The overexpression of EGFR and the typeⅠcAMPdependent protein kinase(PKAⅠ) has been found in most cancer tissue and tumour cells.The blockade of EGFR activation by using anti-EGFR monoclonal antibodies(MAbs) and inhibition of PKAⅠ expression by specific pharmacological agents such as the selective cAMP analogue 8-Cl-cAMP has been proposed as a potential anticancer therapy.We have shown that an interaction between EGFR and PKAⅠ occurs through direct binding of the RⅠsubunit to the Grb2 adaptor protein.We have demonstrated that the functional interaction between the EGFR and the PKAⅠ pathways could have potential therapeutic implications.In fact,the combined interference with both EGFR and PKAⅠ with specific pharmacological agents,has a cooperative antiproliferative effect on human cancer cell lines in vitro and in vivo.Studies on the antitumor activity of this combination are under human clinical trial evaluation.

Objective:A wearable ECG monitoring system is designed. Methods:By biofeedback technology and biofeedback training, a wearable ECG monitoring system with functions of relaxation therapies and action therapies comes true. By technology of RFID-based internet of things, wireless data transmission is obtained. And with MATLAB, the analyses of ECG data at home are attained simply. Results: The system realizes the wearable ECG monitoring. It can promote sufferers’ subjective initiative effectively to make his spontaneous physiologic and mental regulations, and the prevention or healing will be achieved. Conclusion:The wearable ECG monitoring system by technologies of biofeedback and RFID-based internet of things, with strong practicability, can be widely applied.

Objective To understand true feelings of patients with malignant tumor after implementing the protective medical treatment.In order to provide more humanized service for clinical nursing and to improve the job satisfaction of nurses.Methods The phenomenological methods of qualitative research was applied to carry out interviews with 12 patients to collected data.Then the data were analyzed.Results After reading,analysis,reflection,classification and refining the theme,we drew the following themes:(1)Before knowing diagnosis:half-believe and anxiety.(2) At the time of knowing diagnosis:luck,avoidance,acceptance and facing; (3) After knowing diagnosis:thirsting for information,low self-worth,improving self motivation.Conclusions We should not insist on hiding malignant tumor patients.Individualized and targeted measures should be taken to make patients cope with diagnosis better and improve the quality of their life.

Objective To investigate the indoors air formaldehyde pollution in the human anatomy laboratory and its effects on students health. Methods AHMT method was used to determine the concentration of formaldehyde in the indoor air and to observe the students’ signs and symptoms in the near month. Results The level of formaldehyde in the air of human anatomy laboratory was higher than that of the control and was higher than national standard limit. The prevalence rate of eye symptoms(51.98%), nasal symptoms(58.91%), throat symptoms(48.02%), nausea and vomiting, psychiatric symptoms(68.32%) and cold liability (60.89%) in the exposed group was higher than those in the control group(P

This study was aimed to explore the methodology regarding culture, proliferation and purification of adipose-derived stromal cells (ADSCs), and to study their biological characteristics. ADSCs were obtained using type I collagenase digestion method. Cell growth was observed, and cell viability were detected under different digestion period by MTT. The ADSCs were then identified and induced. The results showed that adherent cells digested by type I collagenase for 60 min had a strong proliferation capability. After the induction of different inducers these adherent cells could differentiate into nerve cells and fat cells. The best digestion period was proved to be of 60 minutes in the experiment. The results indicate that stem cells with multilineage differentiation ability could be separated from adipose tissue, namely ADSCs.
Adipocytes ; cytology ; Adipose Tissue ; cytology ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Neurons ; cytology ; Primary Cell Culture ; Stem Cells ; cytology ; Stromal Cells ; cytology

Objective:A remote monitoring system for ICU is designed.Methods: By technology of RFID-based internet of things, data processing software platform is designed. Combined with RFID tags, readers etc., ICU patients are effectively identified and identifying information transmission and computer management are complied. And then a remote monitoring system for ICU is achieved.Results: The system realizes the ICU remote monitoring and effective prevention of nosocomial infections, and meets the families of patients remote access needs; the system can integrate with HIS/CIS seamlessly to enhance the ICU clinical services integrated computerized management further.Conclusion: The remote monitoring system for ICU by technology of RFID-based internet of things, with strong practicability, can be widely applied.

Objective To investigate the effect of different dose of GM1 on inducing human adipose tissue-derived stromal cells (ADSCs) into neural cells. Methods Human ADSCs were isolated from human liposuction tissues and cultured in NIM with different dose of GM1, which was used to induce ADSCs into neural cells. The experiment was separated into six groups: control group (?-MEM), Group A (NIM), group B (NIM+50 ?mol/L GM1), group C (NIM+100 ?mol/L GM1), group D (NIM+200 ?mol/L GM1) and group E (NIM+400 ?mol/L GM1). Cells' growth and morphologic changes were observed under invert phase-contrast microscopy. The expressions of Nestin, NSE, MAP2 and GFAP were identified by immunocytochemistry methods.Results (1)ADSCs could differentiate into neural cells in NIM with different dose of GM1. The number of neural cells differentiated from ADSCs increased in a certain period, especially in group E at each time point (all P

Objective To investigate the correlation about the quantitative test result of 13C-urea breath test(13C-UBT) with the quantity of Helicobacter pylori( H.pylori) and the degree of gastric mucosal inflammation.Methods 13 C-UBT was tested in the children with upper gastrointestinal symptoms.Ninety-five cases of the children with positive result (DOBδ‰ ≥4.0) underwent endoscopy and gastroscopic biopsy.The correlation was analyzed between the result of the 13 C-UBT and the severity of gastric H.pylori infection and gastric mucosal inflammation.Results There were significant differences about DOB values between the group with severe gastric H.pylori infection and the group with mild to moderate infection ( P ＜ 0.05 ),the same result was observed between the group with severe gastric mucosal inflammation and the group with mild to moderate inflammation( P ＜ 0.05 ).There was certain correlation between DOB value and the amount of gastric H.pylori colonization and mucosal inflammation.Conclusion The quantitative test result of 13C-UBT could indicate the number of gastric H.pylori colonization,but lack a precise quantitative relationship between them.There was positive correlation between the DOB values and the degree of gastric inflammation.

Objective To study the relationship between extracranial carotid arerial stenosis and OCSP typing of acute cerebral infarction. Methods 188 patients with acute cerebral infarction were classified into TACI, PACI, POCI and LACI subtype by OCSP criteria. The lumen diameter, intima-media thickness, plaque, severity of ar-terystenosis of extraeranial carotid arteries in patients with acute cerebral infarction was studied by using HDI-5000 color Doppler ultrasound. Resnlts The prevalence of extracranial carotid arterial stenosis was higher in patients with TACI, POCI subtype than in patients with LACI subtype (X2 = 4.87, P < 0.05 ; X2 = 4.58, P < 0.05). The severity of extracranial carotid arterial stenosis were more prominent in patients with TACI,POCI subtype than in patients with LACI subtype(X2 =7.59,P <0.01 ;X2 =6. 96,P <0.01). Conclusion Extraeranial carotid arterial stenosis and its severity are related to TACI, POCI subtype of OCSP criteria in patients with acute cerebral infarction.

Objective To observe the degradation pattern of tubulin in the cardiac muscle and lung tissue of rats after death.Methods 24 young male Sprague-Dawley rats were divided into 6 groups averagely.After being killed by bleeding,the rats were kept in an artificial climate incubator;the experimental temperature was kept at 20℃ and humidity at 50%.Proteins in cardiac muscle and lung tissue were extracted and tubulin was then examined by Western blot.Thereafter,the semi-quantitative analysis of the Western blot images was performed.Results Tubulin can be detected in the normal cardiac muscle and lung tissue of rats.After 1d postmortem,the tubulin content in these tissues began to decline,and it might still be detected at 7d postmortem,but the tubulin content in lung decreased faster than that in cardiac muscle.A linear relationship between tubulin content in cardiac muscle and lung of rats and the postmortem interval was determined.The linear regression equations and coefficients for integral optic density in relation to PMI were y=-1726.1x+14083 with correlative coefficient r=0.9684 for cardiac muscle and y=-1439.89x+12041 with correlative coefficient r=0.9808 for lung,respectively.Conclusion The level of tubulin in cardiac muscle and lung tissue of rats varied with PMI and the time dependent degradation of this protein was potentially useful for the PMI estimation.