Aim To determine the function of nuclear factor-κB ( NF-κB ) in immunological liver injury of rat model and its effect on CYP2E1 expression, content and metabolic activity. Methods The immunological liver injury rat model was prepared by injection of Ba-cillus Calmette Guérin ( BCG,125 mg · kg-1 ) for 14 days. The hepatic tissue injury was revealed by hema-toxylin and eosin ( HE ) method and serum concentra-tion of alanine aminotransferase( ALT) , aspartate ami-notransferase ( AST ) respectively. CYP450 total con-tent in hepatic homogenate was determined by spectro-photography. The expression of CYP2E1 protein was detected by Western blot analysis. The enzyme kinetics of CYP2 E1 probe drug chlorzoxazone was evaluated by high-performance liquid chromatography ( HPLC ) as-say. Results The results showed that BCG-pretreat-ment ( 125 mg · kg-1 ) significantly increased the weight of liver and spleen, serum levels of ALT and AST(P<0. 01) , and decreased CYP2E1 expression, content and metabolic activity ( P <0. 05 ) . Adminis-tration of ammonium pyrrolidine dithiocarbamate (PDTC) (50, 100, 200 mg·kg-1) reversed the a-bove hepatic injury stimulated by BCG in vivo. Moreo-ver, PDTC dose-dependently inhibited the down regu-lation of CYP2 E1 ( P<0. 05 ) . Conclusion Passiva-tion of NF-κB can inhibit the down regulation of CYP2 E1 in liver tissue of immunological liver injury rats;NF-κB may be involved in CYP2 E1 down-regula-tion.

Objective:To investigate the status of social alienation in maintenance hemodialysis (MHD) patients, and analyze its correlation with self disclosure and social support.Methods::From May 2019 to March 2021, 156 MHD patients admitted to Nanyang Central Hospital were selected as the research objects by using convenient sampling method. A cross-sectional survey was conducted by using general information questionnaire, general alienation scale, pain disclosure index scale and social support scale.Results:A total of 176 questionnaires were issued, with an effective recovery rate of 88.64%(156/176). The social alienation score of 156 patients with MHD was (41.57±4.89) points, which was at the upper-middle level and negatively correlated with the total scores of the Distress Disclosure Index and the Perceived Social Support Scale ( r=-0.526, -0.284, all P<0.01). The results of multiple linear regression analysis showed that age, educational level, and per capita monthly household income were the main factors affecting social alienation ( β=0.199, -0.361, -0.290, all P<0.01). After controlling for the above factors, self disclosure and social support independently explained the variance of social alienation in MHD patients increased by 20.2% ( β=-0.440, -0.200, all P<0.01). Conclusions:MHD patients have a high sense of social alienation. Medical staff should pay attention to patients who are older, less educated, and have low family income. Self disclosure and social support are modifiable variables, so medical staff can reduce their social alienation and improve the quality of life by training patients on self disclosure and improving the level of social support.

Objective To explore the influence of AKT inhibitors on tumor infiltrating T lymphocytes (TIL)in patients with liver metastasis of colorectal cancer.Methods The tumor tissues from the patients with liv-er metastasis of colorectal cancer in Department of General Surgery,The Affiliated Cancer Hospital of Zhengzhou University from January 2016 to December 2016 were collected.TIL and tumor cells were isolated by percoll densi-ty gradient centrifugation. The profiling of AKT inhibitors on TIL were analyzed by flow cytometry. Results AKT inhibition enhances the expansion of TIL with memory cell without affects its proliferation,also the cells obtained under AKT inhibitor with IL-2 showed higher frequency of IFN-γproducing cells than IL-2. Conclusion Add AKT inhibitors in TIL cultivation system can strengthen the proliferation of central memory T cells,and does not affect the number of CD8+T cells.This might be developed for cell-based immunotherapy of cancer.