Objective: To study the role of vascular endothelial growth factor (VEGF) during angiogenesis in oral squamous cell carcinoma (SCC).Methods:Surgical specimens from 45 patients with oral SCC were examined for VEGF expression and microvessel density(MVD) by immunocytochemical staining. Anti-VEGF polyclonal antibody was used to determine VEGF expression and anti-CD 34 monoclonal antibody was used to determine MVD. Results:①VEGF protein was mainly found in cytoplasm of cancer cells and in few endothelial cells. Stained microvessels were mainly distributed in the regions of the connective tissue near the cancer nest;②there was a close positive correlation between MVD and VEGF expression;③SCC with high MVD or high VEGF level was mostly found with metastasis in cervical lymph node (P

For optimal adaptive cultivation region selection, we used ecology factors characterized Duolun region as model area to carry out the adaptive habitat division of Paeonia lactiflora. Similar priority comparison of ecology factors.in 91 cities were calculated by Fuzzy methods, then, distance of the ecology factors were transferred to spacial model by geography information system (,GIS) and modified by soil utilization map of China. The results showed that P. lactiflora were mainly distributed in the Daxing'an Mountain, Changbaishan and qinling range which were divided into six grades of suitable regions belonging to three geographical distributed units. The most similar areas to Duolun were Huade, Xilinhaote, Suolun and Zhangbei. P. lactiflora's distribution and quality are relevant with longitude and latitude, and temperature and rainfall.
China ; Environment ; Fuzzy Logic ; Paeonia ; classification ; growth & development ; Temperature