1.Indwelling Venous Catheter-related Infection in Artificial Liver Support System:Clinical Analysis of 61 Patients
Jie JIN ; Weijiang YE ; Jinsong HUANG ; Xiaoling GE ; Haiyan YU
Chinese Journal of Nosocomiology 2006;0(09):-
OBJECTIVE To analyze clinical and etiologic characteristics of indwelling venous catheter-related infection(CRI) in artificial liver support system(ALSS)-treated patients and explore the measures of prevention and treatment.METHODS Bacterial culture and drug sensitivity test were performed in cusp of catheters after being pulled out and the peripheral blood in liver failure patients.RESULTS Sixty four strains were isolated including 56 Gram-positive strains,2 Gram-negative strains and 6 fungi ones,respectively.The most frequent organism was Staphylococcus epidermidis which had high sensitivity to vancomycin.CONCLUSIONS The most effective measure is removing catheters in time,and vancomycin is the most optimal agent for CRI.
2.Reform practice of PBL teaching model in organ system integrated course
Jinsong YE ; Yanqing XIE ; Lixia ZHENG ; Fengling CHEN
Chinese Journal of Medical Education Research 2021;20(3):271-274
Problem-based learning (PBL), which originated in foreign countries, advocates the core idea of multidisciplinary integrated learning, which coincides with the trend of curriculum integration in current medical education. First, this article systematically introduces the process of PBL in the organ system integrated course which is conducted in Nanshan College of Guangzhou Medical University. Second, this article discusses the teaching results and reflection so as to provide reference for the continuous localization reformation of PBL and further medical curriculum integration in college and university.
3.Research progress of aptamers application in molecular imaging.
Journal of Biomedical Engineering 2012;29(6):1230-1234
Molecular imaging technology, an advanced research area of imaging, can provide real-time, non-invasive image information of the target site in molecular level. The key of the molecular imaging technology is molecular probe. Aptamers are short oligonucleotides with high affinity and specificity to the target molecules. The targeting ability, stability and safety of aptamers are superior to traditional antibodies so that aptamers show prosperous usages in targeting drug delivery and disease diagnostics. Therefore, aptamers are considered to be an extremely ideal probes, which can guide quantum dots, magnetic nanoparticles and ultrasound contrast agents on the targets and realize optical, magnetic resonance, ultrasonic multimodal and multifunctional imaging. All of the advantages can further promote the application of molecular imaging in disease treatment and diagnosis. In this paper, we review recent developments in the application of aptamers as molecular probes in major branches of molecular imaging.
Animals
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Aptamers, Nucleotide
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Contrast Media
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Humans
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Molecular Imaging
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methods
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trends
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Molecular Probes
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SELEX Aptamer Technique
4.Analysis and thinking of present situation of general practitioner job-transfer training
Ye WU ; Donghua ZHANG ; Hong BO ; Jinsong GUO ; Jingzhu DONG ; Liying LAN
Chinese Journal of Medical Education Research 2013;(3):298-301
Job-transfer training for general practitioner is the important channel of primary medical talents team construction.This article elaborated on existing problems in the general practitioner job-transfer training in our country from several aspects,such as training base,teachers,teaching materials,actual work need and discipline construction,etc.Based on the existing problems and combined with practical work requirements of general practitioner job-transfer training in our country,the article put forward corresponding suggestions and countermeasures including strengthening teachers' construction,textbook construction and speeding up general medical discipline construction,etc.
5.Therapeutic effects of blood purification in 160 cases with liver failure
Weijiang YE ; Jie JIN ; Haiyan YU ; Jie WANG ; Xin LUO ; Jinsong HUANG
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2006;0(06):-
Objective: To evaluate the clinical efficacy of various methods of blood purification in the treatment of patients with liver failure.Methods: Two hundred and seventy-three patients suffering from liver failure were randomly divided into two groups.On the base of medical routine treatment,160 patients were additionally treated with various methods of blood purification treatment group,including plasma exchange,plasma exchange associated with continuously venovenous hemofiltration,plasma exchange associated with hemodiafiltration,plasma exchange associated with plasma absorption,molecular absorbents recycling(system) and so on.While the other 113 patients received medical routine treatment only(control group).The efficacy of treatment and prognosis in the two groups were then observed and compared.Results: In the treatment group,the total bilirubin(TB),albumin(ALB),total bile acid(TBA),alanine aminotransferase(ALT),pre-albumin(PALB),cholinesterase(CHE) and prothrombin activity(PTA) after the treatment were better compared with those before the treatment(all P
6.Practices of a hospital to control of medical expenses
Chinese Journal of Hospital Administration 2018;34(5):400-402
Measures have been taken by the hospital to control drug expense proportion and consumables expense proportion, in addition to such actions as ceiling pricing for individual diseases, clinical pathways and better incentive mechanisms. All these measures help to curb unreasonable growth of medical expenses, and ease patients' burden. The study proves that the goal of expense control set in the healthcare reform can be achieved by setting multiple control indexes, strengthening pharmaceutical affairs management, normalizing procedure of consumables circulation, reforming the compensation incentive mechanism, giving the best play of cost control role of medical insurance, and introducing new technologies or programs.
7.Detection of human herpes virus 8 in Kaposi sarcoma using polymerase chain reaction and single stranded probe in situ hybridization with a tyramide signal amplification system.
Xiaoge ZHOU ; Jinsong ZHANG ; Xiaoping ZHANG ; Ye ZHANG ; Kristian SANDVEJ ; Stephen HAMILTON-DUTOIT
Chinese Journal of Pathology 2002;31(4):309-313
OBJECTIVETo study the relationship between Kaposi's sarcoma (KS) and human herpes virus 8 (HHV8; Kaposi's sarcoma-associated herpes virus), and to develop an in situ hybridization (ISH) technique effective for clinical pathological diagnosis.
METHODSPolymerase chain reaction (PCR) technique was used to synthesize a digoxigenin-labeled single stranded DNA probe specific for HHV8 open reading frame 72 cyclin D homolog gene encoded mRNA as the probe accompanying with a tyramide signal amplification system (TSA) for ISH assay. Totally 20 formalin-fixed and paraffin-embedded samples from 14 cases of KS from Danish patients were collected for HHV8 detection. In order to compare the result obtained, all of these cases were checked simultaneously using PCR technique.
RESULTSHHV8 was detected in 10 of 14 (71%) KS cases, of which 8 cases were positive for HHV8 by both ISH and PCR. In ISH, HHV8 hybridization signal was seen as a dot or patch located in the nuclei of both the neoplastic spindle cells and the endothelial cells. HHV8 was found in lesions in all the stages of KS including early patch, plaque and late nodular or tumor lesions, as well as in the primary and metastatic lesions. All the control cases showed a relevant positive or negative results.
CONCLUSIONSThe results further confirmed that there is a strong association between Kaposi's sarcoma and HHV8. The ISH technique with single stranded probe and TSA would be helpful in clinical pathological diagnosis for HHV8 infected diseases, such as KS, primary effusion lymphoma and multicentric Castleman's disease.
Adolescent ; Adult ; Female ; Herpesvirus 8, Human ; isolation & purification ; Humans ; In Situ Hybridization ; methods ; Male ; Middle Aged ; Polymerase Chain Reaction ; methods ; Sarcoma, Kaposi ; pathology ; virology ; Tyramine
8.The Clinic Study on Synchronous Detection of HCV RNA in the Plasma and PBMC of Hepatitis C Patients
Jin YE ; Wei LIU ; Jinsong GUO ; Linglan ZENG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2001;21(2):110-111
In order to increase the positive detection rate of HCV RNA in the patients with chronic hepatitis C (CHC), RT-PCR was used to synchronously detect HCV RNA in the plasma and peripheral blood mononuclear cells (PBMC) of 583 CHC patients with a continuously elevated level of ALT for more than one year. The results showed that the positive detection rate of HCV RNA in the plasma of the CHC patients was 19.2 %, while 24.5 % in PBMC. It was demonstrated that the positive detection rate for HCV RNA in PBMC was obviously higher than that detected in plasma. To synchronously detect HCV RNA in PBMC by using RT-PCR can increase the positive detection rate of HCV RNA in the CHC patients.
9.The Clinic Study on Synchronous Detection of HCV RNA in the Plasma and PBMC of Hepatitis C Patients
Jin YE ; Wei LIU ; Jinsong GUO ; Linglan ZENG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2001;21(2):110-111
In order to increase the positive detection rate of HCV RNA in the patients with chronic hepatitis C (CHC), RT-PCR was used to synchronously detect HCV RNA in the plasma and peripheral blood mononuclear cells (PBMC) of 583 CHC patients with a continuously elevated level of ALT for more than one year. The results showed that the positive detection rate of HCV RNA in the plasma of the CHC patients was 19.2 %, while 24.5 % in PBMC. It was demonstrated that the positive detection rate for HCV RNA in PBMC was obviously higher than that detected in plasma. To synchronously detect HCV RNA in PBMC by using RT-PCR can increase the positive detection rate of HCV RNA in the CHC patients.
10.Prognostic significance of neutrophil to lymphocyte ratio in patients with hepatocellular carcinoma
Jinsong YE ; Bangde XIANG ; Nengzhi CHEN ; Kelan ZHANG ; Fenghua ZENG ; Tao LUO ; Chong WU ; Zhigang CHEN ; Xiaolong WU ; Yingjun HE ; Wei TIAN ; Haojie YANG
Chinese Journal of Hepatobiliary Surgery 2017;23(3):158-161
Objective To compare the clinical significance of peripheral blood neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) in predicting prognosis of patients with hepatocellular carcinoma (HCC).Methods The clinical data of 661 patients with HCC were retrospectively analyzed.Routine peripheral blood test results were used to calculate the NLR and PLR,and the receiver operating characteristic (ROC) curves were drawn.Using the thresholds of NLR and PLR,the patients were divided into the low NLR group and the high NLR group,and the low PLR group and the high PLR group.Overall survival (OS) and disease free survival (DFS) were evaluated by the Kaplan-Meier method.Independent prognostic predictors were determined by the Cox proportional hazard model.Results The NLR and PLR thresholds were 2.790 and 99,respectively.Analysis of the ROC curves showed higher NLR and PLR were significantly associated with poorer OS and DFS (all P < 0.05).Multivariate analysis showed that NLR was an independent risk factor of OS and DFS (both P < 0.05).The results remained unchanged when the NLR was further analyzed by applying different cut-off values of 2.810 and 3.In subgroup analysis,NLR remained an independent factor of Barcelona Clinical Liver Cancer staging system (BCLC) 0/A/B (P < 0.05 for all measurements).Conclusion An elevated preoperative NLR could be a better prognostic predictor for HCC patients in comparison with PLR,especially for BCLC 0/A/B patients.