro maintained the specific chondrocytes phenotype in the first 4 generation,the growth was nor-mal ,and could survive in the condition of deep-frozen.

Objective To investigate the clinical outcome of large decompressive craniectomy in treatment of severe traumatic brain injury combined with herniation of brain.Methods A retrospective analysis was carried out to assess the clinical outcome of large decompressive craniectomy for 98 patients with severe traumatic brain injury combined with herniation of brain enrolled from Jan.2007 to Sep.2010.Results According to the Glasgow outcome scale,there were 27 patients ( GOS 5 points) with good recovery,23 ( GOS 4 points) with moderate deficit,28 (GOS 3 points) with severe deficit,6 (GOS 2 points) under persistent vegetative status and 14 (GOS1 points) deaths at the end of 6 month post injury.We observed a good prognosis rate (good recovery and moderate deficit) of 51％ and a poor prognosis of 49％.Conclusion Large decompressive craniectomy can significanty improve the outcome and reduce complications of patients with severe traumatic brain injury combined with herniation of brain.

BACKGROUND: Transforming growth factor beta 1 (TGF-β1) is a first selected growth factor in study of articular cartilage tissue engineering. Suitable concentration can stimulate proliferation, division and differentiation of articular chondrocytes. OBJECTIVE: To establish an special inducing system for differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocytes in contained TGF-β1 culture in vivo,and to observe the changes in cell form and phenotype.METHODS: BMSCs were separated and purified from rabbit tibial tubercle using attachment culture method. Surface antigen of the third-generated BMSCs was determined by using flow cytometry. Subsequently,the third-generated BMSCs were induced with TGF-β1-contained special inducing system in 21-day culture, which was compared with human septal cartilage cells of nose following induction. Collagen type Ⅱ was qualitatively determined using immunohistochemical method.RESULTS AND CONCLUSION: Rabbit BMSCs were separated and purified using attachment culture method. The third-generated BMSCs were positive for surface antigen CD44,but negative for surface antigen CD34 and CD45. Cell form was irregular 21 days after induction. Immunohistochemical staining for type II collagen demonstrated positive cells. Results suggested that under culture system containing TGF-β1 BMSCs may directionally differentiate into chondrocytes,and there were not significant differences with normal chondrocytes.

BACKGROUND: Embryonic stem cells or bone marrow mesenchymal stem cells possess potential to treat cartilage defects, but the practicability is restricted by many factors. Therefore, it is significant to explore a new source of cells.OBJECTIVE: To investigate the feasibility of differentiating human umbilical cord blood stem cells into chondrocytes. METHODS: Human mesenchymal stem cells was obtained from term delivered umbilical vein, labeled by BrdU, and co-cultured with rabbit chondrocytes using Transwell method. The harvested cells were identified by immunocytochemistrical analysis. RESULTS AND CONCLUSION: The human umbilical cord blood stem cells were differentiated into chondrocytes under induction of rabbit chondrocytes, cytoplasm of which were stained into purple, and cellular nucleus was yellow stained with BrdU. The results revealed that human umbilical cord blood stem cells can in vitro differentiate into chondrocytes.

Objective To investigate the expression of CRKL in thyroid papillary micro-carcinoma and its clinical significance.Methods 120 patients with thyroid tissue specimens were collected,in which 30 cases of diam-eter >1 cm of papillary thyroid carcinoma,30 cases of thyroid papillary micro-carcinoma,30 cases of nodular goiter, 30 cases for specimen of thyroid disease patients without diabetes.Immunohistochemical(SP)method was used to de-tect samples in CRKL expression.Results In thyroid papillary micro-carcinoma and thyroid papillary cancer group, CRKL expression positive rates were 30.12% and 29.87% respectively,which were higher than that of nodular goiter and normal thyroid group 30.03% and 28.57%(χ2 =52.102,P <0.05);The average absorbance(A)value in thy-roid papillary micro-carcinoma and thyroid papillary carcinoma group which were respectively (0.516 ±0.100)and (0.496 ±0.201),were higher than that in nodular goiter and normal thyroid group (0.246 ±0.050)and (0.117 ±0.015),the difference was statistically significant(F =149.105,P <0.05).Conclusion CRKL is highly expressed in papillary thyroid micro-carcinoma and the clinical detection of CRKL is helpful to determine the surgical plan for papillary thyroid micro-carcinoma.

Objective To evaluate availability and outcomes of using anterior half of the peroneus longus tendon (AHPLT) in knee ligament reconstruction as an autograft source.Methods From July 2007 to January 2008,100 patients with knee ligament injuries were recruited in this study.There were 33 males and 67 females aging from 16 to 62 years (mean,32.3 years).49 cases had undergone medial patellofemoral ligament reconstruction,19 cases multiligament reconstruction,18 cases double-bundle posterior cruciate ligament (PCL) reconstruction and 14 cases double-bundle anterior cruciate ligament (ACL) reconstruction.AHPLT was used as sole (49 cases) or part (51 cases) of reconstruction materials.One-incision or two-incision striping techniques were adopted to harvest AHPLT.Ligaments were fixed with screws.Post-operative assessments included Kujala knee score,Lysholm knee score,Marx knee score,International Knee Documentation Committee (IKDC) subjective evaluation form and objective evaluation grade,the Foot and Ankle Disability Index (FADI) and the American Orthopedic Foot and Ankle Society (AOFAS) scale.Results 92 cases were followed up for more than 24 months.Postoperative Kujala score,IKDC subjective score,Lysholm score and Marx score were improved significantly in all four groups of patients.According to IKDC objective evaluation grade,the number of patients reaching Grade A (normal) or Grade B (near-normal) in multiligament,PCL and ACL reconstruction were 17,15 and 12,with an excellent rate of 89.5％ (17/19),93.7％ (15/16) and 100％ (12/12),respectively.Preoperative and postoperative AOFAS scores were 97.4±2.0 and 97.2±1.6,respectively,while the FADI scores preoperatively and postoperatively were 96.8±2.2 and 96.9±2.5,respectively.These results had no statistical significance.No signs of peroneal nerve injury or peroneus longus tendon rupture was found.Conclusion It is acceptable to use AHPLT as an autograft due to its feasibility to harvest,good clinical outcome,and low rate of donor site morbidity at a minimum of two-year follow-up.

Objective:To investigate the expressions of CD44v3 and CD54 in oral premalignant epithelia and its significance in the carcinogenesis of oral mucosa.Methods:The expressions of CD44v3 and CD54 in 19 cases of normal mucosa and mucosal hyperplasia,22 case of dysplastic epithelia,26 cases of oral cancer mucosa were detected by using immunohistochemistry.Results:CD44v3 and CD54 proteins showed no expression or micro-expression in normal and hyperplastic epithelia.The expressions increased in the dysplastic epithelia and mucosa of oral cancer gradually.The difference between the expressions of the three groups was significant(P

Objective: To study and evaluate the clinical effect of emergency primary resection and anastomosis in the treatment of obstructive left hemicolon carcinoma and the value of preventing reoperation. Methods: From February 1st 2013 to February 1st 2018, a total of 46 patients with obstructive left hemicolon cancer underwent surgical treatment were selected in the First People's Hospital of Jinzhong.According to the random digital table method, 46 patients with obstructive left colon cancer were randomly divided into two groups, with 23 patients in each group.The control group received stage colon resection and anastomosis, and the observation group received stage I resection and anastomosis.The operative condition, postoperative recovery, anastomotic leakage, therapeutic effect of intestinal obstruction, serum inflammatory cytokines and reoperation rate were compared between the two groups. Results: There were no statistically significant differences in the number of lymph nodes dissection, the amount of blood lost during operation and time of operation (all P>0.05). The recovery time of anal exhaust[(2.34 ±0.86)d], the removal time of drainage tube[(4.36±1.52)d], the active time of getting out of bed [(3.81±1.17)d], the hospitalization time[(12.05 ±2.73)d] in the observation group were significantly shorter than those in the control group[(3.29±1.05)d, (6.17±1.84)d, (5.24±1.43)d, (15.76±3.69)d](t=3.357, 3.637, 3.712, 3.876, all P<0.05). The incidence rate of anastomotic fistula in the observation group was not significantly higher than that in the control group (P>0.05), and there was no statistically significant difference in the total effective rate of intestinal obstruction treatment between the two groups (P>0.05). The levels of serum inflammatory cytokines such as C-reactive protein, interleukin-6 and tumor necrosis factor-alpha in the two groups were significantly lower than those before operation(all P<0.05), but there were no statistically significant differences between the two groups before and after operation (all P>0.05). The rates of reoperation in the observation group and control group were 8.70% and 4.35%, respectively, and there was no statistically significant difference between the two groups (P>0.05). Conclusion Stage I resection and anastomosis can be used to treat intestinal obstruction effectively, dissect lymph nodes, suppress inflammatory reaction, it is safe and reliable, and do not increase the risk of reoperation.Compared with stage I resection and anastomosis, it has more advantages in accelerating postoperative recovery, which is beneficial to reduce the cost of treatment and reduce the economic burden of patients.

Objective:Metabolomics was used to analyze the dynamic changes of endogenous metabolites in serum and lung tissue of rats treated with water elution section of Siegesbeckiae Herba (SWES), and to explore the possible mechanism of lung injury and to search for the sensitive markers in serum and lung tissue. Method:SD rats were randomly divided into three groups, namely the normal group (normal saline), SWES high-dose group and SWES low-dose group (0.500, 0.125 g·kg^-1·d^-1). SWES was given for 4 weeks and stopped for 2 weeks. The weight and pathological examination were regarded as observation parameters. Serum and lung tissue samples were detected by nuclear magnetic resonance hydrogen spectrum (¹H-NMR). The metabolites in rats were analyzed by partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Result:Lung inflammation was shown in SWES high-dose group and returned to normal after withdrawal for 2 weeks. The metabolic spectrum of SWES high-dose group was significantly different from the normal group. There were 11 metabolites were identified by ¹H-NMR metabolomics, four differential metabolites were identified in serum [acetate, trimethylamine oxide (TMAO), glycine, myo-inositol] and nine differential metabolites were identified in lung tissue [lactate, acetate, phosphatidylcholine (PC), pyruvate, dimethylamine, glutamate, glycerophosphocholine (GPC), glycine, xanthine]. Lung injury was related to the disorder of pyruvate metabolism, glutamate metabolism and other pathways. Most of the metabolites in lung tissue had obviously levels of callback after drug withdrawal, which coincided with the pathological examination. Conclusion:The lung is one of the damaged organs caused by SWES, and the lung injury is reversible and may be related to energy metabolism and oxidative stress.