ObjectiveTo explore alterations of serum levels and clinical significance of corticotropin releasing hormone (CRH), thyrotropin releasing hormone (TRH) and gonadotropin releasing hormone (GnRH) in patients with fibromyalgia (FM). MethodsA total of 55 subjects participated in this study: 29 healthy volunteers and 26 patients with FM recruited from Department of Neurology, the First Affiliated Hospital of Anhui Medical University from June 2009 to October 2010.The depression rate was assessed by Hamilton Depression Rating Scale-17. ELISA was used for the detection of the serum levels of CRH, TRH and GnRH.Normal distribution quantitative data were described by the (-x) ± s and tested by independent sample t-test. Non-normal quantitative data were described by interquartile range and tested by independent Mann-Whitney.The diagnostic specificity and sensitivity of 3 kinds of hormones test were analyzed by receiver operator characteristic ( ROC ) curve, and the Spearman correlation was used for analysis of hormone levels and age, gender, tenderness, pain degree and depression severity.Results Compared with the control (70. 0(48.7,78.0) ng/L), the fibromyalgia patients had obviously increased CRH (271.9 (210.9,326.5) rg/L, x2 =6.408, P＜0. 01) , and significantly higher TRH ((82.7 ±6. 9 ) ng/L vs ( 87. 2 ± 6. 8 ) ng/L, t = 2. 560, P ＜ 0. 05, respectively) and GnRH ( ( 18. 2 ± 0. 9 ) ng/L vs ( 19. 9 ± 1.6)ng/L,t =5. 324, P ＜0. 01, respectively). The serum concentrations of the CRH, TRH and GnRH were positively correlated with pain intensity and numbers of tenderness respectively, and those of the CRH and GnRH were positively correlated with depressive degree either. The areas under the ROC curve in the CRH, TRH and GnRH, evaluating the sensitivity and specificity for diagnosis of fibromyalgia, were respectively 1. 000, 0. 684 and 0. 854. ConclusionsThe FM patients had an increased secretion of CRH,TRH and GnRH. CRH might serve as the adjunctive criteria for the diagnosis of FM.

Objective To explore the common influencing factors on the severity of Reinke's edema of the vo-cal cord and to analyze the factors of recurrence.Methods To analyze the relationships among the degrees and smoking,voice overuse,and laryngopharyngeal reflux for 85 patients with Reinke's edema of the vocal cord,and an-alyze the risk factors of recurrence.ResuIts 84.7% of the patients were male with the mean age of 51.35 years. There were statistical differences among smoking history of more than 25 years (P<0.001),laryngopharyngeal re-flux (P<0.01),and voice overuse (P<0.01).Follow ups lasted 1 year and 7 patients showed recurrence.ConcIu-sion Reinke's edema was related to long - term smoking and common in senior male patients.The degree of Reinke's edema was positive correlation with smoking,laryngopharyngeal reflux and voice overuse.The most impor-tant factor is smoking history of more than 25 years.The longer smoking history,the more severe Reinke's edema.

Objective:To experimental study the inhibiting effects of SBHL on the growth of cervical cancer cells and to investigate the mechanisms.Methods:The proliferation of ME180 cells was observed by MTT assasy; The changes of cells cycles and apoptosis of ME180 cells treated with SBHL were analyzed by agar gel electrophoresis and FCM assay;. The changes of the ultra structure of cells was observed by Transmission eleconmicrograph.Results:The proliferation of ME180 cells was inhibited after treating on SBHL for 24 h.The inhibiting effect increased granually following the concentration of SBHL(P

[Objective]To statistically analyze the citations of papers in "The Orthopedic Journal of China"published during 2004~2006.[Method]Using the biblio metric method to calculate the citation rate,number of citations per paper,types of citations,language-based citation number,Price index and self-citation rate.[Result]During 2004~2006,the average citation rate was 94.32% and the number of citations per paper was 6.31.The number of citations in English was significantly higher than that in Chinese or other foreign languages.Meanwhile,the citation rate of papers published in journals was markedly higher than that of those published in books and other materials.The Price index and self-citation rate were 71.48% and 8.74%,respectively.[Conclusion]"The Orthopedic Journal of China"is shown to be a hight professional academic journal in China,with good novelty citation,high level of authors' education and extensive social influence.

Clinical data of 12 patients with pernicious placenta previa admitted in our hospital from October 2015 to July 2016 were retrospectively reviewed.Bilateral internal ilia artery balloons were placed percutaneously before cesarean section.After cesarean delivery normal saline was applied to fill the balloon for temporary occlusion of bilateral iliac arteries,then placental separation and hysterorrhaphy were performed.The average volume of bleeding during cesarean section was 1 146 ml.There were no hysterectomy,pelvic organ necrosis,lower limb artery thromosis and other complications.The application of temporary internal iliac artery balloon occlusion can significantly reduce the intraoperative bleeding in cesarean section for patients with pernicious placenta previa.It provides a clear operational field for obstetricians to perform the placental separation and hysterorrhaphy,facilitating the uterine reservation.

Objective:To clone and express the Der f 7 recombinant protein from Dermatophagoides farinae and prepare the Der f 7 monoclonal antibody.Methods: The Der f 7 recombinant protein was expressed in prokaryotic expression system of pET28a(+)-Der f 7.BALB /c mice were immunized with the recombinant protein.Myeloma cells and spleen cells were fused,and hybridoma cells were screened by ELISA.Hybridoma cells were injected into the mice abdominal cavity to obtain ascites.It was purified by protein A agarose medium ascites,and then to dentified the titer and purity of the antibody.The specificity of the antibody was identified by Western blot.Results: Three hybridoma cells which stably secret recombinant Der f 7 monoclonal antibody were obtained.The monoclonal antibody had high purity,the titer was higher than 1∶243 000.Western blot showed that Der f 7 recombinant protein could be recognized well.Conclusion: We successfully obtained Der f 7 monoclonal antibody,which can be used for the quantification and localization of Der f 7 allergen and the diagnosis and treatment of allergic diseases.

AIM:To determine which species of snake venoms contained protein c activator among 9 species of Chinese snake venoms. METHODS: Anticoagulant activity was examined by activated partial thromboplastin time (APTT) assay，and amidolytic activity was measured with activated protein c (APC) specific chromogenic peptide substrate-chromozy APC. RESULTS: Among 9 species of Chinese snake venoms，Trimeresurus mucrosquamatus venom and Agkistrodon halys venom were not only able to generate amidolytic activity from purified human PC, but also prolonged APTT strongly even at such a concentration as 1.5 mg/L.CONCLUSION: Trimeresurus mucrosquamatus venom and Agkistrodon halys venom contain protein c activator which activating human plasma PC into APC.

Objectives: Hypertensive disorders complicating pregnancy(HDCP) has a long-term deleterious effect.This study aimed to investigate the changes of cognitive function in women with HDCP and explore their possible mechanism.Methods: Fifty-three patients with HDCP and 22 normal pregnant women were included in this study,their cognitive functions assessed 60-80 days after delivery by word learning and delayed recall test,symbol digit substitution test,animal category fluency test,block design,trail making test(Part A) and digit span test.The results of the cognitive tests were compared between the HDCP and normal control groups.Results: Cognitive performances of the severe preeclampsia patients differed significantly from those of the normal individuals on the tests of word learning(P

AIM:To determine which species of snake venoms contained protein c activator among 9 species of Chinese snake venoms. METHODS: Anticoagulant activity was examined by activated partial thromboplastin time (APTT) assay,and amidolytic activity was measured with activated protein c (APC) specific chromogenic peptide substrate-chromozy APC. RESULTS: Among 9 species of Chinese snake venoms,Trimeresurus mucrosquamatus venom and Agkistrodon halys venom were not only able to generate amidolytic activity from purified human PC, but also prolonged APTT strongly even at such a concentration as 1.5 mg/L.CONCLUSION: Trimeresurus mucrosquamatus venom and Agkistrodon halys venom contain protein c activator which activating human plasma PC into APC.

Objective To establish the breastfeeding evaluation indicator system for preterm infants and to provide theoretical basis for breastfeeding implementation and evaluation.Methods Based on literature review,clinical on-spot investigation and experts consultation,the evaluation system frame was initially formed.With specialists meeting method and Delphi study,the evaluation indicator system for preterm infants was established.Results The breastfeeding evaluation indicator system for preterm infants comprised 3 levels,with 3 indicators in level 1,7 indicators in level 2 and 18 indicators in level 3.Conclusions It is emphasized that the purpose for breastfeeding evaluation of preterm infants is to continuously improve the breastfeeding quality for preterm infants.