BACKGROUND:Molars are characterized by multi-root, multi-root canal, multi-directional, different geometric shape and distribution. Single-root canal teeth post-core theory was used to guide molar repair in the clinic. It is easy to cause root canal perforation or vertical fracture due to excessive post preparation. Therefore, it is necessary to make further study and investigation in the design of fiber post-resin core for repairing molars. OBJECTIVE: To evaluate the clinical therapeutic effects of fiber post-resin core with different numbers of posts in the repair of molar residual roots and crowns. METHODS: A total of 54 human molar residual roots and crowns with sound root canal filing in 48 patients were selected and restored with fiber post of different numbers and resin core as wel as complete coronal restoration. There were 17 cases (20 samples) restored with single fiber post core, 16 cases (18 samples) restored with double fiber post cores, and 15 cases (16 samples) restored with three fiber post cores. They were folowed up for 24 months and the repair results were compared. RESULTS AND CONCLUSION:After 24 months of folow-up, the success rates were 85%, 94% and 94% in the single fiber post, double fiber post and three fiber post groups, respectively, and no significant difference was detected among the three groups. Five failures were observed among 54 teeth: three cases of fiber post shedding in the single fiber post group, one case of fiber post shedding in the double fiber post group, and one case of fiberpost shedding in the three fiber post group, and no root fracture occurred. Three kinds of fiber post-resin cores for repairing molar residual roots and crowns can get a better short-term clinical result. The repair effects were not different because of the different numbers of fiber posts.

Protein tyrosine phosphatase (PTP) 1B is a potential target for the treatment of diabetes and obesity. Phosphotyrosine (pTyr) is the substrate for PTP1B dephosphorylation. Malonic acid moiety was used herein as a mimic of the phosphate group in pTyr, and novel malonic acid derivatives 1-7 were designed, synthesized and evaluated as PTP1B inhibitors. Results from enzymatic assays indicated that compounds 3 and 4 exhibited potent inhibition against human recombinant PTP1B with IC50 values of 7.66 and 1.88 micromol x L(-1), respectively.

Based on public information on projects of medical and health technology development program in Shandong province,this article analyses the project approvals in Shandong medicine and health technology development program from 2005 to 2009.Characteristics in terms of project category,funding amount,host institution,subject distribution,etc.are presented,so as to provide reference for the relevant policy makers.

Background and purpose:Cancer of unknown primary (CUP) represents approximately 5%~10%of malignant neoplasms. For CUP patients, identiifcation of tumor origin allows for more speciifc therapeutic regimens and improves outcomes.Methods:By retrieving the gene expression data from ArrayExpress and Gene Expression Omnibus data repositories, we established a comprehensive gene expression database of 5 800 tumor samples encom-passing 22 main tumor types. The support vector machine-recursive feature elimination algorithm was used for feature selection and classiifcation modelling. We further optimized the RNA isolation and real-time quantitative polymerase chain reaction (RTQ-PCR) methods for candidate gene expression proifling and applied the RTQ-PCR assays to a set of formalin-fixed, paraffin-embedded tumor samples.Results:Based on the pan-cancer transcriptome database, we identiifed a list of 96-tumor speciifc genes, including common tumor markers, such as cadherin 1 (CDH1), kallikrein-re-lated peptidase 3 (KLK3), and epidermal growth factor receptor (EGFR). Furthermore, we successfully translated the microarray-based gene expression signature to the RTQ-PCR assays, which allowed an overall success rate of 88.4% (95%CI: 83.2%-92.4%) in classifying 22 different tumor types of 206 formalin-fixed, paraffin-embedded samples. Conclusion:The 96-gene RTQ-PCR assay represents a useful tool for accurately identifying tumor origins. The assay uses RTQ-PCR and routine formalin-ifxed, paraffn-embedded samples, making it suitable for rapid clinical adoption.

OBJECTIVE To investigate the antimicrobial susceptibility of Streptococcus pneumoniae isolated in Yantai and their mechanisms of resistance to macrolides.METHODS Antimicrobial susceptibility of S.pneumoniae was determined by agar dilution method.Phenotypes of macrolide-resistant S.pneumoniae were determined using double disk test with erythromycin and clindamycin disks.ermB And mefE genes were amplified by PCR.RESULTS Among 42 strains of S.pneumoniae,65.0% were intermediate to and no strain was resistant to penicillin.The resistance rates to erythromycin and clindamycin were 93.0%,respectively.Of 41 erythromycin resistantstrains,93.0% were constitutive resistant.ermB Was detected in 40 strains and mefE in 1 strain,both ermB and mefE genes were found in 9 strains.CONCLUSIONS The resistance rate of S.pneumoniae to penicillin is high in Yantai area,the resistance rates to erythromycin and clindamycin are very high.Target modification by ermB methylase is the predominant mechanism in macrolide-resistant S.pneumoniae in Yantai.

Aim To investigate the effects of high glu-cose on cholesterol metabolism in renal tubular cells and the intervention of the anthocyanins. Methods HK-2 cells were grown in the DMEM medium supple-mented with 10% FBS and were divided into 5 groups:normal glucose group, high glucose group, mannitol group, C3G group and Cy group. Effect of anthocya-nins on cell viability was detected with MTT, and cho-lesterol accumulation was detected with Amplex Red Cholesterol Assay kit and Filipin staining. Expression of ABCA1 was detected with RT-qPCR and Western Blot. Results In compared with control groups, HG significantly promoted cholesterol mass inside the cell and decreased the cholesterol concentration in the me-dium after treatment for 24 h or 48 h. The levels of mRNA and protein of ABCA1 were detected with RT-qPCR and Western blot, and both were decreased in the presence of HG. Whereas treatment with C3G and Cy markedly attenuated HG-induced cholesterol mass inside the cell by up-regulating the expression of AB-CA1. Conclusions High glucose can reduce the ex-pressions of the ABCA1, and then decrease cholesterol efflux and increase the cholesterol accumulation in HK-2 cells. Anthocyanins can decrease cholesterol accu-mulation by up-regulating the expression of ABCA1.

Objective To study the clinical significance of ST-T changes in electrocardio-gram to diagnose coronary heart disease in hypertensive patients.Methods One hundred and twelve patients with hypertension accompanied by chest pain in our hospital were divided into ST-T group with dynamic changes with 46 patients and ST-T group without dynamic changes with 66 patients according to ST-T changes.All patients were given electrocardiogram monitoring and coronary an-giography,and the relationship between the results of coronary angiography and ST-T dynamic changes was analyzed in both groups.Results There were 36 patients (32.1%)of positive coro-nary artery and 10 patients (8.9%)of negative coronary artery in ST-T group with dynamic changes,whereas there were 18 patients (16.1%)of positive coronary artery and 48 patients (42. 9%)of negative coronary artery in ST-T group without dynamic changes.The positive rate of coro-nary artery was evidently higher in ST-T group with dynamic changes than that in ST-T group without dynamic changes,and the difference was statistical significant.Conclusion ST-T dynamic changes in electrocardiogram of hypertensive patients has a certain correlation with the diagnosis of coronary heart disease,which can be considered as one of the references to diagnose the coronary heart disease.

Objective To study the clinical significance of ST-T changes in electrocardio-gram to diagnose coronary heart disease in hypertensive patients.Methods One hundred and twelve patients with hypertension accompanied by chest pain in our hospital were divided into ST-T group with dynamic changes with 46 patients and ST-T group without dynamic changes with 66 patients according to ST-T changes.All patients were given electrocardiogram monitoring and coronary an-giography,and the relationship between the results of coronary angiography and ST-T dynamic changes was analyzed in both groups.Results There were 36 patients (32.1%)of positive coro-nary artery and 10 patients (8.9%)of negative coronary artery in ST-T group with dynamic changes,whereas there were 18 patients (16.1%)of positive coronary artery and 48 patients (42. 9%)of negative coronary artery in ST-T group without dynamic changes.The positive rate of coro-nary artery was evidently higher in ST-T group with dynamic changes than that in ST-T group without dynamic changes,and the difference was statistical significant.Conclusion ST-T dynamic changes in electrocardiogram of hypertensive patients has a certain correlation with the diagnosis of coronary heart disease,which can be considered as one of the references to diagnose the coronary heart disease.

Objective To investigate the antimicrobial resistance profile of the clinical isolates collected from selected hospitals across China. Methods Twenty-nine general hospitals and five children's hospitals were involved in this program. Antimicrobial susceptibility testing was carried out according to a unified protocol using Kirby-Bauer method or automated systems. Results were interpreted according to CLSI 2017 breakpoints. Results A total of 190 610 clinical isolates were collected from January to December 2017, of which gram negative organisms accounted for 70.8％ (134 951/190 610) and gram positive cocci 29.2％ (55 649/190 610). The prevalence of methicillin-resistant strains was 35.3％ in S. aureus (MRSA) and 80.3％ in coagulase negative Staphylococcus (MRCNS) on average. MR strains showed much higher resistance rates to most of the other antimicrobial agents than MS strains. However, 91.6％ of MRSA strains were still susceptible to trimethoprim-sulfamethoxazole, while 86.2％ of MRCNS strains were susceptible to rifampin. No staphylococcal strains were found resistant to vancomycin. E. faecalis strains showed much lower resistance rates to most of the drugs tested (except chloramphenicol) than E. faecium. Vancomycin-resistant Enterococcus (VRE) was identified in both E. faecalis and E. faecium. The identified VRE strains were mainly vanA, vanB or vanM type based on phenotype or genotype. The proportion of PSSP or PRSP strains in the non-meningitis S.pneumoniae strains isolated from children decreased but the proportion of PISP strains increased when compared to the data of 2016. Enterobacteriaceae strains were still highly susceptible to carbapenems. Overall, less than 10％ of these strains (excluding Klebsiella spp.) were resistant to carbapenems. The prevalence of imipenem-resistant K. pneumoniae increased from 3.0％ in 2005 to 20.9％ in 2017, and meropenem-resistant K. pneumoniae increased from 2.9％ in 2005 to 24.0％ in 2017, more than 8-fold increase. About 66.7％ and 69.3％ of Acinetobacter (A. baumannii accounts for 91.5％) strains were resistant to imipenem and meropenem, respectively. Compared with the data of year 2016, P. aeruginosa strains showed decreasing resistance rate to carbapenems. Conclusions Bacterial resistance is still on the rise. It is necessary to strengthen hospital infection control and stewardship of antimicrobial agents. The communication between laboratorians and clinicians should be further improved in addition to surveillance of bacterial resistance.

Objective To investigate the distribution and antimicrobial resistance profiles of the common pathogens isolated from urine from 2015 to 2021 in the CHINET Antimicrobial Resistance Surveillance Program.Methods The bacterial strains were isolated from urine and identified routinely in 51 hospitals across China in the CHINET Antimicrobial Resistance Surveillance Program from 2015 to 2021.Antimicrobial susceptibility was determined by Kirby-Bauer method,automatic microbiological analysis system and E-test according to the unified protocol.Results A total of 261 893 nonduplicate strains were isolated from urine specimen from 2015 to 2021,of which gram-positive bacteria accounted for 23.8％(62 219/261 893),and gram-negative bacteria 76.2％(199 674/261 893).The most common species were E.coli(46.7％),E.faecium(10.4％),K.pneumoniae(9.8％),E.faecalis(8.7％),P.mirabilis(3.5％),P.aeruginosa(3.4％),SS.agalactiae(2.6％),and E.cloacae(2.1％).The strains were more frequently isolated from inpatients versus outpatients and emergency patients,from females versus males,and from adults versus children.The prevalence of ESBLs-producing strains in E.coli,K.pneumoniae and P.mirabilis was 53.2％,52.8％and 37.0％,respectively.The prevalence of carbapenem-resistant strains in E.coli,K.pneumoniae,P.aeruginosa and A.baumannii was 1.7％,18.5％,16.4％,and 40.3％,respectively.Lower than 10％of the E.faecalis isolates were resistant to ampicillin,nitrofurantoin,linezolid,vancomycin,teicoplanin and fosfomycin.More than 90％of the E.faecium isolates were ressitant to ampicillin,levofloxacin and erythromycin.The percentage of strains resistant to vancomycin,linezolid or teicoplanin was＜2％.The E.coli,K.pneumoniae,P.aeruginosa and A.baumannii strains isolated from ICU inpatients showed significantly higher resistance rates than the corresponding strains isolated from outpatients and non-ICU inpatients.Conclusions E.coli,Enterococcus and K.pneumoniae are the most common pathogens in urinary tract infection.The bacterial species and antimicrobial resistance of urinary isolates vary with different populations.More attention should be paid to antimicrobial resistance surveillance and reduce the irrational use of antimicrobial agents.