Purpose: To evaluate the effect of a gymnastics program for eight weeks to maintain better body composition and decrease fatigue among factory workers. Methods: Data were collected in 54 female workers in their 50s and 60s in a factory located in the S city of the K region between April and July in 2019. The subjects were divided into two groups: 1) experimental group (n=27) received a 30 minute-gymnastics program three times per week, and 2) control group participated in lecture series about the principle and effect of the gymnastics program. Body composition and fatigue levels were measured before and after the intervention and analyzed using the SPSS 23.0 program. To evaluate the differences in demographic and clinical characteristics between the experimental and control groups, the chi-squared test and the t-test were used. Repeated measures two-way ANOVA was performed to evaluate the change between outcomes of the pre-and post-investigation. Pairwise comparisons were used to assess the differences between the groups and the time. Results: There are statistically significant differences in body fat mass (p<.001), body fat percentage (p<.001), basal metabolic rate (p<.001), and body mass index (p<.022) in the experimental group compared to the control group. In particular, there is a significant interaction between the groups and the time in body fat mass (F=6.308, p=.015), basal metabolic rate (F=11.397, p=.001), fatigue (F=6.933, p=.011). Conclusion Our findings suggest that using the gymnastics program helps women in their 50s and 60s who work in factories promote better body composition and reduce fatigue levels.