Objective To explore the correlative factors of mental disorder in patients with head injury. Methods 255 subjects sustained with head injury and survived from traffic accident in Chengdu were assessed with Structured Clinical Interview for DSM-Ⅳ axis Ⅰ disorders (SCID-I/P) , Personality Disorder Questionaire for CCMD-2-R(PDQC), Structured Clinical Interview for CCMD-2-R(SCICP), WAIS-RC, WMS, brain electric activity mapping (BEAM) after completion of clinical management. Based on the diagnostic criteria of organic mental disorder in CCMD-3, mental disorders in 255 subjects were diagnosed. Results The incidence of mental disorders in the patients with head injury was 72.5% (185/255). Mental disorders in patients with head injury were correlated with educational level, type and severity of the head injury, or injuries involving the left frontal lobe injury, the left temporal lobe or multiple lobes and BEAM abnormality. Conclusion The incidence of mental disorder is high in the patients with head injury. Educational level, type and severity of the head injury, the location of the injured brain, injury involving multiple lobes of the brain may affect morbidity of mental disorders in patients with head injury.

the living area, and the PAHs with four rings to five rings in PM10 were the dominant PAHs, which proves that the sources of PM10 in autumn have a certain similarity in Huainan city.

The respective sense and antisense oligodeoxynucleotides and their phosphorothiods, targeting at the bcr/abl, were the hallmark gene of Chronic Myelogenous Leukemia( CML) and c-myb correlates with CML. K562 cells, derived initially from a patient of CML blast crisis, were incubated with bcr/abl antisense oligodeoxynucleotides asODN or c-myb asODN or with both asODN in combinalion. All kinds of asODN could not only significantly inhibit K562 cells survival with the highest inhibitory rate of 64.7%?3.2%, but also dramatically inhibit DNA synthesis and the highest inhibitory rate was 85. 8 %?4.1%, decrease bcr/abl mRNA level almost completely and induce apoptosis significantly. The inhibition effect of antisense oligodeoxynucleotides phosphorothiodes s-asODN was stronger than that of unmodified asODN. The inhibition of the combination of bcr/abl and c-myb asODN was stronger than that of anyone alnoe. All the inhibitions exhibited in sequence-and time-dependent manner. We concluded that bcr/abl in pathogenesis of CML not only increased CML cells proliferation rate, but also decreased CML cells apoptosis rate. c-myb may participate in CML mainly by regulating bcr/abl. Trie results indicated that the modified asODN and multiple asODN targeting several oncogenes may advance to antisense gene therapy.

Objective To establish a method for the determination of berberine hydrochloride in Siji Sanhuang capsules(SSC).Methods The RP-HPLC method was performed on Agilent Hypersil ODS column(4.6 mm?150 mm,5 ?m).Acetonitrile and 0.05 mol/L NaH2PO4 water(adjust pH to 3 with H3PO4)were used as the mobile phase(30 ∶70),detection wavelength was at 270 nm,the flow rate was 1.0 mL/min and the column temperature was at 25 ℃.Results In the range of 0.094 to 0.470 ?g,berberine hydrochloride had a good linearity with chromatograph peaks area(r=0.998 3),the average recovery ratio was 98.5 %(n=5),and RSD=2.3 %.Conclusion This method is simple,effective and with a higher sensibility and stability.The method can be used to control the quality of SSC effectively.

Objective To investigate the efficacy and safety of fludarabine plus mitoxantrone combination chemotherapy for malignant lymphoma compared with CHOP regimen.Methods Sixty-six patients with malignant lymphoma were divided into fludarabine group and CHOP group.The efficacy and toxicities of these two regimens were analyzed and compared between the two groups.Results The overall response(OR)was 81.5% in fludarabine group,while was 48.7% in CHOP;for the patients in late-stage,with B cell lymphoma,with T cell lymphoma and with high serum LDH level,the OR in fludarabine group was higher than in CHOP group obstructively,being 62.5% and 35.7%.Conclusion Fludarabine combination chemotherapy,as a first line regimen in treating first-treated lymphoma,recrudescent lymphoma,intractable lymphoma and lymphoma in late stage,possesses higher efficacy,safety and tolerance than CHOP regimen.

Objective To investigate the clinical efficacy of methotrexate and mifepristone in treatment of ectopic pregnancy. Methods 58 cases with ectopic pregnancy admitted in the Second People's Hospital in Putuo district of Zhoushan city from February 2010 to February 2012 were randomly divided into observation group and control group,each had 29 cases. Control group were received methotrexate 1 mg/kg or 50 mg/m2 once a day by intramuscular injection,observation group were given both methotrexate and mifepristone treatment,the mifepristone was given one piece a day and each piece is 75 mg. the treatment success,b-HCG returned to normal time,normal pregnancy and the occurrence of complications were compared between two groups. Results The treatment success and normal pregnancy rate in observation group were significantly higher than control group(P<0.05),b-HCG returned to normal time was significantly shorter than control group (P<0.05 ). There were no obvious adverse reactions in both two groups, observation group had one light nausea,and control group had one light nausea and one case with alanine aminotransferase (ALT)slightly increased phenomenon,those patients were all back to normal on their own without treatment,the difference of adverse reaction rate between two groups was not significant. Conclusions Methotrexate and mifepristone has good efficacy and are safe and reliable in,treatment of ectopic pregnancy.

AIM: To evaluate the antihistamine effects of domestic azelastine hydrochloride. METHODS: Histamine increased skin vascular permeability and induced shock model in guinea pigs. In isolated guinea pig ileal rings, the contraction of smooth muscle induced by histamine was determined. RESULTS: Pretreament with azelastine hydrochloride( 0.05, 0.15,and 0.45 mg?kg -1,ig) significantly inhibited the increase in skin vascular permeability induced by histamine in a dose-dependent manner in guinea pigs. Pretreament with azelastine hydrochloride( 0.05, 0.1,and 0.2 mg?kg -1,ig)produced a significant improvement of shock, as shown by a decrease in reactivity degree, mortality and a prolongation of latency. In isolated guinea pig ileal rings, azelastine hydrochloride (10 -8,3?10 -8,10 -7,3? 10 -7 mol?L -1)significantly inhibited contraction of smooth muscle induced by histamine in a concentration-dependent manner and caused a parallel right shift of the dose-effect curve of histamine (pA 2= 8.55). CONCLUSION: Domestic azelastine hydrochloride shows significant the antihistamine effects.

Objective To study the intelligence and memory impairment of patients with craniocerebral injury during traffic accident and its correlated factors. Method Data of psychological tests, eletroencepa-lograph (EEG) and brain image information of 652 subjects with brain injury who survived from traffic accident in Chengdu from Jan. 1,1999 to Dec. 31,2001 were collected and analysed. Results The average intelligence quotient (IQ) of patients in patients group was 77.85?15.97. 27.9 % patients's IQ was lower than 70. 51.9% patients's memory quotient (MQ) was lower than 51. The intelligence impairment was correlated with education level, left temporal lobe injury, three or more lobes of the brain injuried and EEG abnormality. Memory impairment was correlated with education level, interval between injury and e-valuation, brain stem injury, left temporal lobe injury, and EEG abnormality. Conclusion Impairment of intelligence and memory in different degrees were observed in most patients with traumatic brain injury during traffic accident. Intelligence and memory impairment were correlated with left temporal lobe injury, EEG abnormality, brain stem injury and education level.

Objective To assess the safety and efficacy of an olive oil-based lipid emulsion for parenteral nutrition in patients after hepatectomy.Methods Thirty-one postoperative patients with elective hepateetomy were randomized to receive isonitrogenous,isocalorie parenteral nutrition over 6 days after liver lobectomy(48-72hours)with either olive oil-based lipid emulsion(study group,n=15)or standard soybean oil emulsion(control group,n=16).The liver function and plasma protoins were assessed using peripheral venous blood collected before surgery,one day after surgery,and 7 days after surgery.The safety profiles of emulsion supports and postoperative rehabilitation were also assessed.Results The preoperative serum levels of total bilirubin,direct bilirubin,alanine amiotransferase,aspartate aminotransferase,alkaline phosphatase,total protein,albumin,and prealbumin were comparable between the two groups(all P>0.05).Although the Postoperative safety profile and liver function were not significantly different between two groups(all P>0.05),plasma total proteins,albumin,and prealbumin returned to the normal levels significantly faster in the study group than in control group[(57.57±9.84)g/L vs.(47.76±6.53)g/L,P=0.000;(31.29±3.11)g/L vs.(26.34±4.87)g/L,P=0.000;(0.188±0.059)g/L vs.(0.103±0.037)g/L,P=0.000]on the 7th Postoperative day,and the Postoperative hospital stay was also significantly shorter in the study group[(13.1±1.2)d vs.(15.2±1.1)d,P=0.041].The incidence of postoperative complications in study group and control group was 26.7%and 31.3%.respectively.Conclusions Treatment with the new olive oil-based lipid emulsion is weU tolerated in hepatectomy patients.It can speed up plasma proteins recovery and may shorten postoperative hospital stay,although it does not remarkably decrease the incidence of postoperative complications.

Objective To investigate the application effect of the new trinity teaching mode of clinical practice,which combines bedside teaching,simulation teaching and web-based teaching to-gether. Methods Totally 375 students of clinical medicine,enrolled into Guangzhou Medical Univer-sity in 2006,were randomly divided into department I of clinical medicine (control group,188 stu-dents)and department II of clinical medicine (experimental group,187 students). The students of these two groups were taught respectively with the conventional bedside teaching mode and the trinity teaching mode. Then at the end of clinical practice,assessment results of comprehensive theory and clinical skill in the graduation exam were compared by t test (significance level α=0.05)using SPSS 17.0 software and the questionnaire results of students in experimental group were analyzed. Results There were statistical differences in comprehensive theory and clinical skill assessment scores between two groups(P<0.05). The questionnaire survey showed an approval rate of 92.73%(153/165)for trini-ty teaching mode. Conclusions Facing the shortage of clinical teaching resources,the trinity teach-ing mode is better than the simple bedside teaching mode. The trinity teaching mode can alleviate the pressure brought by the lack of clinical teaching resources at present,create more learning and prac-ticing opportunities for students,and improve the effects of clinical practice teaching.