Objective To analyze the level of blood lipid in healthy Tibetans and Han natives in Lasa through the detection of serum lipids. Methods The serum TC, LDL-C, HDL-C and TG were detected by Hitachi 7060 automatic biochemical analyzer in 13 037 healthy adults (8 163 Tibetan individuals and 4 874 Han individuals). After that, all data were statistically analyzed by age (Tibetan:298 cases of ＜25 year-old group, 1 136 cases of 25-year-old group, 2 039 cases of 35-year-old group, 2 119 cases of 45-year-old group, 1 580 cases of 55-year-old group, 905 cases of 65-year-old group, 86 cases of ≥75 year-old group; Han population:307 cases of ＜25 year-old group, 1 254 cases of 25-year-old group, 1 874 cases of 35-year-old group, 1 022 cases of 45-year-old group, 272 cases of 55-year-old group, 129 cases of 65-year-old group, 16 cases of≥75 year-old group), sex (Tibetan: men 4 505 cases, women 3 658 cases; Han population:men 2 976 cases,women 1 898 cases). Results The average TC, HDL-C and LDL-C levels of Tibetans were (5.07 ± 1.10), ( 1.54 ±0. 49) and (3.33 ± 1.31 ) mmol/L respectively while the average levels of Han natives in Lasa were (4. 60 ± 0. 98 ), ( 1.45 ± 0. 44 ) and ( 2. 85 ± 1.0g ) mmol/L, which showed the levels of the Tibetans were higher than the Han natives in each age group(t =24. 78, 10. 53,21.46, P＜0.05). The TG levels was 1.38 ( 0. 20-2. 99 ) mmol/L in Tibetans and 1.54 (0.20-2.99)mmol/L in Han natives ( F = 224. 88, P ＜ 0. 05 ). The prevalence of total dyslipidemia was 75. 32% in Tibetans and 69. 02% in Han natives of Lasa. The prevalence of total dyslipidemia was 78. 56% in male and 65.42% in female. There were significant differences in both ethic and sex groups(x2 = 9. 678, 44. 138,P ＜0. 05). Conclusion The TC, LDL-C levels and prevalence of dylipidemia in Tibetans are higher than the Han residents in Lasa.

ObjectiveTo assess gene chip application value in detecting pathogenic bacteria in intracranial infection cases.MethodsPrimers and probes aiming at the specific DNA sequences of 4 kinds of common pathogenic bacteria and 6 kinds of common drug resistance genes (DRGs) were designed and used to identify the bacteria and DRGs among 30 cerebrospinal fluid (CSF) specimens (12 positive,18negative in CSF culture) from patients with intracranial infection using multiplex polymerase chain reaction (mPCR) and gene chip.The results of gene detection were compared with those of CSF culture and drug sensitivity testing.ResultsBacteria were identified and DRGs were detected in 15 specimens; DRGs and 16S gene were detected in 8 specimens; neither bacterium nor DRG was detected in 7 specimens.ConclusionGene chip technique is characterized by its relative sensitivity and rapidity of detecting the pathogenic bacteria in CSF of intraeranial infection cases.

Objective To investigate the correlation between blood pressure rhythm and left ventricular structure and function in elderly hypertensive patients. Methods A total of 147 elderly patients with high blood pressure in the First Hospital of Longyan Affiliated to Fujian Medical University were selected. All the patients received 24h ambulatory blood pressure examination. According to the rhythm of blood pressure, the patients were divided into the dipper blood pressure group,the-non dipper type blood pressure group and the anti-dipper type blood pressure group. All patients were examined by echocardiography. Results According to the results of 24h dynamic blood pressure,the type of dipper blood pressure accounted for 11.56% (17 cases) in 147 elderly patients, non-dipper type blood pressure type accounted for 51.02% (75 cases),and the anti-dipper type of blood pressure type accounted for 37.41% (55 cases).The ventricular septal thickness(IVST),left ventricular diastolic inner diameter (LVEDD),left atrium inner diameter(LAD),left ventricle posterior wall thickness( LVPWT) and left ventricle mass index(LVMI) of the non-dipper blood pressure group were (10.56 ± 1.51)mm,(50.17 ± 4.31) mm,(34.65 ± 5.78)mm,(9.26 ± 0.98)mm,(102.31 ± 23.23)g/m2 ,respectively.The IVST,LVEDD,LAD,LVPWT and LVMI of the anti-dipper blood pressure group were (10.51 ± 1.86)mm,(50.20 ± 3.66)mm,(36.96 ± 4.22)mm,(9.42 ± 0.99)mm,(110.47 ± 31.96) g/m2 ,respectively.The IVST,LVEDD,LAD,LVPWT and LVMI of the dipper blood pressure group were (9.53 ± 1.53) mm,(47.59 ± 2.27) mm,(30.47 ± 4.17) mm,(8.88 ± 1.12) mm,(84.98 ± 15.48) g/m2 , respectively. The differences of IVST, LVEDD, LAD, LVPWT and LVMI in the three groups were statistically significant(F=1.172,3.428,1.006,0.135,all P<0.05).The maximum blood flow velocity in early diastolic period of mitral valve blood flow spectrum(E peak)/maximum blood flow velocity in late diastolic period(A peak)(E/A)of the non-dipper blood pressure group and anti-dipper blood pressure group were (0.89 ± 0.30), (0.80 ± 0.28),respectively,which was significantly lower than that of dipper blood pressure group [(1.35 ± 0.63)] (t= -2.890,-3.440,all P<0.05).The left ventricular ejection score(LVEF) of the anti-dipper blood pressure group was (65.31 ± 6.74)% ,which was significantly lower than that of the dipper blood pressure group[(70.12 ± 10.76)% ],the difference was statistically significant(t= -2.209,P<0.05).The 24 h mean systolic pressure,24 h mean diastolic pressure and daytime mean diastolic pressure in the three groups of dynamic blood pressure parameters had no statistically significant differences (all P>0.05).The average daytime systolic pressure in the dipper blood pressure group was (143.06 ± 13.70) mmHg,which was higher than that in the non-dipper blood pressure group [(133.25 ± 13.28)mmHg] and anti-dipper blood pressure group[(131.16 ± 12.26)mmHg],the differences were statistically significant(t= -2.734,-3.401,all P <0.05).The mean evening systolic pressure and the average nocturnal diastolic pressure of anti -dipper blood pressure group were ( 139.04 ± 15.01 ) mmHg and ( 80.18 ± 10.29) mmHg, respectively, which were higher than those of the dipper and non - dipper blood pressure group [(123.24 ± 14.49)mmHg and (72.24 ± 7.97) mmHg,(127.40 ± 13.30) mmHg,(73.45 ± 11.43) mmHg],the differences were statistically significant ( t =3. 822, 4. 666, 2. 919, 3. 456, all P <0. 05 ). LVMI was positively correlated with age,body mass index(BMI),low density lipoprotein( LDL-C),daytime average systolic pressure, night average systolic pressure,night average diastolic pressure,and 24h average systolic pressure(r=0.256,0.241, 0.687,0.251,0.380,0.203,0.243,all P <0.05). Conclusion Anti -dipper blood pressure and non -dipper blood pressure have more significant damage to cardiac function and structure than dipper blood pressure in elderly patients with hypertension,and the elevation of nocturnal blood pressure is closely related to left heart structure and function damage.There is a high correlation between abnormal circadian rhythm of blood pressure and left ventricular hypertrophy in elderly hypertensive patients.

Objective:To investigate the prediction value of observed to expected lung area to head circumference ratio (o/e LHR), measured at different gestational age with various methods, on indication for extracorporeal membrane oxygenation (ECMO) in fetuses with isolated left-sided congenital diaphragmatic hernia (CDH).Methods:Clinical data of 40 neonates who were diagnosed with left-sided CDH and treated in Guangzhou Women and Children's Medical Center were retrospectively collected from January 2017 to May 2021. The o/e LHRs were prenatally calculated using maximum diameter and tracing method at 22-24 and 31-33 weeks of gestation. According to whether the neonates had indications for ECMO after birth or not, they were divided into ECMO ( n=12) or non-ECMO group ( n=28). Differences in the o/e LHR and general situations between the two groups were analyzed using C hi-square test, independent sample t-test, and non-parametric Mann-Whitney U test. Binary logistic regression was used to analyze the influencing factors for ECMO requirement and receiver operating characteristic (ROC) curve was used to evaluate the value of o/e LHR in predicting the indication for ECMO. Results:Both maximum diameter and tracing method suggested that the o/e LHR at 31-33 gestational weeks was lower than that at 22-24 gestational weeks [maximun diameter method: 40.4 (32.9-51.5) vs 45.1 (36.3-53.4), Z=－2.48, P=0.013; tracing method: 38.6 (33.2-47.6) vs 44.1 (35.9-51.7), Z=－3.29, P=0.001]. There was no statistical difference in o/e LHR detected at the same gestational weeks between the two methods (both P>0.05). Binary logistic regression showed that o/e LHR measured at 31-33 gestational weeks using maximum diameter method was an independent protective factor for ECMO requirement ( OR=0.873, 95% CI: 0.790-0.965, P=0.008). ROC curve analysis showed that the area under the curve for evaluating the predictive value of o/e LHR for ECMO requirement was 0.830 with the sensitivity of 83.3% and the specificity of 71.4% when the cut-off value of o/e LHR at 31-33 gestational weeks was 38.195 measured by maximum diameter method. Conclusions:The o/e LHR measured at 31-33 weeks is lower than that at 22-24 weeks of gestation by both methods. The o/e LHR measured by maximum diameter method at 31-33 weeks of gestation may be useful for predicting the ECMO indication after birth but requiring comprehensive evaluation of clinical conditions due to its insufficient predicting power.

Objective:To assess the association between visual-related quality of life (QOL) and the severity of quick contrast sensitivity function (qCSF) defects in patients with early cataract.Methods:A cross section design was performed.Ninety-two eyes of 46 patients with early cataract were enrolled in Zhongshan Ophthalmic Center from March to June in 2017.All subjects completed the Chinese version of the National Eye Institute Visual Function Questionnaire (NEI VFQ-25). The qCSF measurements were performed monocularly and binocularly while the patients wearing full spectacle correction spectacles.Rasch analysis was performed to obtain final NEI VFQ-25 scores.Best corrected visual acuity (BCVA)(LogMAR) was measured and the eye with the better or the worse visual acuity was defined accordingly.The correlation between the Rasch calibrated QOL composite scores and the qCSF measurements of the better eye, the worse eye and binoculus, including the area under Log contrast sensitivity function (AULCSF), qCSF under cut-off frequency and 1.0, 1.5, 3.0, 6.0, 12.0, 18.0 c/d were analyzed.This study protocol was approved by the Ethic Committee of Zhongshan Ophthalmic Center.This study complied with Declaration of Helsinki.Results:The Rasch calibrated QOL composite score was 64.13±4.76.Significant negative correlations were found between composite scores of QOL and the BCVA of both the better or worse eye(the better eye: r=-0.386, P=0.008; the worse eye: r=-0.413, P=0.004). Significant positive correlations were found between the composite scores of QOL and the AULCSF of binoculus, the better and worse eye (binoculus: r=0.531, P<0.001; the better eye: r=0.524, P<0.001; the worse eye: r=0.711, P<0.001); Significant positive correlations were found between the composite scores of QOL and the qCSF under cut-off of binoculus, the better and worse eye (binoculus: r=0.504; the better eye: r=0.419; the worse eye: r=0.694, P<0.01). The composite scores of QOL was positively correlated with the qCSF under 1.5, 3.0, 6.0, 12.0 c/d of binoculus, the better and worse eye (binoculus: r=0.444, 0.544, 0.545, 0.493; all at P<0.05; the better eye: r=0.374, 0.506, 0.530, 0.431; all at P<0.05; the worse eye: r=0.513, 0.679, 0.710, 0.558; all at P<0.05). The composite scores of QOL was positively correlated with the qCSF under 1.0, 18.0 c/d of binoculus and the worse eye (1.0 c/d: r=0.296, 0.292; both at P<0.05; 18.0 c/d: r=0.386, 0.321; both at P<0.05). Conclusions:The qCSF measurements can reflect visual function of patients with early cataract at different spatial frequencies.The qCSF measurements of binoculus, the better eye and the worse eye show significant correlations with the composite scores of QOL.The QOL of early cataract patients shows a strong dependency on the worse eye.