Objective To establish an HPLC method for determining gallic acid in Chebulae fructus immaturus of different area.Methods The ZORBAX SB-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile: 0.1％ H3PO4(29: 71),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 273 nm.Results Gallic acid was successfully separated within 20 min,the linear response range was 0.5611～5.611 μg.The average recovery was 99.9％,and RSD was 2.35％.Conclusion The method is simple,accurate and repeatable; it can provide evidence for further development and utilization of this crude drug.

Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Polygonum aviculare L.Methods The Agilent LC-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile:0.1％ H3PO4(18 ∶ 82),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 256 nm.Results The calibration curve was linear within a range of 1.22～24.30 μg/ml and 0.77～15.40 μg/ml,the average recovery of this method was 98.9％,99.6％ and the RSD was 1.11％,1.09％,for the myricetrin and quercitroside respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of myricetrin and quercitroside in Polygonum aviculare L..

Objective:To summarize the experience of surgical treatment of valvular heart disease with enlarged or small left ventricles. Methods:Valvular operations were performed in 473 patients,including 38 with enlarged left ventricles(group Ⅰ)and 40 with small left ventricles(group Ⅱ),from April 1998 to June 2005.Postoperative complications between two groups were com- pared and analyzed statistically. Results:The postoperative eomplications were ventricular arrhythmia,low output syndrome,lung infection,renal failure and brain embolism.Low output syndrome tended to occur in group Ⅱ(20%),whereas there was a higher incidence of ventricular ar- rhythmia in group Ⅰ(65.79%).The difference between groups was significant(P＜0.05). Conclusion:To improve the successful operative rate in patients with enlarged or small left ventricles,we should take effec- tive measures to manage the post-procedural complications,such as ventricular arrhythmia(mainly in group Ⅰ)and low output syndrome(mainly in group Ⅱ).

Objective:To analyze the effect of glucocorticoid on the biological function of lens epithelial cells (LECs) by bioinformatics and predict related microRNA (miRNA).Methods:GSE3040 database was downloaded and the human LECs line (HLE-B3) cells in the experimental group were treated with 1 μmol/L dexamethasone, and HLE-B3 cells in the control group were treated with 1 μmol/L dimethyl sulfoxide(DMSO).GEO2R was used to analyze the differentially expressed genes between the two groups.Metascape website was employed to analyze the functional enrichment of differentially expressed genes, and EdU cell proliferation assay was performed to detect the difference in cell proliferation between the two groups.STRING website and cytoscape software were used to construct protein-protein interaction network.Hub genes were calculated by cytohubba app, and quantitative real-time PCR was performed to detect the expression levels of hub genes between the two groups.MirCode website was used to predict the related miRNAs.Results:A total of 341 differentially expressed genes were detected between the experimental group and the control group, among which there were 300 up-regulated genes and 41 down-regulated genes. SLC12A1, MED13L, ALDH5A1, SLC15A3 and WWC1 were the top five down-regulated genes and SCNN1A, ANKRD36, FKBP5, PYY and ADH1B were the top five up-regulated genes.The top 20 terms of functional enrichment were listed, and the negative regulation of HLE-B3 cells proliferation showed the most enrichment.Cell proliferation rate in the experimental group was (8.09±0.20)%, which was significantly lower than (39.63±0.80)% in the control group ( t=38.43, P<0.01).The top ten hub genes were SST, CXCL8, GRM1, GNRH1, CXCL5, PPBP, CX3CR1, PYY, EDNRA and GRK5, and quantitative real time PCR confirmed that the expression levels of SST, CXCL8, GRM1, PYY, EDNRA and GRK5 mRNA were statistically different (all at P<0.05).The top six miRNAs which might be associated with hub genes were miR-15abc, miR-214, miR-23abc, miR-129-5p, miR-132 and miR-24. Conclusions:The 1 μmol/L glucocorticoid can negatively regulate the proliferation of HLE-B3 cells. SST, CXCL8, GRM1, PYY, EDNRA and GRK5 may be hub genes and miR-15abc, miR-214, miR-23abc, miR-129-5p, miR-132, miR-24 are most likely to relate to them.

Objective To explore the influence of diabetes on the protective effect of ischemic preconditioning(IPC) on ischemic-reperfused(IR) myocardium of rat.Methods Sixty male SD rats(30 with diabetes and 30 without diabetes) were divided into six groups(10 each): non-diabetic rats control group(group A),non-diabetic rats IR group(group B),non-diabetic rats IPC group(group C),diabetic rats control group(group D),diabetic rats IR group(group E) and diabetic rats IPC group(group F).The isolated heart perfusion Langendorff models were established after rats were sacrificed.Isolated hearts of the two control groups were undergone a 90min perfusion without any other intervention;those of the two IR groups were undergone a 30min equilibration period,a 30min ischemia and a 30min reperfusion;those of the two IPC groups were undergone a 10min equilibration,then elicited by two cycles of 5min ischemia interspersed with 5min reperfusion prior to 30min ischemia and a 30min reperfusion.The recovery rates of the left ventricular function,such as cardiac output(CO) and left ventricular developed pressure(LVDP),and the maximum upstroke and decay velocities of left ventricular pressure(?dp/dtmax) were recorded.The activity of creatine kinase(CK) in coronary outflow,contents of malonyldialdehyde(MDA) and superoxide dismutase(SOD) in myocardium were detected,and myocardial water ratio were assessed.Results In non-diabetic rats IPC group,the activity of CK,content of MDA and water ratio of myocardium declined significantly,while the content of SOD increased and the recovery rates of CO,LVDP and ?dp/dtmax rose significantly compared with that in non-diabetic rats IR group(P0.05).Conclusion Diabetes may inhibit the protective effect of ischemic preconditioning on ischemic reperfused rat heart.

Objective To explore the effects of α-keto acid combined with low protein diet on oxidative stress and electrolyte levels in patients with diabetic nephropathy. Methods Ninety-eight cases with type 2 diabetic nephropathy were randomLy divided into the treatment group(low-protein diet with α-keto acid 1 tablet,n=58) and the control group(normal protein intake,n=40). The 24 h urinary albumin excretion rate,glomerular filtra-tion rate ,serum oxidative stress (SOD ,MDA ,AOPPs) and electrolyte levels were measured before and after treatment. Results After 24 weeks of treatment,the 24 h urinary albumin excretion rate of DN patients in the treatment group was significantly lower than that before treatment and the control group (P < 0.05). However, there was no significant change in the glomerular filtration rate of the patients. Compared with the control group , the serum SOD activity of DN patients was significantly increased and the levels of MDA and AOPPs were decreased(P < 0.05)after 24 weeks of treatment. Simultaneously,the levels of serum potassium and phosphorus in the treatment group was decreased ,while the levels of calcium and magnesium were improved (P < 0.05). Conclusion α-keto acid combined with low protein diet can significantly reduce the urinary albumin excretion rate,improve the body′s antioxidant activity,and maintain serum electrolytes balance.

BACKGROUND: The prompt and efficient peripheral blood stem cell (PBSC) mobilization is necessary for repairing and regenerating injured tissues. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) or its combination with a large dose of chemotherapy is usually utilized for PBSC mobilization, but the regimen requires a long time and a complex procedure. OBJECTIVE: To modify the common PBSC mobilization, and investigate mobilization effect of PBSC in KM mice with short-duration and high-dose rhG-CSF. DESIGN, TIME AND SETTING: A randomized controlled animal trial was carried out in the laboratory of Hematology, the Second Affiliated Hospital to Dalian Medical University from September 2006 to March 2007. MATERIALS: Twenty-four KM male mice of 8 months old were divided into 3 groups according to the administration: conventional regimen group, short-duration and high-dose group, and saline control group. METHODS: Mice in the conventional regimen group were injected subcutaneously with rhG-CSF (0.2 ?g one time, twice a day for 6 days). Mice in the short-duration and high-dose group were injected with equal volume of normal saline at first 4 days, and then with rhG-CSF (2.5 ?g one time, twice a day for 2 days). Mice in the saline control group were injected subcutaneously with equal volume of normal saline for 6 days. MAIN OUTCOME MEASURES: Peripheral blood white blood cell (WBC) and colony-forming unit-granulocyte macrophage in KM mice were counted. RESULTS: WBC in the peripheral blood of KM mice increased rapidly in conventional regimen group and short-duration and high-dose group. No obvious change of WBC was observed in the control group. The number of colony-forming unit-granulocyte macrophage in conventional regimen group and short-duration and high-dose group was significantly elevated over 50 folds than control group (P 0.05). CONCLUSION: There is a good efficacy and feasibility in PBSC mobilization of KM mice with short-duration and large-dose rhG-CSF, and it is a successful amelioration of PBSC mobilization of mice peripheral blood.

OBJECTIVE:To screen the formulations of bioadhesive danazol suppository,and to study its drug release in vitro.METHODS:Hydroxypropyl methylcellulse(HPMC),polyethylene glycol(PEG)6000and PEG600were employed in the bioadhesive sustained-release formulation.Suppositories were prepared and the correlation between drug release rate from suppositories and the ratio of HPMC to PEGs used in the formulae was studied.RESULTS:HPMC retarded drug release,and drug gradually released in the12-hours period when the ratio of HPMC to PEGs was1∶6.5;the law of drug release was conformed to weibull models and mono-exponential models.CONCLUSION:HPMC,PEG6000and PEG600were suitable to the formulation of bioadhesive danazol suppositories,and HPMC could slow down the drug release from the this preparation;the best ratio of HPMC to PEGs was1∶6.5.

To evaluate the effect of delayed treatment with monoclonal antibody to tumor necrosis factor-alpha (TNF-? MAb) on systemic hemodynamics and multiple organ dysfunction following prolonged hemorrhagic shock and resuscitation. Method: Adult male Sprague-Dawley rats were subjected to prolonged hemorrhagic shock (MAP of 4.00-4.66 kPa for 180 rain)followed by resuscitation over 50 min. The animals were treated intravenously with either TNF-? MAb (20.0 mg/kg) or the control protein(albumin,20.0 mg/kg)15 rain after the end of resuscitation(65 min after shock). Result: Compared to the albumin controls, delayed treatment with TNF-? MAh significantly reduced the total peripheral resistance index (P

Patients with ataxia-telangiectasia ( A-T) syndrome were charachaterized by profound hypersensitivity to ionizing radiation in clinic. Many studies have shown that this hypersensitivity possibly attributed to ATM gene whose critical compartment was ATM kinase. So inhibitors of the ATM kinase such as caffeine, pentoxifylline, methyl xanthines and 7-hydroxystaurosporine (UCN-01) were developed and have achieved a few encouraging results in basic and clinical stuides.