The vectors play an important role in gene therapy by transporting the therapy genes into target tumor cells. At present there are mainly two kinds of gene vectors, including biological and non-biological vectors. Biological vectors, including virus, bacteria and stem cells, have good target activity and can efficiently delivery the genes into target tumor cells, but have potential genetic hazards.However, non-biological vectors, including liposomes and nanoparticles, can be simplely prepared with high genetic safety, but they can not transport the genes efficiently. Therefore, establishing safe and effective malignant glioma gene treatment system and finding gene vectors with good target activity become the research focus.

Purpose:To study the etiological role of Val384Asp in hMLH1 gene in gastric and esophageal cancer.Methods:Genomic DNA were extracted from peripheral blood and were subjected to PCR SSCP and DNA sequencing to screen Val384Asp in hMLH1 gene in 79 gastric and 76 esophageal cancer patients, and in 79 and 76 first degree relatives of gastric and esophageal cancer patients respectively, and in 100 healthy persons. Results:There is a significant difference in the frequency of Val384Asp between gastric cancer patients with family history and healthy controls ( P 0.05). Conclusions:The alleles frequency of Val384Asp in Chinese is 3%. It may partly play an etiological role in some of the stomach cancer patients.

OBJECTIVE:To establish the technical system that is suitable for protein extracting in Angelicae sinensis seed and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE),and provide technical support for detecting the protein quality and variety purity. METHODS:Using protein content and number of electrophoretic bands as indexes,8 methods,includ-ing voncentrated gel method,salt-soluble protein method,electrode buffer method,dimercaptosyl alcohol (DTT) method,urea method,mercaptoethanol method,trimethylolaminomethane (Tris) method,and acetone precipitation method,were conducted to extract the protein in A. sinensis seed and screen the optimal extraction method. Then based on optimal extraction method,effects of different materials-lipid ratios,sample dilution times(sample volume)and separate gel concentration on SDS-PAGE were investi-gated. RESULTS:Mercaptoethanol method extracted the highest protein contents(29.931 mg/g),with many electrophoretic bands and clear background. When mercaptoethanol method was used as optimal extraction method,electrophoresis effects were the best in the conditions of materials-lipid ratio of 1:10,sample volume of 5 times,separate gel concentration of 15%,which obtained 18 bands totally. CONCLUSIONS:Established protein extraction method and SDS-PAGE technical system are suitable for detecting the purity of A. sinensis seed.

Objective To study the effects of alternating magnetic field with different intensities on the proliferation of human squa-mous tongue cancer cells in vitro. Methods Viable cells with the OD value cell and flow cytometry were revealed through MTT assay to evaluate the proliferation and apeptosis and cell cycle respectively after the cells were exposed to electromagnetic fields of different intensity (5mT,8mT,11mT) once per day lasting 1 hour for 3 days. The sham -exposure controls were correspondingly established. Results We compared the electromagnetic field groups with the normal groups by MTT assay after 24,48,72 hours. By analyzing the data in SPSS sta-tistical software , we found that the OD value of electromagnetic field groups was significantly less than that of the control groups (P <0.01) . The rates of apoptosis cells by flow cytometry revealed that EMF groups had no change as compared with control groups. But the cell cycle displayed significant chang at 0.5rot. Conclusion The cells displayed significant changes with obvious Tca8113 cell prolifera-tion inhibition and hold - up cell cycle after being exposed to alternating magnetic field of different intensity. But human squamous tongue cancer ceils could not be induced to apeptosis.

Purpose:To investigate the relationship between inactivity of mismatch repair system in colorectal cancer (CRC) in Chinese patients and their age at onset of CRC.Methods:Genomic DNA extracted from colorectal cancer tissues and their normal colon tissues were subjected to analysis for microstallite instability (MSI) in six of DNA markers in 100 colorectal cancer patients.Results:Microsatellite instability positive (MSI + ) was detected in 46 out of 100 (46%) of CRC tissues, MSI + H in 18/100 (18%) and MSI + L in 28/100 (28%). The rate of MSI + in the CRC patients who are younger than 45 years old is higher than that in older patients (≥65 years ) ( P

Magnetic induction hyperthermia becomes a very important tumor treatment method at present. In order to ensure a successful operation, doctors should make hyperthermia treatment planning before surgery. Based on Integration Healthcare Enterprise (IHE) framework and Digital Imaging and Communications in Medcine (DICOM) standard, we proposed and carried out a network workflow integrated with modern medical information systems for the dissemination of information in magnetic induction hyperthermia like accurate accessing patient information and radiology image data, storing processed images, sharing and verifying hyperthermia reports. The results proved that our system could not only improve the efficiency of magnetic induction hyperthermia treatment planning, but also save medical resources and reduce labor costs.
Humans ; Hyperthermia, Induced ; Image Processing, Computer-Assisted ; Magnetic Phenomena ; Neoplasms ; therapy ; Radiology Information Systems ; Systems Integration

BACKGROUND:Now most of the researches on transplant hair are based on the patients with enough hair, but there is no apparent corrective effect for large area of alopecia. Implanted artificial hairs can solve this problem. OBJECTIVE:To make and evaluate the γ-aminopropyl triethoxysilane/polypyrrole/polyester (KH-550/PPy/PET) composite monofilament as implanted artificial hairs, and to carry out cytotoxicity tests using NCTC clone 929 cels with composite monofilament.
METHODS:KH-550/PPy/PET composite monofilament was prepared in a series of steps, including pre-spotting, alkali treatment, silane coupling agent treatment and polypyrrole coating. The viability of NCTC clone 929 cels were detected after 1, 2, 3, 5, 7 days of co-culture with composite monofilament by using cellcounting kit-8.
RESULTS AND CONCLUSION:KH-550/PPy/PET composite monofilament had a smooth surface without crack. The PPy films did not come off accidentaly and had good wearability. After alkali treatment, PPy quality on the surface of monofilament was significantly heavier than before. Using silane coupling agent (KH-550) could effectively enhance biocompatibility and binding force of polyester monofilaments. After co-cultured with composite monofilaments, the viability of NCTC clone 929 cels was 100%, 80.37%, 73.26%, 81.96%, 77.50% at days 1, 2, 3, 5, 7 respectively. The level of cytotoxicity was grade 1. The results show that KH-550 can effectively enhance the binding force between PPy and PET monofilament, and the prepared KH-550/PPy/PET composite monofilament has good biocompatibility and no acute toxicity.

Purpose The main purposes of our research were to: 1. set up the method of the RGDHirudin microsphere preparation; 2. set up the method to test the activity and the content of the medicine contained in the microsphere; 3. analyse the key factors on the quality of the microsphere preparation. Methods Co-poly lactic acid glycolic acid (PLGA) microsphere was prepared by a modified solvent evaporation method by a double emulsion with the use of polyvinylalcohol (PVA) as emulsification; PLGA was used as biodegradable material and dichloromethane as organic solvent. The influence of formulation factors including the W1/O on microsphere diameter distribution and yield coefficient;PVA concentration on microsphere appearance, encapsulation and yield coefficient; ultrasound on spherulization average and medicine activity; stirring speed on spherulization average and microsphere appearance; PLGA on microsphere appearance and microsphere dispersity; concentration of NaCl on encapsulation efficiency, yield coefficient and medicine content etc were studied. Results The size of all the fabricated microsphere was measured according to the several factors that affect the particle size. The average diameter was 81.38 μm, which is good for further research. The medicine content and the percent yield of all the microsphere was high, which ranged from 83. 92% - 96. 3% and 79.93% - 95.05% respectively. The encapsulation efficiency was about 23.95% - 65. 13%. We found that the concentration of the NaCl and PVA were the very important factors to the encapsulation efficiency. Physiological activity of RGD-Hirudin containing in the microsphere and the release rate of the microsphere were controlled. Furthermore, the release rate was stable. Conclusions The physiologic activity of RGD-Hirudin released from the microspheres was stable. PLGA-RGD-Hirudin microspheres were controlled released by the in vitro studies. Therefore, the in vivo experiment was well grounded.

BACKGROUND:Magnetic bone cements have been used to treat bone metastasis in Japan, which are made by adding Fe3O4 nanoparticles to bone cements. Magnetic bone cements containing micron carbonyl iron powder have not been reported.
OBJECTIVE:To prepare polymethylmethacrylate-based cements containing carbonyl iron powder, and to test the magnetic characterizations and the heat-generating abilities of al samples according to ISO 5833 standard in vitro. METHODS:The carbonyl iron powder was mixed with polymethylmethacrylate-based bone cement power to prepare magnetic bone cements containing 0%, 20%, 30%, 40%, and 50%carbonyl iron powder, respectively. The setting time, polymerization temperature, compressive strength, magnetic property and in vitro heat-generating ability were tested.
RESULTS AND CONCLUSION:The setting time and polymerization temperature were increased with the increased content of carbonyl iron powder. The highest polymerization temperature of each sample was 65-70 ℃. The increased content of carbonyl iron power could not change the highest polymerization temperature but delay its appearance. The compressive strength of each sample was higher than 60 MPa, and moreover, the compressive strength of the pure polymethylmethacrylate-based bone cement was higher than 60 MPa, which met the ISO 5833 standard. The saturation magnetic intensity was increased with the increasing of carbonyl iron power content. The heat-generating ability of magnetic bone cements had a positive correlation with the magnetic field strength and the content of carbonyl iron powder.

The aim of this study is to investigate the critical factor affecting the properties of PLGA microspheres fabricated by a solid-in-oil-in-water (S/O/W) emulsion technique with BSA as a model protein. Prior to encapsulation, the BSA microparticles were fabricated by a modified freezing-induced phase separation method. The microparticles were subsequently encapsulated into PLGA microspheres by S/O/W emulsion method, then Motic BA200 biological microscope, confocal laser scanning microscope, scanning electron microscope were used to observe the structure of S/O/W emulsion and PLGA microspheres. The protein content extracted or released from BSA microspheres was measured by Bradford protein assay method. It was found that NaCl added in the outer aqueous phase effectively suppressed material exchange between the inner and outer phase of S/O/W emulsion. Then, the structure and permeability of obtained microspheres were influenced. As a result, with the increase of NaCl concentration in the outer aqueous phase, the encapsulation efficiency of microspheres significantly increased from 60% to more than 85%, the burst release of microspheres reduced from 70% to 20%, and the particle size decreased from 103 microm to 62 microm. Furthermore, the rehydration of encapsulated protein was also retarded and then integrity of BSA was successfully protected during encapsulation process. In vitro release test showed that BSA released from PLGA microspheres in a sustained manner for more than 30 days.