Objective To estabhsh a set of consummate evaluation methods of animal models which are with syndromes of Traditional Chinese Medicine.Methods By means of Streptococcus pneumoniae-induced pyretic pulmonary syndrome model for the entry point,in the base of the conventional evaluation methods,further attempt were made through metabonomies,through analyzing metabolic fingerprint data of rats'urine in the control group together with the model group and metabolome of rats'urine in model group at different times,in order to approach the evaluation methods of animal models.Results After rats were given Streptococctts pneumoniae through nose,compared with the control group,both the body temperature variance and lencocyte count in model group were statistically significant with P <0.01,pathological changes of lung was obvious;According to the metabolic results,metabolic profiles of rats'urine in model group changed and metabolome diverged obviously in scores plot,the research results of metabonomics were coincide with the results of macroscopy physical sign and pathological biochemistry of Pyrefic pulmonary syndrome.Conclusion Metabonomics can be used to evaluate the studies of animal models with syndromes of traditional Chinese medieine.

OBJECTIVEMetabonomics researches of Scutellariae Radix interfering pyretic pulmonary syndrome had been done, to determine the specific biomarkers of pyretic pulmonary syndrome, and to approach the mechanism that Scutellariae Radix interfered the variation of these biomarkers.

METHODMetabonomics technique, UPLC-Q-TOF/MS analytical means and PCA statistical methods were utilized to investigate the trajectory change and inter-relationship of urinary metabolome of rats treated differently.

RESULTSix specific biomarkers were determined which could represent Streptococcus pneumoniae-induced pyretic pulmonary syndrome in rats. Scutellariae Radix could significantly adjust the ascended biomarkers to the normal level. Meanwhile two of these biomarkers were identified as Delta-12-prostaglandin J2 and indoxyl sulfate.

CONCLUSIONThere was a good therapeutic function of Scutellariae Radix on pyretic pulmonary syndrome, which was elucidated on the metabolic aspects. There was also a correlationship between the mechanism of Scutellariae Radix curing pyretic pulmonary syndrome and the six specific biomarkers.

Animals ; Drugs, Chinese Herbal ; therapeutic use ; Lung Diseases ; drug therapy ; metabolism ; Male ; Pneumococcal Infections ; drug therapy ; metabolism ; Rats ; Rats, Wistar ; Scutellaria baicalensis ; chemistry