OBJECTIVE To study the effect of using Beclomethasone Dipropionate (BDP)nasal spray on seasonal perennial allergic rhinitis. METHODS To 100 clinics indeed patients was divided into random for three groups:Cetirizine Hydyrochloeide Tablets cure 30 of set,take orally Cetirizine Hydyrochloeide Tablets 10 mg,everyday once; The BDP nasal spray 30 of set,spraying fog to BDP nasal spray, everyday 2 times,every time two per nostril(or everyday 3-4 times,every time a per nostril),dosage everyday can't over eight(400?g);Cetirizine Hydyrochloeide Tablets add BDP nasal spray to unite to cure 40 of set,using the medicine method together up.Cure time is a 7-14 days,have four times at least with visit the register, distinguish after treatment the 1 hour,the 7 days,the 14 days. Advertise for to carry on the valuation to account the cent to the nose department and a symptom,body of non-nose with observer from the sufferer, according to get a goal how much carry on the curative effect evaluation and covariances to learn the processing. RESULTS Cetirizine Hydyrochloeide Tablets and BDP nasal spray to have no obviously bad reaction towards cure the seasonal allergic rhinitis is all valid. Cetirizine Hydyrochloeide Tablets add BDP nasal spray to unite to cure a curative effect to equal the BDP nasal spray a treatment set but hight in Cetirizine Hydyrochloeide Tablets cure the set,through the x~2 examination,the difference has to statistics to learn the meaning. CONCLUSION Put together the function unite that the antihistamine and glucocorticoid,is the most valid under medical treatment the seasonal allergic rhinitis of method. Ideal treatment medicine is sine can cure the acute reaction symptom, and then cure the late hair reaction symptom.

Adolescent ; Asthma ; therapy ; Child ; Humans ; Quality of Life ; Respiratory Therapy ; methods ; Surveys and Questionnaires ; Treatment Outcome

Objective An HPLC-MS/MS method was established for the simultaneous determination of 7 components in Qingkailing Oral Liquid.Methods The assay was performed on a Waters ACQUITY UPLC BEH C18 column(2.1 mm×10 mm,1.7 μm)and the sample was eluted with a gradient mobile phase containing 10 mmol·L-1 of ammonium acetate and 0.1%of formic acid in water(A)-methanol(B).The mass spectrometry was carried out by electrospray ionization(ESI)with positive/negative ions in multiple reaction monitoring(MRM)mode for quantitative analysis.Results The linear ranges of adenine,chlorogenic acid,caffeic acid,geniposide,baicalin,hyodeoxycholic acid and cholic acid were 0.100 4-3.213,0.784 5-8.982,0.998-3.194,0.622 5-19.92,25.05-300.6,2.513-30.15 and 7.775-93.30 μg·mL-1(r≥0.999 0).The average recoveries(n=6)were 100.9%,98.74%,101.2%,100.2%,100.8%,99.97%and 98.94%with RSD of 1.58%,0.59%,1.78%,1.25%,0.65%,1.69%and 1.07%.The contents of the above mentioned 7 components in 15 tested samples were in the ranges of 0.12-0.18,0.19-0.24,0.06-0.09,0.34-0.37,4.54-4.85,0.49-0.67 and 1.82-2.19 mg·mL-1.The contents of 7 components in tested sample from different manufacturers were closed.Conclusion The method has shown good sensitivity,accuracy,and repeatability.The study can provide reference and data support for the quality control and subsequent research of Qingkailing Oral Liquid.

Objective To investigate the expression of peripheral programmed death (PD)-1hiCXCR5-CD4+T cells and its clinical significance in systemic lupus erythematosus (SLE). Methods Peripheral blood PD-1hiCXCR5-CD4+ T cells from 21 SLE patients and 16 healthy controls were examined by flow cytometry. The levels of serum anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies were determined using immunoradiometric as-say. Data were analyzed with t test and Pearson's correlation test. Results The per-centages of PD-1hiCXCR5- cells within CD4+ T cell were significantly higher in SLE patients [(2.1 ±2.0)％] compared to normal controls [(0.3±0.3)％] (t=2.959, P<0.01). The percentages of PD-1hiCXCR5-cells within CD4+T cells in moderate to severe active SLE patients (3.0 ±2.0)％ was significantly increased compared to patients with mild or inactive (1.0±1.4)％(t=2.574, P<0.05) and normal controls (0.3±0.3)％ (t=5.149, P<0.01). The percentages of PD-1hiCXCR5- cells within CD4+ T cells from SLE patients were positively related with systemic lupus erythematosus disease activity index (SLEDAI) (r=0.475, P=0.0297). SLE patients in serum anti-dsDNA antibodies positive group (2.7±2.1)％displayed a higher percentage of PD-1hiCXCR5-cells within CD4+T cells than patients in serum anti-dsDNA antibodies negative group (0.6 ±0.5)％ (t=2.303, P<0.05). The percentages of PD-1hiCXCR5-cells within CD4+T cells from SLE patients were positively correlated with anti-dsDNA antibody titers. Conclusion The percentages of PD-1hiCXCR5- cells within CD4+ T cells from SLE patients are increased and are positively correlated with SLEDAI and anti-dsDNA antibody levels. Increased percentage of PD-1hiCXCR5-cells within CD4+T cells might play an important role in the pathogenesis of SLE.