This study is to investigate the protective effect of rosiglitazone (RSG) against learning and memory impairment of APP/PS1/tau transgenic mice. AD mice model was replicated by using 6-month APP/PS1/tau transgenic mice. The learning and memory ability of mice was evaluated by Morris water maze and Western blotting assays was applied to measure the phosphorylation and O-glycosylation of Tau and neurofilaments (NFs) protein. The results demonstrated that RSG could reverse the learning and memory deficits of 3 x Tg mice significantly. It was also found that RSG could suppress the hyperphosphorylation of Tau and NFs protein levels and increase the glycosylation expression of Tau and NFs proteins in 3 x Tg mice brain. Together, RSG ameliorates cognitive impairments of 3 x Tg mice via the alleviation of the hyperphosphorylated Tau and NFs proteins burden in the brain.

Objective To explore the effectiveness of whole genome amplification technology in DNA typing of trace samples.Methods Simulated trace samples which contain 1～20 cells were prepared by micromanipulation.Whole genome amplification was added before conventional PCR-STR typing step,to compare the effectiveness of PEP and MDA in DNA typing of trace samples from four aspects i.e.allele imbalance,allele drop-out,locus drop-out and pseudo allele (which contains the stutter peak).Results Amplification efficiency of MDA was higher than PEP method,but allele drop-out and pseudo allele were more frequently detected.Correct DNA typing rate of PEP is higher than MDA method,however,advantaged amplification of small fiagments DNA is more obvious.Conclusion MDA method is not suitable for the current STR typing.When the absolute amount of trace samples is quite small,we couldconsider using the PEP method to enhancethe sample quantity to meet the requirement of repeat testing.At the same time it could encounterthe failure of the large DNA fragments.

Blepharophimosis ; diagnosis ; genetics ; Humans ; Male ; Middle Aged ; Pedigree ; Phenotype ; Skin Abnormalities ; diagnosis ; genetics ; Urogenital Abnormalities