ObjectiveTo investigate the effect of Jiawei Glutinous decoction on gouty nephropathy. Methods54 patients with gouty nephropathy were randomized into two groups, 31 patients in the treated group were treated with Jiawei Glutinous decoction, and 23 patients in the control group were treated with allopurinol. Blood uricacid (BUA), renal function, β2-microglobulin (β2-MG) and 24 h urine protein of patiens in two groups were measured before and after treatment.ResultsImprovements of symptoms and renal function as well as decreasing of BUA, β2-MG and 24h PRO in the treated group was superior to those in the control(P<0.05).ConclusionJiawei Glutinous decoction present good effect in treating gouty nephropathy.

Objective To investigate the relationship of character of atherosclerotic plaque in carotid artery and serum CRP level. Methods The atherosclerotic plaques in carotid arteries were confirmed by multislice CT angiography(MSCTA),in which serum CRP level were detected in 28 patients with cerebral thrombosis,24 patients with lacunar infarction and 19 patients with TIA in internal carotid artery system in order to analysis the relationship between character of plaque and serum CRP level. Results (1)Mixed plaque and soft plaque were mainly found in cerebral thrombosis and TIA groups, while rigidity plaque was mostly found in lacunar infarction group, there were significance difference in plaques distribution among three groups(all P0.05).(3) CRP level in different groups was (4.546?2.720)mg/L in soft plaque group, (3.951?1.863)mg/L in mixed plaque group respectively,those were both higher than that of the rigidity plaque group[(2.762?1.323)mg/L],and there was significance difference between them(all P

Objective To investigate the indoor air formaldehyde pollution in the human anatomy laboratory and to study the control measures and their effects on prevention and control of formaldehyde pollution. Methods The levels of formaldehyde in the air of the human anatomy laboratory with exhaust ventilatary conditional system and without such a system were determined respectively according to the method recommended in the Sanitary Standard of Public Place. Results Without the exhaust ventilatory conditional system, the level of formaldehyde of indoor air reached to 8.349 mg/m3, while the level of formaldehyde was decreased significantly to 0.501 mg/m3 with the system. Conclusion Without the exhaust ventilatory conditional system, the formaldehyde pollution in the air of the human anatomy laboratory was very serious, which might have adverse effects on teachers' and students' health, while with the system, the formaldehyde pollution could be controlled effectively.

Objective To observe heterotopic osteogenesis of autogenous marrow stromal cells on ?-tricalcium phosphate (?-TCP) scaffold, and the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) and transforming growth factor-beta (TGF-?) on osteogenesis. Methods The Newzealand rabbits autogenous marrow stromal cells were cultured by mineralized condition medium. The cell suspension was collected. All the cells with concentration of 5 ?106 ? mL-1 were divided into four groups. Group A: rhBMP-2 (15 ?g) /TGF-? (30 ng) were added; group B: TGF-? 30 ng was administered; group C: without growth factor as control; group D: continual cultured and no loading on ?-TCP. MSCs in group A, B, C were planted on ?-TCP. Alkaline phosphatase (ALP) activity was examined by histochemistry. Then MSCs loading on ?-TCP were embedded in autogenous subcutaneous. Four weeks later, the osteogenesis was observed by HE staining, type I collagen and bone morphogenetic protein synthesis were examined by immunohistochemistry stains. Results The ALP activities in groups A and B were significantly higher than those in group C and D (P

Objective To observe arthroscopic manifestations of necrosis of the femoral head and to evaluate the results. Methods Arthroscopic operations were performed in 38 hips in 27 patients (19 male and 8 female) with necrosis of the femoral head. There were 8 hips of Ficat stage Ⅰ, 21 hips of stage Ⅱ, 7 hips of stage Ⅲ, and 2 hips of stage Ⅳ, respectively. The arthroscopic operation included an assessment of the appearance and texture of the femoral head, the acetabulum, and the synovium, debridement of the joint, and femoral head decompression by drilling small holes. Results Arthroscopically, the Ficat stage I hips were considered to have a intact articular surface, with normal appearance and instrument compression, displaying a hyperaemia and swelling of the synovial tissue. The stage Ⅱ hips presented a major portion of the normal articular surface, chondromalacia of the weight bearing portion of the femoral head, fissures on the articular surface, and sinking with instrument compression. A cleavage plane was formed between the fractured and collapsed necrotic subchondral bone, but the cartilage still communicated with the calcificated layer. The surface of necrotic bone was sclerosesed and rough like sandstones. Hyperplasia of the synovial tissue was seen. The stage Ⅲ hips manifested a massive disassociation of the articular surface from the underlying subchondral bone and the delamination of the femoral head. The subchondral bone was collapsed and disintegrated, with ineffective repaired granulation tissue in it. The stage Ⅳ hips displayed narrowing of the joint space, extensive chondral lesions of the femoral head, collapse of necrotic subchondral bone exposed, and degenerative changes on the acetabulum side, all of which were consistent with the end-stage of osteoarthritis. Conclusions Articular cartilage impairment plays an important role in the process of the necrosis of the femoral head. The value of hip arthroscopic examinations lies in accurate evaluation of the extent of the chondral impairment and important reference for the choice of procedures.

Aim To study the effect of proteasome inhibitor MG132 on the expression of caspase-3 and apoptosis in cultured human umbilical vein endothelial cells.Methods Human umbilical vein endothelial cells was treated with MG132 (2,5 ?mol?L~(-1)) for 24 h. The apoptotic cells were determined by DNA fragment analysis and flow cytometric analysis. The level of caspase-3 mRNA was quantified by reverse transcription-polymerase chain reaction (RT-PCR). The protein contents of caspase-3 were analyzed by immunocytochemistry.Results The results showed that the increase of the degree of human umbilical vein endothelial cells apoptosis was concentration dependent. MG132 could up-regulate the gene/protein expression of caspase-3.Conclusions The results implicated that proteasome inhibitor MG132 induced human umbilical vein endothelial cells apoptosis by accumulation of caspase-3.

Objective To observe the effects of Okinawa Habu apoxin protein-1 (OHAP-1) on the proliferation inhibition of rat C6 glioma cells and its mechanisms. Methods MTT colorimetric analysis was used to measure the inhibitory effect of OHAP-1 with different doses(2.5,5.0,and 10.0 mg?L-1) on C6 glioma cells .RT-PCR was used to evaluate the mRNA expressions of bcl-2 and bax genes.The activity of superoxide dismutase (SOD) and the level of maleicdialdehyde (MDA) in the C6 glioma cells were also examined. Results The proliferation of C6 glioma cells was significantly inhibited by different doses of OHAP-1(2.5,5.0,and 10.0 mg?L-1).The inhibitory rate were 49.77%,67.65%,and 76.42%,respectively.The inhibitory rate in 2.5,5.0, and 10.0 mg?L-1 groups were higher than that in control group(P

Objective To investigate the expression of Notch1 and its ligand DLL4 in human gastric carcinoma tissues and its correlation with tumor angiogenic metastasis.Methods Immunohistochemical SP method was used to detect the expression of Notch1,DLL4 and VEGF in 45 gastric carcinoma tissues and paired adjacent normal gastric mucosa,and the relationship between them and clinico-pathological parameters were analyzed.Results The positive expression rate of Notch1,DLL4 and VEGF in gastric carcinoma were higher than that in normal gastric mucosa(P

Objective To compare the effects of three kinds of fixative solutions on paraffin section of mouse lens tissue and optimize the fixing?method of paraffin section in mouse lens tissue. Methods Three kinds of conventional fixatives were selected for the test ,including the conven?tional Davison’s solution,modified Davison’s solution and 10%neutral buffered formalin. Mice eyeball tissues were fixed with three different fixa?tives,embedded,sliced and then stained with HE method. The paraffin slices were observed under the light microscope. Results The structures of lens and retina fixed in conventional Davison ’s fixative solutions were clear and intact ,and the cells were arranged regularly and compactly. There was no eyeball distortion,contraction and retinal detachment in the eyeballs fixed in modified and conventional Davison’s fixative solution. However,the ones fixed in 10%neutral buffered formalin showed eyeball distortion and contraction,space and spherules. Conclusion The mice lens slides made from tissues fixed by conventional Davison ’s fixative solution are better than fixed by modified Davison ’s fixative solution and the 10%neutral buffered formalin fixed ones.

Objective To investigate the radiation doses for the patients undergoing interventional radiology and to analyze the dose - influencing factors.MethodsThe clinical data of 461 patients undergoing interventional radiology,including cerebral angiography ( CEA ),cerebral aneurysm embolism ( CAE ),superselective hepatic arterial chemoembolization ( SHAG ),coronary angiography ( COA ),percutaneous intracoronary stent implantation ( PIS1 ),cardiac radiofrequency catheter ablation ( RFCA ),and permanent cardiac pacemaker implantation(PCPI) were collected to observe the cumulative air kerma (CAK),dose area product (DAP),and fluoroscopy time,and effective dose was estimated using the conversion factors.Results The effective doses for CEA,CAE,SHAG,COA,PISI,RFCA,and PCPI were (0.33 ±0.20),(0.49 ±0.35),(6.92 ±4.19),(0.76 ±0.91),(2.35 ± 1.47),(0.50 ±0.74),and (0.67 ±0.70) Sv,respectively.In 126 of the 416 patients (26%),the effective doses were greater than 1 Sv,and the effective doses of 10 person-times were greater than 10 Sv,all of which were observed in the patients undergoing SHAG.The CAK values for CEA,CAE,SHAG,COA,PISI,RFCA,and PCPIwere (0.55 ±0.43),(1.34 ± 1.11),(0.95 ±0.57),(0.32 ±0.31),(0.91 ±0.33),(0.16 ±0.22),and (0.15 ±0.14) Gy,respectively.The CAK values were greater than 1 Gy in 59 of the 461 patients ( 12.8% ),greater than 2 Gy in 11 cases (2.4%) ,and greater than 3 Gy in 1 CEA cases and 1 CEA case,respectively.Conclusions There is a wide variation range in radiation dose for different procedures.As most interventional radiology procedure can result in clinically significant radiation dose to the patient,stricter dose control should be carried out.